GapMind for catabolism of small carbon sources

 

Finding step HSERO_RS03640 for D-mannose catabolism in Cronobacter condimenti 1330

4 candidates for HSERO_RS03640: mannose ABC transporter, ATPase component

Score Gene Description Similar to Id. Cov. Bits Other hit Other id. Other bits
med BN137_RS17460 ribose ABC transporter ATP-binding protein RbsA Ribose import ATP-binding protein RbsA; EC 7.5.2.7 (characterized, see rationale) 45% 95% 419.5 ribose transport, ATP-binding protein RbsA; EC 3.6.3.17 91% 887.1
med BN137_RS01915 xylose ABC transporter ATP-binding protein Ribose import ATP-binding protein RbsA; EC 7.5.2.7 (characterized, see rationale) 43% 96% 388.7 Xylose import ATP-binding protein XylG; EC 7.5.2.10 84% 855.9
med BN137_RS14410 sugar ABC transporter ATP-binding protein Ribose import ATP-binding protein RbsA; EC 7.5.2.7 (characterized, see rationale) 42% 95% 384 RhaT, component of Rhamnose porter (Richardson et al., 2004) (Transport activity is dependent on rhamnokinase (RhaK; AAQ92412) activity (Richardson and Oresnik, 2007) This could be an example of group translocation!) 56% 538.5
lo BN137_RS06860 galactose/methyl galactoside ABC transporter ATP-binding protein MglA Ribose import ATP-binding protein RbsA; EC 7.5.2.7 (characterized, see rationale) 40% 95% 385.2 Galactose/methyl galactoside import ATP-binding protein MglA aka B2149, component of Galactose/glucose (methyl galactoside) porter 94% 944.9

Confidence: high confidence medium confidence low confidence
transporter – transporters and PTS systems are shaded because predicting their specificity is particularly challenging.

GapMind searches the predicted proteins for candidates by using ublast (a fast alternative to protein BLAST) to find similarities to characterized proteins or by using HMMer to find similarities to enzyme models (usually from TIGRFams). For alignments to characterized proteins (from ublast), scores of 44 bits correspond to an expectation value (E) of about 0.001.

Definition of step HSERO_RS03640

Or cluster all characterized HSERO_RS03640 proteins

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory