Align HutV aka HISV aka R02702 aka SMC00670, component of Uptake system for hisitidine, proline, proline-betaine and glycine-betaine (characterized)
to candidate WP_017547921.1 C792_RS0102795 methionine ABC transporter ATP-binding protein
Query= TCDB::Q9KKE1 (275 letters) >NCBI__GCF_000330705.1:WP_017547921.1 Length = 340 Score = 167 bits (424), Expect = 2e-46 Identities = 98/262 (37%), Positives = 154/262 (58%), Gaps = 21/262 (8%) Query: 4 IEIRNVYKIFGHDAKKALTMVEDGLDKADILSRSGCTVGLNDVSLKIGAGKIFVIMGLSG 63 I+IRNV KIF ++S ++DVS+ + G+IF ++G SG Sbjct: 2 IDIRNVTKIFK--------------------TKSTDIRAVDDVSMNVTQGEIFGVIGYSG 41 Query: 64 SGKSTLVRHINRLIEPTSGEVLFDGDNILDLGAKALRAFRMRRVSMVFQSFALMPHRTVL 123 +GKSTL+R N L P+SG+V+ D I + ALR R ++VSM+FQ F L+ RTV Sbjct: 42 AGKSTLIRLFNGLEMPSSGDVVIGADVISSMSKPALRKKR-QKVSMIFQHFNLLWSRTVA 100 Query: 124 QNVVYGQRVRGVSKDDAREIGMKWIDTVGLSGYDAKFPHQLSGGMKQRVGLARALAADTD 183 +N+ + + GV K + ++ + +D VGL+G + +P QLSGG KQRVG+ARAL+ + + Sbjct: 101 ENISFPLEIAGVPKKERQKKVKELVDLVGLAGREDNYPSQLSGGQKQRVGIARALSNEPN 160 Query: 184 VILMDEAFSALDPLIRGDMQDQLLQLQRNLAKTIVFITHDLDEALRIGSEIAILRDGQVV 243 V+L DEA SALDP ++ D L++++ + TIV ITH++ +I A++ +G++V Sbjct: 161 VLLCDEATSALDPETTDEVLDLLVKIRDEMNLTIVLITHEMHVIRKICDRAAVMENGRIV 220 Query: 244 QVGTPNDILDNPANDYVARFVQ 265 + G + NP + RFV+ Sbjct: 221 ETGPVLKLFQNPEHSVTKRFVK 242 Lambda K H 0.323 0.139 0.397 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 233 Number of extensions: 6 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 275 Length of database: 340 Length adjustment: 27 Effective length of query: 248 Effective length of database: 313 Effective search space: 77624 Effective search space used: 77624 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.4 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.9 bits) S2: 48 (23.1 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory