Alignments for a candidate for amaB in Microvirga lotononidis WSM3557

GapMind for catabolism of small carbon sources

Alignments for a candidate for amaB in Microvirga lotononidis WSM3557

Align Putative aldehyde dehydrogenase transmembrane protein; EC 1.2.1.3 (characterized, see rationale)
to candidate WP_009763131.1 MICLODRAFT_RS17515 aldehyde dehydrogenase family protein

Query= uniprot:Q92L07
         (510 letters)



>NCBI__GCF_000262405.1:WP_009763131.1
          Length = 516

 Score =  729 bits (1882), Expect = 0.0
 Identities = 365/508 (71%), Positives = 416/508 (81%)

Query: 3   IAAKKIDVASEAAALLDKMGVAKDLYTGGDMPSFSPVTGEKIASLKTVSAAEAAGKIEKA 62
           +++    VA EA A+L ++GV +  +     P+ SP+TGE IA ++  +  EA   I +A
Sbjct: 8   VSSAPASVADEARAILARLGVPESAFASTGRPALSPITGEVIAHVRETTPDEAKAAIGRA 67

Query: 63  DEAFRAWRLVPAPKRGELVRLLGEELRAFKADLGRLVSIEAGKIPSEGLGEVQEMIDICD 122
           D AF+AWR VPAPKRGE +RLLGEELRA K DLGRLV++EAGKI SEGLGEVQEMIDICD
Sbjct: 68  DAAFKAWRKVPAPKRGEFIRLLGEELRAAKDDLGRLVTLEAGKIVSEGLGEVQEMIDICD 127

Query: 123 FAVGLSRQLYGLTIATERPGHRMMETWHPLGVVGIISAFNFPVAVWSWNAALALVCGDAV 182
           FAVGLSRQLYGLTIATER  HRMMETWHPLGV G+ISAFNFPVAVWSWNAALALVCGD+V
Sbjct: 128 FAVGLSRQLYGLTIATERADHRMMETWHPLGVCGVISAFNFPVAVWSWNAALALVCGDSV 187

Query: 183 VWKPSEKTPLTALACQAILERAIARFGDAPEGLSQVLIGDRAIGEVLVDHPKVPLVSATG 242
           VWKPSEKT LTALA  AI+ERA  RFG  PEGL +VL+G R IGE+LV+  +VP++SATG
Sbjct: 188 VWKPSEKTLLTALATHAIVERAAKRFGGVPEGLCEVLLGGREIGEILVEDHRVPVLSATG 247

Query: 243 STRMGREVGPRLAKRFARAILELGGNNAGIVCPSADLDMALRAIAFGAMGTAGQRCTTLR 302
           ST MGR+VGP+LA+RFARAILELGGNNA IV PSADLD+ALR IAF AMGTAGQRCTTLR
Sbjct: 248 STAMGRQVGPKLAERFARAILELGGNNAAIVAPSADLDLALRGIAFAAMGTAGQRCTTLR 307

Query: 303 RLFVHESVYDQLVPRLKKAYQSVSVGNPLESAALVGPLVDKAAFDGMQKAIAEAKNHGGA 362
           RLFVHESVYDQLVPRL K Y SV +G+P     LVGPL+DKAAFD M++A+ EA+  GG 
Sbjct: 308 RLFVHESVYDQLVPRLVKVYGSVKIGDPRAEGTLVGPLIDKAAFDNMERALDEARAAGGK 367

Query: 363 VTGGERVELGHENGYYVKPALVEMPKQEGPVLEETFAPILYVMKYSDFDAVLAEHNAVAA 422
           V GG R       G Y +PALVEMP Q GPV  ETFAPILYVM+YSDFD V+A HNAV A
Sbjct: 368 VHGGGRYTDVANEGVYARPALVEMPSQTGPVTRETFAPILYVMRYSDFDEVIALHNAVGA 427

Query: 423 GLSSSIFTRDMQESERFLAADGSDCGIANVNIGTSGAEIGGAFGGEKETGGGRESGSDAW 482
           GLSSSIFT +++E+E F++A GSDCGIANVNIG SGAEIGGAFGGEKETGGGRESGSDAW
Sbjct: 428 GLSSSIFTLNLREAEAFVSAAGSDCGIANVNIGPSGAEIGGAFGGEKETGGGRESGSDAW 487

Query: 483 KAYMRRATNTVNYSKALPLAQGVSFDIE 510
           KAYMRRATNT+NY   LPLAQGV FD++
Sbjct: 488 KAYMRRATNTINYGSTLPLAQGVKFDVD 515


Lambda     K      H
   0.317    0.134    0.390 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 828
Number of extensions: 31
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 510
Length of database: 516
Length adjustment: 35
Effective length of query: 475
Effective length of database: 481
Effective search space:   228475
Effective search space used:   228475
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources