GapMind for catabolism of small carbon sources

 

Alignments for a candidate for mglB in Cronobacter condimenti 1330

Align glucose transporter, periplasmic substrate-binding component (characterized)
to candidate WP_007680677.1 BN137_RS01920 D-xylose ABC transporter substrate-binding protein

Query= reanno::Phaeo:GFF3639
         (341 letters)



>NCBI__GCF_000319285.1:WP_007680677.1
          Length = 330

 Score =  233 bits (594), Expect = 5e-66
 Identities = 127/318 (39%), Positives = 191/318 (60%), Gaps = 7/318 (2%)

Query: 15  TASMAFAEDVTVGVSWSNFQEERWKTDEAAIKAALEAKGATYVSADAQSSSAKQLSDIES 74
           T+    A+DV +G++  + + ERW+ D     +  E+ GA      A  +   Q+S IE+
Sbjct: 17  TSFSGIAKDVKIGMAIDDLRLERWQKDRDIFVSKAESLGAKVFVQSANGNEETQMSQIEN 76

Query: 75  LIAQGVDALIVLAQDAQAIGPAVQAAADEGIPVVAYDRLIEDGRA-FYLTFDNVEVGRMQ 133
           +I +GVD L+++  + Q +   +  A  EGI V+AYDR+I +    FY++FDN +VG +Q
Sbjct: 77  MINRGVDVLVIIPYNGQVLSNVIAEAKREGIKVLAYDRMINNADIDFYISFDNEKVGEIQ 136

Query: 134 ARAVLEAQPSGNYVMIKGSPTDPNADFLRGGQQEIIQAAIDSGDIKIVGEAYTDGWLPAN 193
           A+++++  P GNY ++ GSP D NA   R GQ ++++  IDSG IK+VG+ + DGWLP N
Sbjct: 137 AQSLVDNVPQGNYFLMGGSPVDNNARLFRDGQMKVLKPFIDSGKIKVVGDQWVDGWLPEN 196

Query: 194 AQRNMEQILTANDNKVDAVVASNDGTAGGVVAALTAQGMEG-IAVSGQDGDHAALNRVAK 252
           A + ME  LTAN+NK+DAVVASND TAGG + AL+AQG+   +A+SGQD D A + R+  
Sbjct: 197 ALKIMENALTANNNKIDAVVASNDATAGGAIQALSAQGLASKVAISGQDADLAGIKRIMA 256

Query: 253 GTQTVSVWKDARDLGKAAANIAVEMAEGAVMGDVAGGAAWTSPAGTELTARFLEPIPVTA 312
           GTQT++V+K    L   AA +AVE+             A  +    ++ +R L PI V  
Sbjct: 257 GTQTMTVYKPITQLANTAAEVAVELGN----DKQPKSDATLNNGLKDVPSRLLTPIKVDK 312

Query: 313 DNL-SVVVDAGWITKEAL 329
            N+ S V+  G+  K  L
Sbjct: 313 SNIESTVIKDGFHKKSDL 330


Lambda     K      H
   0.313    0.128    0.362 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 232
Number of extensions: 9
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 341
Length of database: 330
Length adjustment: 28
Effective length of query: 313
Effective length of database: 302
Effective search space:    94526
Effective search space used:    94526
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.2 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 49 (23.5 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory