Alignments for a candidate for PS417_12065 in Moritella dasanensis ArB 0140

GapMind for catabolism of small carbon sources

Alignments for a candidate for PS417_12065 in Moritella dasanensis ArB 0140

Align D-ribose transporter ATP-binding protein; SubName: Full=Putative xylitol transport system ATP-binding protein; SubName: Full=Sugar ABC transporter ATP-binding protein (characterized, see rationale)
to candidate WP_017221119.1 A923_RS0107850 ribose ABC transporter ATP-binding protein RbsA

Query= uniprot:A0A1N7TX47
         (495 letters)



>NCBI__GCF_000276805.1:WP_017221119.1
          Length = 501

 Score =  352 bits (903), Expect = e-101
 Identities = 207/493 (41%), Positives = 289/493 (58%), Gaps = 10/493 (2%)

Query: 6   LLQAEHVAKAYAGVPALRDGRLSLRAGSVHALCGGNGAGKSTFLSILMGITQRDAGSILL 65
           +L+   + K++ GV AL +  L++  G V AL G NGAGKST + +L GI   D GSI  
Sbjct: 5   ILKLSGIEKSFPGVKALDNACLNVYPGKVMALLGENGAGKSTLMKVLTGIYHMDKGSINY 64

Query: 66  NGAPVQFNRPSEALAAGIAMITQELEPIPYMTVAENIWLGREPRRAGCIVDNKALNRRTR 125
            G+ V F+ P  +   GI++I QEL  IP +T+AENI+LGRE   A   +    +     
Sbjct: 65  QGSDVTFDGPRHSQEVGISIIHQELNLIPELTIAENIYLGRETTNAFGGIKWTQMFADAD 124

Query: 126 ELLDSLEFDVDATSPMHRLSVAQIQLVEIAKAFSHDCQVMIMDEPTSAIGEHEAQTLFKA 185
            LL  L     +   +  LS+ + Q+VEIAKA S   QV++MDEPT A+ E E ++LFK 
Sbjct: 125 ALLQRLNVKHSSRQLLGELSLGEQQMVEIAKALSFKSQVIVMDEPTDALTESETKSLFKV 184

Query: 186 IRRLTAQGAGIVYVSHRLSELAQIADDYSIFRDGAFVESGRMADIDRDHLVRGIVGQELT 245
           I  L  +G GIVY+SHRL E+ +I DD ++ RDG F+    + DID D L+  +VG+ L 
Sbjct: 185 INELRNEGCGIVYISHRLKEIFEICDDITVLRDGKFIAEIAVTDIDEDGLIEKMVGRRLD 244

Query: 246 ----RIDHKVGRECAANTCLQVDNLSRAGEFHDISLQLRQGEILGIYGLMGSGRSEFLNC 301
               RID K G       CL+V+N+   G   D+S  L  GEILGI GLMG+GR+E +  
Sbjct: 245 EIYPRIDAKHG-----TLCLEVENIVAPG-VRDVSFTLDHGEILGISGLMGAGRTELMKA 298

Query: 302 IYGLTVADSGSVTLQGKPMPIGLPKATINAGMSLVTEDRKDSGLVLTGSILSNIALSAYK 361
           IYG     SG V L  K +    P+  +  G++ ++EDRK  GL+L  S+  N++L A  
Sbjct: 299 IYGALPRQSGDVILDDKVVSPITPRDGLANGIAYISEDRKGDGLILGLSVKENMSLCALD 358

Query: 362 RLSSWSLINARKETQLAEDMVKRLQIKTTSLELPVASMSGGNQQKVVLAKCLSTEPVCLL 421
            LS    ++  KE    ED +++  +KT S +  + ++SGGNQQKV +AK L T P  L+
Sbjct: 359 ALSKGLQLDHAKEATAVEDFMRQFNVKTPSRDQIIGNLSGGNQQKVAIAKGLMTRPKVLI 418

Query: 422 CDEPTRGIDEGAKQEIYHLLDQFVRGGGAAIVVSSEAPELLHLSDRIAVFKGGRLVTIST 481
            DEPTRG+D GAK+EIY L++QF   G + I+VSSE PE+L +SDRI V   GR+     
Sbjct: 419 LDEPTRGVDVGAKKEIYQLINQFKAEGMSIILVSSEMPEVLGMSDRILVMHEGRISGEFM 478

Query: 482 DTALSQEALLRLA 494
               +QE L+  A
Sbjct: 479 AADANQEKLMACA 491



 Score = 73.9 bits (180), Expect = 1e-17
 Identities = 61/247 (24%), Positives = 116/247 (46%), Gaps = 14/247 (5%)

Query: 5   LLLQAEHVAKAYAGVPALRDGRLSLRAGSVHALCGGNGAGKSTFLSILMGITQRDAGSIL 64
           L L+ E++       P +RD   +L  G +  + G  GAG++  +  + G   R +G ++
Sbjct: 257 LCLEVENIV-----APGVRDVSFTLDHGEILGISGLMGAGRTELMKAIYGALPRQSGDVI 311

Query: 65  LNGAPVQFNRPSEALAAGIAMITQELEP---IPYMTVAENIWL-GREPRRAGCIVDNKAL 120
           L+   V    P + LA GIA I+++ +    I  ++V EN+ L   +    G  +D+   
Sbjct: 312 LDDKVVSPITPRDGLANGIAYISEDRKGDGLILGLSVKENMSLCALDALSKGLQLDHAKE 371

Query: 121 NRRTRELLDSLEFDVDATSP---MHRLSVAQIQLVEIAKAFSHDCQVMIMDEPTSAIGEH 177
                + +   +F+V   S    +  LS    Q V IAK      +V+I+DEPT  +   
Sbjct: 372 ATAVEDFMR--QFNVKTPSRDQIIGNLSGGNQQKVAIAKGLMTRPKVLILDEPTRGVDVG 429

Query: 178 EAQTLFKAIRRLTAQGAGIVYVSHRLSELAQIADDYSIFRDGAFVESGRMADIDRDHLVR 237
             + +++ I +  A+G  I+ VS  + E+  ++D   +  +G        AD +++ L+ 
Sbjct: 430 AKKEIYQLINQFKAEGMSIILVSSEMPEVLGMSDRILVMHEGRISGEFMAADANQEKLMA 489

Query: 238 GIVGQEL 244
             VG+++
Sbjct: 490 CAVGKKI 496


Lambda     K      H
   0.319    0.135    0.381 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 566
Number of extensions: 20
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 495
Length of database: 501
Length adjustment: 34
Effective length of query: 461
Effective length of database: 467
Effective search space:   215287
Effective search space used:   215287
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources