Alignments for a candidate for gguA in Moritella dasanensis ArB 0140

GapMind for catabolism of small carbon sources

Alignments for a candidate for gguA in Moritella dasanensis ArB 0140

Align GguA aka ATU2347 aka AGR_C_4264, component of Multiple sugar (arabinose, xylose, galactose, glucose, fucose) putative porter (characterized)
to candidate WP_017221119.1 A923_RS0107850 ribose ABC transporter ATP-binding protein RbsA

Query= TCDB::O05176
         (512 letters)



>NCBI__GCF_000276805.1:WP_017221119.1
          Length = 501

 Score =  409 bits (1050), Expect = e-118
 Identities = 226/502 (45%), Positives = 324/502 (64%), Gaps = 15/502 (2%)

Query: 1   MANTILEMRNITKTFPGVKALENVNLKVKEGEIHALVGENGAGKSTLMKVLSGVYPAGTY 60
           M   IL++  I K+FPGVKAL+N  L V  G++ AL+GENGAGKSTLMKVL+G+Y     
Sbjct: 1   MTQAILKLSGIEKSFPGVKALDNACLNVYPGKVMALLGENGAGKSTLMKVLTGIYHMD-- 58

Query: 61  EGEIHYEGAVRNFRAINDSEDIGIIIIHQELALVPLLSIAENIFLGNEVASN-GVISWQQ 119
           +G I+Y+G+   F     S+++GI IIHQEL L+P L+IAENI+LG E  +  G I W Q
Sbjct: 59  KGSINYQGSDVTFDGPRHSQEVGISIIHQELNLIPELTIAENIYLGRETTNAFGGIKWTQ 118

Query: 120 TFNRTRELLKKVGLKESPETLITDIGVGKQQLVEIAKALSKSVKLLILDEPTASLNESDS 179
            F     LL+++ +K S   L+ ++ +G+QQ+VEIAKALS   +++++DEPT +L ES++
Sbjct: 119 MFADADALLQRLNVKHSSRQLLGELSLGEQQMVEIAKALSFKSQVIVMDEPTDALTESET 178

Query: 180 EALLNLLMEFRNQGMTSIIITHKLNEVRKVADQITVLRDGMTVKTLDCHQEEISEDVIIR 239
           ++L  ++ E RN+G   + I+H+L E+ ++ D ITVLRDG  +  +     +I ED +I 
Sbjct: 179 KSLFKVINELRNEGCGIVYISHRLKEIFEICDDITVLRDGKFIAEIAV--TDIDEDGLIE 236

Query: 240 NMVGRDLEDRYPPRDVPIGETILEVKNWNAYHQQHRDRQVLHDINVTVRKGEVVGIAGLM 299
            MVGR L++ YP  D   G   LEV+N  A          + D++ T+  GE++GI+GLM
Sbjct: 237 KMVGRRLDEIYPRIDAKHGTLCLEVENIVA--------PGVRDVSFTLDHGEILGISGLM 288

Query: 300 GAGRTEFAMSVFGKSYGHRITGDVLIDGKPVDVSTVRKAIDAGLAYVTEDRKHLGLVLND 359
           GAGRTE   +++G     R +GDV++D K V   T R  +  G+AY++EDRK  GL+L  
Sbjct: 289 GAGRTELMKAIYGAL--PRQSGDVILDDKVVSPITPRDGLANGIAYISEDRKGDGLILGL 346

Query: 360 NILHNTTLANLAGVSKASIIDDIKEMKVASDFRTRLRIRSSGIFQETVNLSGGNQQKVVL 419
           ++  N +L  L  +SK   +D  KE     DF  +  +++    Q   NLSGGNQQKV +
Sbjct: 347 SVKENMSLCALDALSKGLQLDHAKEATAVEDFMRQFNVKTPSRDQIIGNLSGGNQQKVAI 406

Query: 420 SKWLFSNPDVLILDEPTRGIDVGAKYEIYTIINQLAADGKGVLMISSEMPELLGNCDRIY 479
           +K L + P VLILDEPTRG+DVGAK EIY +INQ  A+G  ++++SSEMPE+LG  DRI 
Sbjct: 407 AKGLMTRPKVLILDEPTRGVDVGAKKEIYQLINQFKAEGMSIILVSSEMPEVLGMSDRIL 466

Query: 480 VMNEGRIVAELPKGEASQESIM 501
           VM+EGRI  E    +A+QE +M
Sbjct: 467 VMHEGRISGEFMAADANQEKLM 488



 Score = 72.0 bits (175), Expect = 5e-17
 Identities = 57/230 (24%), Positives = 107/230 (46%), Gaps = 11/230 (4%)

Query: 278 QVLHDINVTVRKGEVVGIAGLMGAGRTEFAMSVFGKSYGHRITGDVLIDGKPVDVSTVRK 337
           + L +  + V  G+V+ + G  GAG++   M V    Y H   G +   G  V     R 
Sbjct: 19  KALDNACLNVYPGKVMALLGENGAGKSTL-MKVLTGIY-HMDKGSINYQGSDVTFDGPRH 76

Query: 338 AIDAGLAYVTEDRKHLG-LVLNDNI-LHNTTLANLAGVSKASIIDDIKEMKVASDFRTRL 395
           + + G++ + ++   +  L + +NI L   T     G+    +  D      A     RL
Sbjct: 77  SQEVGISIIHQELNLIPELTIAENIYLGRETTNAFGGIKWTQMFAD------ADALLQRL 130

Query: 396 RIRSSGIFQETVNLSGGNQQKVVLSKWLFSNPDVLILDEPTRGIDVGAKYEIYTIINQLA 455
            ++ S   Q    LS G QQ V ++K L     V+++DEPT  +       ++ +IN+L 
Sbjct: 131 NVKHSSR-QLLGELSLGEQQMVEIAKALSFKSQVIVMDEPTDALTESETKSLFKVINELR 189

Query: 456 ADGKGVLMISSEMPELLGNCDRIYVMNEGRIVAELPKGEASQESIMRAIM 505
            +G G++ IS  + E+   CD I V+ +G+ +AE+   +  ++ ++  ++
Sbjct: 190 NEGCGIVYISHRLKEIFEICDDITVLRDGKFIAEIAVTDIDEDGLIEKMV 239


Lambda     K      H
   0.316    0.135    0.374 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 679
Number of extensions: 35
Number of successful extensions: 9
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 512
Length of database: 501
Length adjustment: 34
Effective length of query: 478
Effective length of database: 467
Effective search space:   223226
Effective search space used:   223226
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources