GapMind for catabolism of small carbon sources

 

Protein WP_017549192.1 in Salinicoccus carnicancri Crm

Annotation: NCBI__GCF_000330705.1:WP_017549192.1

Length: 459 amino acids

Source: GCF_000330705.1 in NCBI

Candidate for 19 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-arginine catabolism gabD hi Succinate-semialdehyde dehydrogenase [NADP(+)]; SSDH; EC 1.2.1.79 (characterized) 59% 100% 568.5 3-sulfolactaldehyde dehydrogenase; SLA dehydrogenase; EC 1.2.1.97 49% 449.1
L-citrulline catabolism gabD hi Succinate-semialdehyde dehydrogenase [NADP(+)]; SSDH; EC 1.2.1.79 (characterized) 59% 100% 568.5 3-sulfolactaldehyde dehydrogenase; SLA dehydrogenase; EC 1.2.1.97 49% 449.1
putrescine catabolism gabD hi Succinate-semialdehyde dehydrogenase [NADP(+)]; SSDH; EC 1.2.1.79 (characterized) 59% 100% 568.5 3-sulfolactaldehyde dehydrogenase; SLA dehydrogenase; EC 1.2.1.97 49% 449.1
L-arginine catabolism davD med glutarate-semialdehyde dehydrogenase (EC 1.2.1.20) (characterized) 49% 95% 448.7 Succinate-semialdehyde dehydrogenase [NADP(+)]; SSDH; EC 1.2.1.79 59% 568.5
L-citrulline catabolism davD med glutarate-semialdehyde dehydrogenase (EC 1.2.1.20) (characterized) 49% 95% 448.7 Succinate-semialdehyde dehydrogenase [NADP(+)]; SSDH; EC 1.2.1.79 59% 568.5
L-lysine catabolism davD med glutarate-semialdehyde dehydrogenase (EC 1.2.1.20) (characterized) 49% 95% 448.7 Succinate-semialdehyde dehydrogenase [NADP(+)]; SSDH; EC 1.2.1.79 59% 568.5
L-proline catabolism davD med glutarate-semialdehyde dehydrogenase (EC 1.2.1.20) (characterized) 49% 95% 448.7 Succinate-semialdehyde dehydrogenase [NADP(+)]; SSDH; EC 1.2.1.79 59% 568.5
4-hydroxybenzoate catabolism adh med aldehyde dehydrogenase (NAD+) (EC 1.2.1.3) (characterized) 46% 86% 408.7 Succinate-semialdehyde dehydrogenase [NADP(+)]; SSDH; EC 1.2.1.79 59% 568.5
2'-deoxyinosine catabolism adh med aldehyde dehydrogenase (NAD+) (EC 1.2.1.3) (characterized) 46% 86% 408.7 Succinate-semialdehyde dehydrogenase [NADP(+)]; SSDH; EC 1.2.1.79 59% 568.5
2-deoxy-D-ribose catabolism adh med aldehyde dehydrogenase (NAD+) (EC 1.2.1.3) (characterized) 46% 86% 408.7 Succinate-semialdehyde dehydrogenase [NADP(+)]; SSDH; EC 1.2.1.79 59% 568.5
ethanol catabolism adh med aldehyde dehydrogenase (NAD+) (EC 1.2.1.3) (characterized) 46% 86% 408.7 Succinate-semialdehyde dehydrogenase [NADP(+)]; SSDH; EC 1.2.1.79 59% 568.5
L-threonine catabolism adh med aldehyde dehydrogenase (NAD+) (EC 1.2.1.3) (characterized) 46% 86% 408.7 Succinate-semialdehyde dehydrogenase [NADP(+)]; SSDH; EC 1.2.1.79 59% 568.5
thymidine catabolism adh med aldehyde dehydrogenase (NAD+) (EC 1.2.1.3) (characterized) 46% 86% 408.7 Succinate-semialdehyde dehydrogenase [NADP(+)]; SSDH; EC 1.2.1.79 59% 568.5
L-tryptophan catabolism adh med aldehyde dehydrogenase (NAD+) (EC 1.2.1.3) (characterized) 46% 86% 408.7 Succinate-semialdehyde dehydrogenase [NADP(+)]; SSDH; EC 1.2.1.79 59% 568.5
L-arabinose catabolism xacF med Alpha-ketoglutaric semialdehyde dehydrogenase 1; alphaKGSA dehydrogenase 1; 2,5-dioxovalerate dehydrogenase 1; 2-oxoglutarate semialdehyde dehydrogenase 1; KGSADH-I; Succinate-semialdehyde dehydrogenase [NAD(+)]; SSDH; EC 1.2.1.26; EC 1.2.1.24 (characterized) 43% 95% 363.2 Succinate-semialdehyde dehydrogenase [NADP(+)]; SSDH; EC 1.2.1.79 59% 568.5
D-galacturonate catabolism dopDH med Alpha-ketoglutaric semialdehyde dehydrogenase 1; alphaKGSA dehydrogenase 1; 2,5-dioxovalerate dehydrogenase 1; 2-oxoglutarate semialdehyde dehydrogenase 1; KGSADH-I; Succinate-semialdehyde dehydrogenase [NAD(+)]; SSDH; EC 1.2.1.26; EC 1.2.1.24 (characterized) 43% 95% 363.2 Succinate-semialdehyde dehydrogenase [NADP(+)]; SSDH; EC 1.2.1.79 59% 568.5
D-glucuronate catabolism dopDH med Alpha-ketoglutaric semialdehyde dehydrogenase 1; alphaKGSA dehydrogenase 1; 2,5-dioxovalerate dehydrogenase 1; 2-oxoglutarate semialdehyde dehydrogenase 1; KGSADH-I; Succinate-semialdehyde dehydrogenase [NAD(+)]; SSDH; EC 1.2.1.26; EC 1.2.1.24 (characterized) 43% 95% 363.2 Succinate-semialdehyde dehydrogenase [NADP(+)]; SSDH; EC 1.2.1.79 59% 568.5
D-xylose catabolism dopDH med Alpha-ketoglutaric semialdehyde dehydrogenase 1; alphaKGSA dehydrogenase 1; 2,5-dioxovalerate dehydrogenase 1; 2-oxoglutarate semialdehyde dehydrogenase 1; KGSADH-I; Succinate-semialdehyde dehydrogenase [NAD(+)]; SSDH; EC 1.2.1.26; EC 1.2.1.24 (characterized) 43% 95% 363.2 Succinate-semialdehyde dehydrogenase [NADP(+)]; SSDH; EC 1.2.1.79 59% 568.5
L-lysine catabolism amaB lo Δ1-piperideine-6-carboxylate dehydrogenase (characterized) 34% 78% 223 Succinate-semialdehyde dehydrogenase [NADP(+)]; SSDH; EC 1.2.1.79 59% 568.5

Sequence Analysis Tools

View WP_017549192.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

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Sequence

MSEEIAVMNPATGEEIRSIKKDSREDIEAKIETAHQAFKKYKKKNAHDRSALLSEWAKLI
RENKDEIAQLITKENGKPLAESLGEVEYACSYIDYYAEEGKRVYGRTIPPHEDDVRLVVT
KQPIGVVAAITPWNFPVAMMARKAAPAVAAGCTFLVKPARETPLSAMRFLDLAKDAGFEA
GVIQYVNASGKDAGEVFSQSDKVMKLTFTGSTAVGKKLMEGAASTVKNITMELGGHAPLV
VHKDADLETAVDHAIATKFRNSGQTCVCANRFYVHEDIADAFSEKFSEKVKKLKMGNGMD
EGVQVGPMINKDGYDKVMEHINDARQKGGTITAGGNGNNSEGYFIEPTVITDATDEMIIM
NEETFGPVAPIQTFSNLDEAIEKANDTEYGLAAYFFTESYRDGIRLQEGLNFGVLGWNNG
KPSAAHIPFGGLKESGIGREGGLEGIEPYLETKITSMKI

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory