Alignments for a candidate for mglA in Moritella dasanensis ArB 0140

GapMind for catabolism of small carbon sources

Alignments for a candidate for mglA in Moritella dasanensis ArB 0140

Align Monosaccharide-transporting ATPase, component of Glucose porter. Also bind xylose (Boucher and Noll 2011). Induced by glucose (Frock et al. 2012). Directly regulated by glucose-responsive regulator GluR (characterized)
to candidate WP_017221119.1 A923_RS0107850 ribose ABC transporter ATP-binding protein RbsA

Query= TCDB::G4FGN3
         (494 letters)



>NCBI__GCF_000276805.1:WP_017221119.1
          Length = 501

 Score =  424 bits (1091), Expect = e-123
 Identities = 215/490 (43%), Positives = 334/490 (68%), Gaps = 2/490 (0%)

Query: 4   ILEVKSIHKRFPGVHALKGVSMEFYPGEVHAIVGENGAGKSTLMKIIAGVYQPDEGEIIY 63
           IL++  I K FPGV AL    +  YPG+V A++GENGAGKSTLMK++ G+Y  D+G I Y
Sbjct: 5   ILKLSGIEKSFPGVKALDNACLNVYPGKVMALLGENGAGKSTLMKVLTGIYHMDKGSINY 64

Query: 64  EGRGVRWNHPSEAINAGIVTVFQELSVMDNLSVAENIFMGDEEKRGIF-IDYKKMYREAE 122
           +G  V ++ P  +   GI  + QEL+++  L++AENI++G E       I + +M+ +A+
Sbjct: 65  QGSDVTFDGPRHSQEVGISIIHQELNLIPELTIAENIYLGRETTNAFGGIKWTQMFADAD 124

Query: 123 KFMKEEFGIEIDPEEKLGKYSIAIQQMVEIARAVYKKAKVLILDEPTSSLTQKETEKLFE 182
             + +   ++    + LG+ S+  QQMVEIA+A+  K++V+++DEPT +LT+ ET+ LF+
Sbjct: 125 ALL-QRLNVKHSSRQLLGELSLGEQQMVEIAKALSFKSQVIVMDEPTDALTESETKSLFK 183

Query: 183 VVKSLKEKGVAIIFISHRLEEIFEICDKVSVLRDGEYIGTDSIENLTKEKIVEMMVGRKL 242
           V+  L+ +G  I++ISHRL+EIFEICD ++VLRDG++I   ++ ++ ++ ++E MVGR+L
Sbjct: 184 VINELRNEGCGIVYISHRLKEIFEICDDITVLRDGKFIAEIAVTDIDEDGLIEKMVGRRL 243

Query: 243 EKFYIKEAHEPGEVVLEVKNLSGERFENVSFSLRRGEILGFAGLVGAGRTELMETIFGFR 302
           ++ Y +   + G + LEV+N+      +VSF+L  GEILG +GL+GAGRTELM+ I+G  
Sbjct: 244 DEIYPRIDAKHGTLCLEVENIVAPGVRDVSFTLDHGEILGISGLMGAGRTELMKAIYGAL 303

Query: 303 PKRGGEIYIEGKRVEINHPLDAIEQGIGLVPEDRKKLGLILIMSIMHNVSLPSLDRIKKG 362
           P++ G++ ++ K V    P D +  GI  + EDRK  GLIL +S+  N+SL +LD + KG
Sbjct: 304 PRQSGDVILDDKVVSPITPRDGLANGIAYISEDRKGDGLILGLSVKENMSLCALDALSKG 363

Query: 363 PFISFKREKELADWAIKTFDIRPAYPDRKVLYLSGGNQQKVVLAKWLALKPKILILDEPT 422
             +   +E    +  ++ F+++    D+ +  LSGGNQQKV +AK L  +PK+LILDEPT
Sbjct: 364 LQLDHAKEATAVEDFMRQFNVKTPSRDQIIGNLSGGNQQKVAIAKGLMTRPKVLILDEPT 423

Query: 423 RGIDVGAKAEIYRIMSQLAKEGVGVIMISSELPEVLQMSDRIAVMSFGKLAGIIDAKEAS 482
           RG+DVGAK EIY++++Q   EG+ +I++SSE+PEVL MSDRI VM  G+++G   A +A+
Sbjct: 424 RGVDVGAKKEIYQLINQFKAEGMSIILVSSEMPEVLGMSDRILVMHEGRISGEFMAADAN 483

Query: 483 QEKVMKLAAG 492
           QEK+M  A G
Sbjct: 484 QEKLMACAVG 493



 Score =  102 bits (255), Expect = 2e-26
 Identities = 71/246 (28%), Positives = 130/246 (52%), Gaps = 12/246 (4%)

Query: 5   LEVKSIHKRFPGVHALKGVSMEFYPGEVHAIVGENGAGKSTLMKIIAGVYQPDEGEIIYE 64
           LEV++I    PGV   + VS     GE+  I G  GAG++ LMK I G      G++I +
Sbjct: 259 LEVENIVA--PGV---RDVSFTLDHGEILGISGLMGAGRTELMKAIYGALPRQSGDVILD 313

Query: 65  GRGVRWNHPSEAINAGIVTVFQELS---VMDNLSVAENIFMG--DEEKRGIFIDYKKMYR 119
            + V    P + +  GI  + ++     ++  LSV EN+ +   D   +G+ +D+ K   
Sbjct: 314 DKVVSPITPRDGLANGIAYISEDRKGDGLILGLSVKENMSLCALDALSKGLQLDHAKEAT 373

Query: 120 EAEKFMKEEFGIEIDPEEKL-GKYSIAIQQMVEIARAVYKKAKVLILDEPTSSLTQKETE 178
             E FM++ F ++    +++ G  S   QQ V IA+ +  + KVLILDEPT  +     +
Sbjct: 374 AVEDFMRQ-FNVKTPSRDQIIGNLSGGNQQKVAIAKGLMTRPKVLILDEPTRGVDVGAKK 432

Query: 179 KLFEVVKSLKEKGVAIIFISHRLEEIFEICDKVSVLRDGEYIGTDSIENLTKEKIVEMMV 238
           ++++++   K +G++II +S  + E+  + D++ V+ +G   G     +  +EK++   V
Sbjct: 433 EIYQLINQFKAEGMSIILVSSEMPEVLGMSDRILVMHEGRISGEFMAADANQEKLMACAV 492

Query: 239 GRKLEK 244
           G+K+ +
Sbjct: 493 GKKINE 498


Lambda     K      H
   0.318    0.138    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 664
Number of extensions: 43
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 494
Length of database: 501
Length adjustment: 34
Effective length of query: 460
Effective length of database: 467
Effective search space:   214820
Effective search space used:   214820
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources