Align Putative phosphotransferase enzyme IIA component YpqE (characterized, see rationale)
to candidate WP_017548976.1 C792_RS0108145 PTS glucose transporter subunit IIA
Query= uniprot:P50829 (168 letters) >NCBI__GCF_000330705.1:WP_017548976.1 Length = 167 Score = 162 bits (411), Expect = 2e-45 Identities = 75/160 (46%), Positives = 113/160 (70%), Gaps = 1/160 (0%) Query: 1 MLKKLFGMGKIQEKVTEEVIYSPADGTVMDLSDVPDPVFSQKMMGEGIAVEPSSGEIVSP 60 M K LFG + T E I SP +G+ + L D+PDPVF++KMMGEG ++PS GE+V+P Sbjct: 1 MFKNLFGKKEKDVDKTLE-IKSPMNGSYVPLEDIPDPVFAEKMMGEGFGIDPSHGEVVAP 59 Query: 61 AEGEVIQIFHTKHAVGIRTRSGIELLIHVGLETVNMNGEGFTAHIKEGDKVKVGDPLITC 120 +G ++Q+F T HA+GI+T +G+E+LIH+GLETV M G+GF + EGD+++VGD L+T Sbjct: 60 VDGVIMQVFPTNHAIGIKTDNGVEVLIHIGLETVAMEGKGFEGFVSEGDRIEVGDKLVTF 119 Query: 121 DLELIKEKASSTVIPIVIMNGEAVGSMVSAGEKAARKGES 160 D++L+KE+A+ST+ P++I N + + S KGE+ Sbjct: 120 DIDLVKEEANSTISPVIITNSDELASFDIQSVSDTVKGET 159 Lambda K H 0.314 0.134 0.365 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 141 Number of extensions: 4 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 168 Length of database: 167 Length adjustment: 18 Effective length of query: 150 Effective length of database: 149 Effective search space: 22350 Effective search space used: 22350 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.2 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 42 (21.9 bits) S2: 43 (21.2 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory