GapMind for catabolism of small carbon sources

 

D-xylose catabolism in Gallaecimonas xiamenensis 3-C-1

Best path

xylT, xdh, xylC, xad, kdaD, dopDH

Rules

Overview: Xylose degradation in GapMind is based on MetaCyc pathways I via D-xylulose (link), II via xylitol (link), III or V via 2-dehydro-3-deoxy-D-arabinonate (DKDP) dehydratase (link, link), IV via DKDP aldolase (link), as well as another pathway via DKDP dehydrogenase (PMC6336799).

36 steps (17 with candidates)

Or see definitions of steps

Step Description Best candidate 2nd candidate
xylT D-xylose transporter
xdh D-xylose dehydrogenase B3C1_RS10890 B3C1_RS00525
xylC xylonolactonase
xad D-xylonate dehydratase B3C1_RS10895 B3C1_RS00895
kdaD 2-keto-3-deoxy-D-arabinonate dehydratase
dopDH 2,5-dioxopentanonate dehydrogenase B3C1_RS10885 B3C1_RS02820
Alternative steps:
aldA (glycol)aldehyde dehydrogenase B3C1_RS02820 B3C1_RS13500
aldox-large (glycol)aldehyde oxidoreductase, large subunit
aldox-med (glycol)aldehyde oxidoreductase, medium subunit
aldox-small (glycol)aldehyde oxidoreductase, small subunit
araS component of Arabinose, fructose, xylose porter
araT component of Arabinose, fructose, xylose porter
araU component of Arabinose, fructose, xylose porter
araV component of Arabinose, fructose, xylose porter B3C1_RS04305 B3C1_RS03280
DKDP-aldolase 2-dehydro-3-deoxy-D-arabinonate aldolase
DKDP-dehydrog D-2-keto-3-deoxypentoate dehydrogenase B3C1_RS11585 B3C1_RS16220
Echvi_1871 sodium/xylose cotransporter
gal2 galactose/glucose/xylose uniporter
glcB malate synthase B3C1_RS08520
glcP glucose/mannose/xylose:H+ symporter
gtsA xylose ABC transporter, periplasmic substrate-binding component GtsA
gtsB xylose ABC transporter, permease component 1 GtsB
gtsC xylose ABC transporter, permease component 2 GtsC
gtsD xylose ABC transporter, ATPase component GtsD B3C1_RS04305 B3C1_RS03280
gyaR glyoxylate reductase B3C1_RS14495 B3C1_RS14645
HDOP-hydrol 5-hydroxy-2,4-dioxopentanonate hydrolase B3C1_RS10750
xdhA xylitol dehydrogenase B3C1_RS01685 B3C1_RS06390
xylA xylose isomerase
xylB xylulokinase
xylE_Tm ABC transporter for xylose, substrate binding component xylE B3C1_RS10875
xylF ABC transporter for xylose, substrate binding component xylF
xylF_Tm ABC transporter for xylose, permease component xylF B3C1_RS10865 B3C1_RS10860
xylG ABC transporter for xylose, ATP-binding component xylG B3C1_RS10870
xylH ABC transporter for xylose, permease component xylH B3C1_RS10860 B3C1_RS10865
xylK_Tm ABC transporter for xylose, ATP binding component xylK B3C1_RS10870
xyrA xylitol reductase B3C1_RS14365 B3C1_RS09035

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory