GapMind for catabolism of small carbon sources

 

Protein WP_017220929.1 in Moritella dasanensis ArB 0140

Annotation: NCBI__GCF_000276805.1:WP_017220929.1

Length: 394 amino acids

Source: GCF_000276805.1 in NCBI

Candidate for 21 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
D-alanine catabolism Pf6N2E2_5403 hi ABC transporter for D-Alanine, permease component 2 (characterized) 52% 100% 389 ABC transporter for L-Glutamine, L-Histidine, and other L-amino acids, permease component 1 46% 358.6
L-histidine catabolism aapQ med ABC transporter for L-Glutamine, L-Histidine, and other L-amino acids, permease component 1 (characterized) 46% 97% 358.6 ABC transporter for D-Alanine, permease component 2 52% 389.0
L-asparagine catabolism aapQ med AapQ, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) 48% 95% 356.7 ABC transporter for D-Alanine, permease component 2 52% 389.0
L-aspartate catabolism aapQ med AapQ, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) 48% 95% 356.7 ABC transporter for D-Alanine, permease component 2 52% 389.0
L-glutamate catabolism aapQ med AapQ, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) 48% 95% 356.7 ABC transporter for D-Alanine, permease component 2 52% 389.0
L-leucine catabolism aapQ med AapQ, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) 48% 95% 356.7 ABC transporter for D-Alanine, permease component 2 52% 389.0
L-proline catabolism aapQ med AapQ, component of General L-amino acid porter; transports basic and acidic amino acids preferentially, but also transports aliphatic amino acids (catalyzes both uptake and efflux) (characterized) 48% 95% 356.7 ABC transporter for D-Alanine, permease component 2 52% 389.0
L-asparagine catabolism bztB lo glutamate/glutamine/aspartate/asparagine transport system permease protein BztB (characterized) 37% 99% 268.9 ABC transporter for D-Alanine, permease component 2 52% 389.0
L-aspartate catabolism bztB lo glutamate/glutamine/aspartate/asparagine transport system permease protein BztB (characterized) 37% 99% 268.9 ABC transporter for D-Alanine, permease component 2 52% 389.0
L-glutamate catabolism bztB lo glutamate/glutamine/aspartate/asparagine transport system permease protein BztB (characterized) 37% 99% 268.9 ABC transporter for D-Alanine, permease component 2 52% 389.0
L-asparagine catabolism bgtB' lo ABC-type permease for basic amino acids and glutamine (characterized, see rationale) 35% 94% 230.3 ABC transporter for D-Alanine, permease component 2 52% 389.0
L-aspartate catabolism bgtB' lo ABC-type permease for basic amino acids and glutamine (characterized, see rationale) 35% 94% 230.3 ABC transporter for D-Alanine, permease component 2 52% 389.0
L-asparagine catabolism natG lo NatG, component of Acidic and neutral amino acid uptake transporter NatFGH/BgtA. BgtA is shared with BgtAB (characterized) 46% 63% 144.1 ABC transporter for D-Alanine, permease component 2 52% 389.0
L-aspartate catabolism natG lo NatG, component of Acidic and neutral amino acid uptake transporter NatFGH/BgtA. BgtA is shared with BgtAB (characterized) 46% 63% 144.1 ABC transporter for D-Alanine, permease component 2 52% 389.0
L-asparagine catabolism aatQ lo Glutamate/aspartate import permease protein GltJ (characterized) 34% 68% 89 ABC transporter for D-Alanine, permease component 2 52% 389.0
L-aspartate catabolism aatQ lo Glutamate/aspartate import permease protein GltJ (characterized) 34% 68% 89 ABC transporter for D-Alanine, permease component 2 52% 389.0
L-glutamate catabolism gltJ lo Glutamate/aspartate import permease protein GltJ (characterized) 34% 68% 89 ABC transporter for D-Alanine, permease component 2 52% 389.0
L-glutamate catabolism gluC lo GluC aka CGL1952, component of Glutamate porter (characterized) 31% 56% 77.4 ABC transporter for D-Alanine, permease component 2 52% 389.0
D-glucosamine (chitosamine) catabolism AO353_21720 lo ABC transporter for D-Glucosamine, permease component 1 (characterized) 34% 54% 70.9 ABC transporter for D-Alanine, permease component 2 52% 389.0
L-asparagine catabolism peb1D lo Amino acid ABC transporter, permease protein PEB1 (characterized, see rationale) 30% 60% 69.3 ABC transporter for D-Alanine, permease component 2 52% 389.0
L-aspartate catabolism peb1D lo Amino acid ABC transporter, permease protein PEB1 (characterized, see rationale) 30% 60% 69.3 ABC transporter for D-Alanine, permease component 2 52% 389.0

Sequence Analysis Tools

View WP_017220929.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MNNSKLPPTSSGLFNSPQNRAIIFQVLSLVVVVLCIFYFVNNMFDNVAKRGITTGFSFLG
ETAGFGVSQALIPYDDTSSTFLDVFIVGLLNTMLVGVIGIVLASIIGLMVGIGRLSSNYL
IAKLSLVYIETFRNIPILLQILFWYNVVLAALPSPRQSISYFDSVFINNRGLIMPDPIFE
SGSGFILVAFILACISVVFLAKWASKRHDLTGEEFPLIRVSLAIVVVAPLLVFFVTGQPI
SAEYPALKGFNFKGGITIIPELLALIFALSIYTATYIAEAVRAGIEAVPPGQKEAAKSLG
LKEHVILRKVVLPQALRVIIPPVINQYLNLVKNSSLATAIGYPEIVTLFSGTTLNQVGQA
IEIILMTMAVYLVFSIVISLLLNWVNAKMEIKGR

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory