Align SDR family oxidoreductase (characterized, see rationale)
to candidate WP_017548686.1 C792_RS0106680 glucose 1-dehydrogenase
Query= uniprot:A0A4P7ABK7 (254 letters) >NCBI__GCF_000330705.1:WP_017548686.1 Length = 243 Score = 134 bits (338), Expect = 1e-36 Identities = 88/252 (34%), Positives = 138/252 (54%), Gaps = 20/252 (7%) Query: 6 GRLAGKTVLITAAAQGIGRASTELFAREGARVIATDISKTHLEELASIAGVETHLL--DV 63 GR+ GK V++T G+G + + F EGA+V+ TD+ + L G ++ + DV Sbjct: 2 GRVNGKVVIVTGGTGGMGASHVKKFVSEGAQVVFTDLDEGAGRVLQDEMGPQSLFIKQDV 61 Query: 64 TDDDAIKALVAK----VGTVDVLFNCAGYVAAGNILECDDKAWDFSFNLNAKAMFHTIRA 119 TD ++ ++ + +G V+VL N AG + + +D +N KA+F ++A Sbjct: 62 TDSESWNKVIVETEKNLGPVNVLVNNAGVNYSEETEAFPEDEYDRVIEVNQKAVFLGMKA 121 Query: 120 VLPGMLAKKAGSIVNIASAASSVKGVANRFAYGASKAAVVGLTKSVAADFVSQGIRCNAI 179 V+P M +GSI+NI+S ++G AY ASK AV G+TK A + GIR N++ Sbjct: 122 VIPSMKKAASGSIINISSIGG-IRGRMESVAYSASKFAVTGMTKVGALELGKYGIRVNSV 180 Query: 180 CPGTIESPSLNQRISTQAKETGKSEDEVRAAFVARQPMGRIGKAEEVAALALYLASDESN 239 P + + ++ ST K A + +P+GR+ EEV+AL LYLASDES+ Sbjct: 181 HPSLVNTGLFSE--STGGK-----------AVASDRPLGRVLTKEEVSALVLYLASDESS 227 Query: 240 FTTGSIHMIDGG 251 +TTG+ H+IDGG Sbjct: 228 YTTGAEHVIDGG 239 Lambda K H 0.316 0.129 0.359 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 158 Number of extensions: 12 Number of successful extensions: 4 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 254 Length of database: 243 Length adjustment: 24 Effective length of query: 230 Effective length of database: 219 Effective search space: 50370 Effective search space used: 50370 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory