Align 2-dehydro-3-deoxy-L-rhamnonate dehydrogenase (NAD(+)); 2-keto-3-deoxy-L-rhamnonate dehydrogenase; KDRDH; L-KDR dehydrogenase; L-KDR 4-dehydrogenase; EC 1.1.1.401 (characterized)
to candidate WP_008483001.1 B3C1_RS03700 3-oxoacyl-ACP reductase FabG
Query= SwissProt::Q1NEI6 (249 letters) >NCBI__GCF_000299915.1:WP_008483001.1 Length = 241 Score = 124 bits (310), Expect = 2e-33 Identities = 90/240 (37%), Positives = 128/240 (53%), Gaps = 13/240 (5%) Query: 14 IVTGGASGLGKQVAARIIAEGGAVALWDLNG-DALAATQAEIDAT----HVVALDVSDHA 68 +VTG + G+G +A + AEG AV + N DA A QAEI+A ++A DVSD A Sbjct: 5 LVTGASRGIGAAIAKTLAAEGIAVVIHYRNRQDAALAVQAEIEAAGGQASLLAFDVSDRA 64 Query: 69 AVAAAAKDSAAALGKVDILICSAGIT--GATVPVWEFPVDSFQRVIDINLNGLFYCNREV 126 A AA + AA G ++ +AGIT GA + + D + VI NL+G + + V Sbjct: 65 AAKAALEADMAANGPYYGVVVNAGITRDGAFPALTD---DDWDSVIHTNLDGFYNVLKPV 121 Query: 127 V-PFMLENGYGRIVNLASVAGKEGNPNASAYSASKAGVIGFTKSLGKELAGKGVIANALT 185 V P + GRIV LASV+G GN YSASKAG+IG TK+L ELA + + N + Sbjct: 122 VMPMVQGRKGGRIVTLASVSGLIGNRGQVNYSASKAGIIGATKALALELAKRKITVNCVA 181 Query: 186 PATFESPILDQLPQSQVDYMRSKIPMGRLGLVEESAAMVCFMASEECSFTTASTFDTSGG 245 P ES + +L +++ IP+ R G +E A V F+ S++ + T +GG Sbjct: 182 PGLIESDMTAELEHG--EHIVDLIPLRRAGQAQEVADAVAFLCSDKAGYITRQVLAVNGG 239 Lambda K H 0.318 0.132 0.381 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 146 Number of extensions: 8 Number of successful extensions: 3 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 249 Length of database: 241 Length adjustment: 24 Effective length of query: 225 Effective length of database: 217 Effective search space: 48825 Effective search space used: 48825 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory