Alignments for a candidate for mglA in Moritella dasanensis ArB 0140

GapMind for catabolism of small carbon sources

Alignments for a candidate for mglA in Moritella dasanensis ArB 0140

Align Galactose/methyl galactoside import ATP-binding protein MglA; EC 7.5.2.11 (characterized)
to candidate WP_017221119.1 A923_RS0107850 ribose ABC transporter ATP-binding protein RbsA

Query= SwissProt::P23924
         (506 letters)



>NCBI__GCF_000276805.1:WP_017221119.1
          Length = 501

 Score =  422 bits (1086), Expect = e-122
 Identities = 218/491 (44%), Positives = 328/491 (66%), Gaps = 5/491 (1%)

Query: 13  LLEMRGINKSFPGVKALDNVNLNVRPHSIHALMGENGAGKSTLLKCLFGIYQKDSGSIVF 72
           +L++ GI KSFPGVKALDN  LNV P  + AL+GENGAGKSTL+K L GIY  D GSI +
Sbjct: 5   ILKLSGIEKSFPGVKALDNACLNVYPGKVMALLGENGAGKSTLMKVLTGIYHMDKGSINY 64

Query: 73  QGKEVDFHSAKEALENGISMVHQELNLVLQRSVMDNMWLGRYPTKGMF-VDQDKMYQDTK 131
           QG +V F   + + E GIS++HQELNL+ + ++ +N++LGR  T     +   +M+ D  
Sbjct: 65  QGSDVTFDGPRHSQEVGISIIHQELNLIPELTIAENIYLGRETTNAFGGIKWTQMFADAD 124

Query: 132 AIFDELDIDIDPRARVGTLSVSQMQMIEIAKAFSYNAKIVIMDEPTSSLTEKEVNHLFTI 191
           A+   L++    R  +G LS+ + QM+EIAKA S+ +++++MDEPT +LTE E   LF +
Sbjct: 125 ALLQRLNVKHSSRQLLGELSLGEQQMVEIAKALSFKSQVIVMDEPTDALTESETKSLFKV 184

Query: 192 IRKLKERGCGIVYISHKMEEIFQLCDEITILRDGQWIATQPLEGLDMDKIIAMMVGRSLN 251
           I +L+  GCGIVYISH+++EIF++CD+IT+LRDG++IA   +  +D D +I  MVGR L+
Sbjct: 185 INELRNEGCGIVYISHRLKEIFEICDDITVLRDGKFIAEIAVTDIDEDGLIEKMVGRRLD 244

Query: 252 QRFPDKENKPGDVILEVRHLTSLRQPSIRDVSFDLHKGEILGIAGLVGAKRTDIVETLFG 311
           + +P  + K G + LEV ++ +   P +RDVSF L  GEILGI+GL+GA RT++++ ++G
Sbjct: 245 EIYPRIDAKHGTLCLEVENIVA---PGVRDVSFTLDHGEILGISGLMGAGRTELMKAIYG 301

Query: 312 IREKSSGTITLHGKKINNHTANEAINHGFALVTEERRSTGIYAYLDIGFNSLISNIRNYK 371
              + SG + L  K ++  T  + + +G A ++E+R+  G+   L +  N  +  + +  
Sbjct: 302 ALPRQSGDVILDDKVVSPITPRDGLANGIAYISEDRKGDGLILGLSVKENMSLCAL-DAL 360

Query: 372 NKVGLLDNSRMKSDTQWVIDSMRVKTPGHRTQIGSLSGGNQQKVIIGRWLLTQPEILMLD 431
           +K   LD+++  +  +  +    VKTP     IG+LSGGNQQKV I + L+T+P++L+LD
Sbjct: 361 SKGLQLDHAKEATAVEDFMRQFNVKTPSRDQIIGNLSGGNQQKVAIAKGLMTRPKVLILD 420

Query: 432 EPTRGIDVGAKFEIYQLIAELAKKGKGIIIISSEMPELLGITDRILVMSNGLVSGIVDTK 491
           EPTRG+DVGAK EIYQLI +   +G  II++SSEMPE+LG++DRILVM  G +SG     
Sbjct: 421 EPTRGVDVGAKKEIYQLINQFKAEGMSIILVSSEMPEVLGMSDRILVMHEGRISGEFMAA 480

Query: 492 TTTQNEILRLA 502
              Q +++  A
Sbjct: 481 DANQEKLMACA 491



 Score = 95.9 bits (237), Expect = 3e-24
 Identities = 65/254 (25%), Positives = 122/254 (48%), Gaps = 11/254 (4%)

Query: 5   ISPPSGEYLLEMRGINKSFPGVKALDNVNLNVRPHSIHALMGENGAGKSTLLKCLFGIYQ 64
           I    G   LE+  I    PGV+   +V+  +    I  + G  GAG++ L+K ++G   
Sbjct: 250 IDAKHGTLCLEVENIVA--PGVR---DVSFTLDHGEILGISGLMGAGRTELMKAIYGALP 304

Query: 65  KDSGSIVFQGKEVDFHSAKEALENGISMVHQELN---LVLQRSVMDNMWLGRYP--TKGM 119
           + SG ++   K V   + ++ L NGI+ + ++     L+L  SV +NM L      +KG+
Sbjct: 305 RQSGDVILDDKVVSPITPRDGLANGIAYISEDRKGDGLILGLSVKENMSLCALDALSKGL 364

Query: 120 FVDQDKMYQDTKAIFDELDIDIDPRARV-GTLSVSQMQMIEIAKAFSYNAKIVIMDEPTS 178
            +D  K     +    + ++    R ++ G LS    Q + IAK      K++I+DEPT 
Sbjct: 365 QLDHAKEATAVEDFMRQFNVKTPSRDQIIGNLSGGNQQKVAIAKGLMTRPKVLILDEPTR 424

Query: 179 SLTEKEVNHLFTIIRKLKERGCGIVYISHKMEEIFQLCDEITILRDGQWIATQPLEGLDM 238
            +       ++ +I + K  G  I+ +S +M E+  + D I ++ +G+          + 
Sbjct: 425 GVDVGAKKEIYQLINQFKAEGMSIILVSSEMPEVLGMSDRILVMHEGRISGEFMAADANQ 484

Query: 239 DKIIAMMVGRSLNQ 252
           +K++A  VG+ +N+
Sbjct: 485 EKLMACAVGKKINE 498


Lambda     K      H
   0.319    0.137    0.388 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 670
Number of extensions: 33
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 506
Length of database: 501
Length adjustment: 34
Effective length of query: 472
Effective length of database: 467
Effective search space:   220424
Effective search space used:   220424
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources