Alignments for a candidate for tctA in Moritella dasanensis ArB 0140

GapMind for catabolism of small carbon sources

Alignments for a candidate for tctA in Moritella dasanensis ArB 0140

Align Tricarboxylate transport membrane protein TctA (characterized)
to candidate WP_017220569.1 A923_RS0105045 tripartite tricarboxylate transporter permease

Query= reanno::Dino:3609738
         (505 letters)



>NCBI__GCF_000276805.1:WP_017220569.1
          Length = 511

 Score =  589 bits (1519), Expect = e-173
 Identities = 294/503 (58%), Positives = 385/503 (76%), Gaps = 9/503 (1%)

Query: 1   MLEGLLIGLQTAFSIQNLAMVIGGCLIGTFIGMLPGLGPMSIIAIMIPVAISLGDPSAAL 60
           ML+G+L GL TA    NL MV+ GC +GTFIGMLPGLGP+S IA+MIP+   L DPS+ +
Sbjct: 1   MLDGILAGLSTAIMPTNLMMVMVGCFVGTFIGMLPGLGPISAIALMIPITYGL-DPSSGM 59

Query: 61  ILLAGVYYGAIFGGSTSSILLNAPGVAGTVATSFDGYPMAQQGKAGKALTIAAIASFAGG 120
           IL+AGVYYGAIFGGSTSSIL+NAPG + TV T+FDGYP+A++G+AGKAL +AA ASF GG
Sbjct: 60  ILMAGVYYGAIFGGSTSSILINAPGCSSTVVTAFDGYPLAKKGQAGKALALAAYASFTGG 119

Query: 121 TIGAILLMVFAPALSSVALLFHSAEYFALMVVGLSAIAAFAGTGQVAKALLMTILGLIMA 180
           T+ AI+L++ APAL+ V+L F S++YFALM+VGLSA+AAFAG GQV KA +MTI GL+++
Sbjct: 120 TLSAIMLLIAAPALARVSLSFQSSDYFALMLVGLSAVAAFAGKGQVLKAWMMTIFGLMLS 179

Query: 181 TVGEGALFASPRFTMGLMDLQSGFGFITLAMAMFALPEALFLVMNPLRAASGQGGG---E 237
           TVG        RFT GL DL  GF F+ LAMA FAL E LF ++ P    S +      E
Sbjct: 180 TVGIDKGIGVERFTFGLTDLMDGFSFLLLAMATFALGEILFSILKPEPDTSAEENSALSE 239

Query: 238 IKDLRITRAEARSIAPVIGRQSVQGFFIGVLPGAGATIASFLGYAVERNIASKDEQAEFG 297
           I  +++T+ E + +APV  R S+ GFF+GVLPGAGATIA+FL Y +ERN+A KD++ EFG
Sbjct: 240 IGSMKVTKEEFKEVAPVAIRSSILGFFVGVLPGAGATIAAFLSYGLERNLAPKDKRDEFG 299

Query: 298 KGSVKGLAAPETANNAACTGSFVPLLTLGIPGSGTTAILLGALLALNVSPGPRLMIDAPE 357
           KGS++GL APE ANNAA +GSFVPLLTLGIPGSGTTAI+LGA+++  + PGPRL +D PE
Sbjct: 300 KGSIRGLVAPEAANNAASSGSFVPLLTLGIPGSGTTAIMLGAMISYGIQPGPRLFVDNPE 359

Query: 358 IFWAVIMSMFIGNLVLLILNLPLIPYIAKILSVPRNYLIPFILFFTLMGAYIGQNNATEL 417
           IFW+VI+SM+ GNLVL++LNLPLIPYIAK+L+VPR  L+P I+FF++ G Y+   N  ++
Sbjct: 360 IFWSVIISMYFGNLVLMVLNLPLIPYIAKLLAVPRTVLLPMIIFFSITGVYLVSFNTVDV 419

Query: 418 LLLVGFGICATILKFADYPLAPLLIGFILGGLLENNFSRAMQLYDG-ISFIWERPMTLGL 476
            +++   + A  L+ A +PLAPLL+GFILGGL+E N  R++ + DG +SF+WERP+TL  
Sbjct: 420 FIMILVAVIAIFLRLATFPLAPLLLGFILGGLMEENLRRSLMISDGELSFLWERPITLTF 479

Query: 477 LVIAALLIILP----SYRNRRAK 495
            VI+AL++I P    ++  RR K
Sbjct: 480 TVISALVLITPLLLTAFNRRRVK 502


Lambda     K      H
   0.326    0.143    0.411 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 809
Number of extensions: 44
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 505
Length of database: 511
Length adjustment: 34
Effective length of query: 471
Effective length of database: 477
Effective search space:   224667
Effective search space used:   224667
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.6 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources