Alignments for a candidate for cycA in Streptococcus massiliensis 4401825

GapMind for catabolism of small carbon sources

Alignments for a candidate for cycA in Streptococcus massiliensis 4401825

Align Serine transporter, SerP2 or YdgB, of 459 aas and 12 TMSs (Trip et al. 2013). Transports L-alanine (Km = 20 μM), D-alanine (Km = 38 μM), L-serine, D-serine (Km = 356 μM) and glycine (Noens and Lolkema 2015). The encoding gene is adjacent to the one encoding SerP1 (TC# 2.A.3.1.21) (characterized)
to candidate WP_018371759.1 BN415_RS05555 amino acid permease

Query= TCDB::F2HQ24
         (457 letters)



>NCBI__GCF_000341525.1:WP_018371759.1
          Length = 459

 Score =  461 bits (1186), Expect = e-134
 Identities = 222/450 (49%), Positives = 317/450 (70%), Gaps = 2/450 (0%)

Query: 4   NQNEENKPSQRGLKNRHIQLIAIAGTIGTGLFLGAGKSIHLTGPSIIFVYLIIGALMYIL 63
           N++      +RGLKNRH+QLIAIAGTIGTGLFLGAG++I+LTGPSI+ VY + G  MY++
Sbjct: 5   NKHPHKNGLKRGLKNRHVQLIAIAGTIGTGLFLGAGRTINLTGPSILLVYTLTGGFMYLM 64

Query: 64  LRAIGEMLYQDPNQHSFLNFVSRYLGEKPGYFIQWSYLLVVVFVAMAELIAIGTYINFWL 123
           +RAIGEMLY DP+QH+F+NF+++YLG   GYF  WSY + ++ + MA++ A+  Y+ +W 
Sbjct: 65  MRAIGEMLYYDPDQHTFINFITKYLGNGWGYFSGWSYWISLICIGMADITAVAKYVQYWF 124

Query: 124 PDLPIWMTEVFVLVLLTLLNTLNPKFFGETEFWFGMIKIVAIIGLILTAIILIFSHYHTG 183
           P  P W  ++  L +L+ +N +  + FGE EFWF MIKIVAI+ LI TAI +  + + T 
Sbjct: 125 PTWPSWAIQLVFLAILSSVNLIAVRIFGEVEFWFAMIKIVAILALIATAIFMALTGFETP 184

Query: 184 TDTVSVTNITKGFEFFPNGLSNFFESFQMVMFAFVSMEFIGMTAAETDNPRPTLKKAINQ 243
              V + NI   F  FPNG  +F  +FQMV FA+ ++EFIG+T +ET NPR  L KAI +
Sbjct: 185 HGAVGLHNIFDNFSLFPNGWVSFVMAFQMVFFAYQAIEFIGITTSETANPRQVLPKAIKE 244

Query: 244 IPIRIVLFYVGALLAIMSIYQWRDIPADKSPFVTIFQLIGIKWAAALVNFVVLTSAASAL 303
           IP+RIVLFY G+LLAIM+I  WR + A +SPFVT+FQL G+KWAAAL+NFVVLTSAASAL
Sbjct: 245 IPLRIVLFYGGSLLAIMTIIPWRQLSATESPFVTVFQLAGVKWAAALINFVVLTSAASAL 304

Query: 304 NSALFSITRNLYSLSKLNNDKILKPF--TKFSKAGVPVNALLFTSLLILFTPFISMIPAI 361
           NSAL+S  R+LY ++    +K+L      K S+  VP NA+L +   I     I+++P +
Sbjct: 305 NSALYSTGRHLYQIALETPNKLLLKLGANKLSRQHVPQNAILVSVATIALAALINVLPGV 364

Query: 362 SNSFVFITSVATNLFLVVYLMTLITYLKYRKSSDFDPKGFVLPAAHIFIPLAIAGFVLIF 421
           S+SF  I + ++ +++ +Y +T+I +L+YRKS DF   G+++PA  I  PL +  F  +F
Sbjct: 365 SDSFSLIAASSSGVYIAIYTLTMIAHLRYRKSEDFLSDGYLMPAYKILNPLTMVFFSFVF 424

Query: 422 ISLFCFKDTIVPAIGSVIWVLIFGLFTFFK 451
           +++F  + T+  AIGSVIW++I GL++ +K
Sbjct: 425 VTMFLQESTLKGAIGSVIWIVILGLYSQYK 454


Lambda     K      H
   0.330    0.144    0.431 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 595
Number of extensions: 24
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 457
Length of database: 459
Length adjustment: 33
Effective length of query: 424
Effective length of database: 426
Effective search space:   180624
Effective search space used:   180624
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources