Alignments for a candidate for BPHYT_RS34245 in Oscillibacter ruminantium GH1

GapMind for catabolism of small carbon sources

Alignments for a candidate for BPHYT_RS34245 in Oscillibacter ruminantium GH1

Align ABC transporter related; Flags: Precursor (characterized, see rationale)
to candidate WP_040662390.1 ON16_RS11055 sugar ABC transporter ATP-binding protein

Query= uniprot:B2T9V9
         (510 letters)



>NCBI__GCF_000307265.1:WP_040662390.1
          Length = 499

 Score =  264 bits (675), Expect = 5e-75
 Identities = 168/491 (34%), Positives = 257/491 (52%), Gaps = 8/491 (1%)

Query: 14  VVEALEVTKRFGSTAALNDVSIRVMPGESHALVGRNGAGKSTLVSILTGLRKPDTGEVRF 73
           +VE   + K F     L +V   +  GE  AL+G NGAGKSTL+ IL+G+   D+G ++ 
Sbjct: 5   IVEMENINKSFSGVPVLKNVRFTLKAGEVMALLGENGAGKSTLMKILSGVYVRDSGTMKI 64

Query: 74  SGAAAPSIADRDAWRERVACVYQHSTIIRDLSVAENLFINRQPLRGGVIDWQAMRRDARA 133
            G     +  + A    VA ++Q   + R L+VAEN+F+ R+  +G  ++ + M +  + 
Sbjct: 65  FGEEQGDLDTKKAQELGVAIIHQELNMCRHLTVAENIFLGREVRKGVTLNNREMVKRTQE 124

Query: 134 LLDHWKIDVREDARAGDLSVEARQLVEIARALSYGARFIILDEPTAQLDGDEIKRLFRRI 193
           +LD  KID+  +   GDL V  +Q+VEIA+ALS  A+ +I+DEPT+ L   EI  LFR +
Sbjct: 125 VLDSLKIDLSPNTVVGDLPVSKQQMVEIAKALSVHAKILIMDEPTSALSSKEIDELFRLV 184

Query: 194 SELQREGVTFLFISHHLQEVYEICQAVTVLRDARHIVSAPVSALPREQLIEAMTGERGGL 253
            +L+ EG   ++ISH ++E+  +   VTV+RD   I+    +    +QLI  M G     
Sbjct: 185 DQLRSEGCGIVYISHRMEELSHVANRVTVMRDGEFIMEGNFADYTMDQLIAKMVGR---- 240

Query: 254 AVADAAARGALPADTAVALELKEL-TGADYEGVSFTVKRGEVVGLTGATSSGRTSVAEAI 312
            + +   R        + LE+K L  G     VSF +  GE+VG+ G   +GRT    AI
Sbjct: 241 DIKEKFPRVQCQRGEKI-LEVKNLNAGRMVRDVSFDLYAGEIVGIAGLVGAGRTETTRAI 299

Query: 313 AGLRAAKRGTISVDGAILPPGDVPASLAHGIGCVPKDRHHEGLVLTQSVAENASM-TIAR 371
            G+     G I +DG  +       ++  GI   P+DR  +GL     V EN S+  +  
Sbjct: 300 FGIDPKDSGEIVLDGKSVSIKKPIDAIKAGIVLTPEDRKRDGLCTKLGVRENLSIPNLDW 359

Query: 372 VLGKFGIAAPAKKNAFGQKMIDALGIVAQGPEHVVSGLSGGNQQKVVMARALATNPNVLV 431
           +    G     K+     K+++ L I     E     LSGGNQQKVV+ + LA N  V++
Sbjct: 360 LSNGIGKVNQKKEREIADKVVEELQIKLSSVEINAESLSGGNQQKVVIGKWLARNSRVIM 419

Query: 432 LIDPTAGVDVKSKEALLSVVDRVREEGKAVLVVSGELDD-LRTCDRVLVMFRGRVAAEFP 490
             +PT GVDV +K  + ++++R++++G  VL VS EL + L   DR+LVM  G +  E P
Sbjct: 420 FDEPTRGVDVGAKVEIYNLMNRLKQQGTGVLFVSSELPEILGISDRILVMCDGHLTGELP 479

Query: 491 AGWQDHDLIAS 501
                 D I S
Sbjct: 480 VAEATEDKILS 490



 Score = 66.2 bits (160), Expect = 3e-15
 Identities = 59/237 (24%), Positives = 106/237 (44%), Gaps = 8/237 (3%)

Query: 18  LEVTKRFGSTAALNDVSIRVMPGESHALVGRNGAGKSTLVSILTGLRKPDTGEVRFSGAA 77
           LEV K   +   + DVS  +  GE   + G  GAG++     + G+   D+GE+   G +
Sbjct: 258 LEV-KNLNAGRMVRDVSFDLYAGEIVGIAGLVGAGRTETTRAIFGIDPKDSGEIVLDGKS 316

Query: 78  APSIADRDAWRERVACVYQ---HSTIIRDLSVAENLFI-NRQPLRGGV--IDWQAMRRDA 131
                  DA +  +    +      +   L V ENL I N   L  G+  ++ +  R  A
Sbjct: 317 VSIKKPIDAIKAGIVLTPEDRKRDGLCTKLGVRENLSIPNLDWLSNGIGKVNQKKEREIA 376

Query: 132 RALLDHWKIDVRE-DARAGDLSVEARQLVEIARALSYGARFIILDEPTAQLDGDEIKRLF 190
             +++  +I +   +  A  LS   +Q V I + L+  +R I+ DEPT  +D      ++
Sbjct: 377 DKVVEELQIKLSSVEINAESLSGGNQQKVVIGKWLARNSRVIMFDEPTRGVDVGAKVEIY 436

Query: 191 RRISELQREGVTFLFISHHLQEVYEICQAVTVLRDARHIVSAPVSALPREQLIEAMT 247
             ++ L+++G   LF+S  L E+  I   + V+ D       PV+    ++++   T
Sbjct: 437 NLMNRLKQQGTGVLFVSSELPEILGISDRILVMCDGHLTGELPVAEATEDKILSYAT 493


Lambda     K      H
   0.318    0.134    0.377 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 560
Number of extensions: 29
Number of successful extensions: 9
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 510
Length of database: 499
Length adjustment: 34
Effective length of query: 476
Effective length of database: 465
Effective search space:   221340
Effective search space used:   221340
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources