GapMind for catabolism of small carbon sources

 

Alignments for a candidate for kdgA in Cronobacter condimenti 1330

Align 2-dehydro-3-deoxy-phosphogluconate aldolase (EC 4.1.2.14); 4-Hydroxy-2-oxoglutarate aldolase (EC 4.1.3.16); (4S)-4-hydroxy-2-oxoglutarate aldolase (EC 4.1.3.42) (characterized)
to candidate WP_007677914.1 BN137_RS04830 bifunctional 4-hydroxy-2-oxoglutarate aldolase/2-dehydro-3-deoxy-phosphogluconate aldolase

Query= BRENDA::P0A955
         (213 letters)



>NCBI__GCF_000319285.1:WP_007677914.1
          Length = 212

 Score =  387 bits (994), Expect = e-113
 Identities = 194/212 (91%), Positives = 205/212 (96%)

Query: 1   MKNWKTSAESILTTGPVVPVIVVKKLEHAVPMAKALVAGGVRVLEVTLRTECAVDAIRAI 60
           MKNWKTSAE IL +GPVVPVIV+ KLEHAVPMAKAL+AGGVRVLEVTLRT CA+DAIRAI
Sbjct: 1   MKNWKTSAEQILKSGPVVPVIVINKLEHAVPMAKALLAGGVRVLEVTLRTPCAMDAIRAI 60

Query: 61  AKEVPEAIVGAGTVLNPQQLAEVTEAGAQFAISPGLTEPLLKAATEGTIPLIPGISTVSE 120
           AKEVP+AI+GAGTVLN +QL EVTEAGAQFAISPGLTEPLLKAATEG+IPLIPGISTVSE
Sbjct: 61  AKEVPDAIIGAGTVLNAEQLKEVTEAGAQFAISPGLTEPLLKAATEGSIPLIPGISTVSE 120

Query: 121 LMLGMDYGLKEFKFFPAEANGGVKALQAIAGPFSQVRFCPTGGISPANYRDYLALKSVLC 180
           LMLGMDYGLKEFKFFPAEANGG KALQAIAGPFSQVRFCPTGGISPANYRDYLALKSVLC
Sbjct: 121 LMLGMDYGLKEFKFFPAEANGGTKALQAIAGPFSQVRFCPTGGISPANYRDYLALKSVLC 180

Query: 181 IGGSWLVPADALEAGDYDRITKLAREAVEGAK 212
           IGGSWLVPADA+EAGD+DRITKLAREAVEGA+
Sbjct: 181 IGGSWLVPADAMEAGDWDRITKLAREAVEGAQ 212


Lambda     K      H
   0.317    0.135    0.387 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 295
Number of extensions: 5
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 213
Length of database: 212
Length adjustment: 21
Effective length of query: 192
Effective length of database: 191
Effective search space:    36672
Effective search space used:    36672
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 45 (21.9 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory