Alignments for a candidate for prpE in Rhizobium grahamii CCGE 502

GapMind for catabolism of small carbon sources

Alignments for a candidate for prpE in Rhizobium grahamii CCGE 502

Align Propionate--CoA ligase (EC 6.2.1.17) (characterized)
to candidate WP_016556838.1 RGCCGE502_RS24215 propionyl-CoA synthetase

Query= reanno::Phaeo:GFF1175
         (629 letters)



>NCBI__GCF_000298315.2:WP_016556838.1
          Length = 636

 Score =  803 bits (2074), Expect = 0.0
 Identities = 382/628 (60%), Positives = 461/628 (73%), Gaps = 4/628 (0%)

Query: 3   YSEVYEGWKANPEQFWMEAAEAISWDSAPTKALTDKGDGLYEWFADARVNTCYNAVDRHV 62
           Y   Y  WK +PE FW EAA  I W   P    + +G     WF     NTCYN +DRHV
Sbjct: 5   YQRTYARWKDDPEAFWQEAAAEIDWFKEPELTFSREGGPYGRWFPGGETNTCYNCLDRHV 64

Query: 63  EQGRGEQTAIIYDSPITHTKREISYVELRNRVATLAGALRAKGVEKGDRVIIYMPMIPEA 122
             GRG  TA IYDSPI   K   SY E+   V+ +A  LR +G+ KGDRVIIYMPM+P+A
Sbjct: 65  RAGRGGDTAFIYDSPIGGAKTIFSYGEVLAEVSAIAATLRERGIGKGDRVIIYMPMVPQA 124

Query: 123 LEAMLACARLGAVHSVVFGGFAANELAVRIDDATPKAIIAASCGLEPGRTVHYKPLLDGA 182
           + +MLACAR+GAVHSVVFGGFAANELA RIDD+  K I+ ASCGLEPGR V YKPL+D A
Sbjct: 125 VFSMLACARIGAVHSVVFGGFAANELAARIDDSGAKLIVTASCGLEPGRVVPYKPLVDQA 184

Query: 183 IDLATHKPDFCVIFQREQEVAELIEGRDVNWHGF---QYGVEPAECVPVEGNHPAYILYT 239
           I +A  KP  C++ QR Q  A+L++GRD ++        GV+  ECV +      YILYT
Sbjct: 185 IAIAKSKPAHCLVLQRPQLAADLVDGRDEDFEAAVARNRGVK-VECVSLGATDELYILYT 243

Query: 240 SGTTGQPKGVIRHTAGQLVALNWTMKNIYNVDPGDVFWAASDVGWVVGHSYICYGPLIHG 299
           SGTTGQPKGV+R   G +VALNW+M NI+ +  G+VFWAASD+GWVVGHSYI Y PL+ G
Sbjct: 244 SGTTGQPKGVVRDNGGHMVALNWSMANIFGIRQGEVFWAASDIGWVVGHSYIVYAPLLAG 303

Query: 300 NTTIVFEGKPIGTPDAGTFWRVISEHKVKSFFTAPTAFRAVKREDPKGEFVKKYDLSCLK 359
            T+++FEGKP+GTPDAG FWR+ SEH V+  FTAPTAFRA++RED +G  +K Y +   +
Sbjct: 304 ATSVIFEGKPVGTPDAGVFWRIASEHDVRVLFTAPTAFRAIRREDSEGVHLKTYSMPNFR 363

Query: 360 QVYLAGERADPDTITWAQEQLKVPVIDHWWQTETGWSIAANPLGIEELPTKLGSPAVPMP 419
            ++LAGERADP+T+ WA+  L VPVIDHWWQTETGW + ANPLG+E LP K GSP+VPMP
Sbjct: 364 ALFLAGERADPETLKWAERMLDVPVIDHWWQTETGWPMVANPLGLEVLPVKHGSPSVPMP 423

Query: 420 GYTVDILDEGGHPVAPGELGAIAVKLPLPPGTLPTLWNAEDRFKKSYLTTFPGYYETGDA 479
           GY++ +LD+ GH VAPG LG + V+LPLPPG LPTLWNA+ RF  +YL  FPGYY+T DA
Sbjct: 424 GYSIHVLDDAGHAVAPGTLGNVVVQLPLPPGCLPTLWNADARFHSAYLAEFPGYYKTADA 483

Query: 480 GMKDEDGYLYIMARTDDVINVAGHRLSTGAMEEVLAGHPDVAECAVIGVSDSLKGQAPVG 539
           G  DEDGYL+IM+RTDD+IN AGHRLSTGAMEEV A HPDVAECAV+G++D LKGQ P G
Sbjct: 484 GYIDEDGYLFIMSRTDDIINCAGHRLSTGAMEEVCATHPDVAECAVVGIADQLKGQVPCG 543

Query: 540 FLCLNAGCDTPHEDVVAQVVKLVREKIGPVAAFKLACVVDRLPKTRSGKILRGTMVNIAD 599
           FL L          + ++VV+L+RE IGPVAAFK A  VDRLPKTRSGKILRGT+  IAD
Sbjct: 544 FLILKKNVSRETSAIESEVVQLIREAIGPVAAFKTAITVDRLPKTRSGKILRGTIQKIAD 603

Query: 600 GTDWKMPATIDDPAILDEITTALQGLGY 627
           G  WKMPATIDDP IL+EI  AL+  G+
Sbjct: 604 GVPWKMPATIDDPVILEEIAEALRKRGF 631


Lambda     K      H
   0.318    0.137    0.428 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1339
Number of extensions: 46
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 629
Length of database: 636
Length adjustment: 38
Effective length of query: 591
Effective length of database: 598
Effective search space:   353418
Effective search space used:   353418
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Steps (tab-delimited)
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Protein sequences (fasta format)
Organisms (tab-delimited)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources