GapMind for catabolism of small carbon sources

 

Protein WP_017867756.1 in Lactobacillus pobuzihii E100301

Annotation: NCBI__GCF_000349725.1:WP_017867756.1

Length: 455 amino acids

Source: GCF_000349725.1 in NCBI

Candidate for 24 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
L-arginine catabolism gabD hi succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79) (characterized) 46% 96% 397.1
L-citrulline catabolism gabD hi succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79) (characterized) 46% 96% 397.1
putrescine catabolism gabD hi succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79) (characterized) 46% 96% 397.1
4-hydroxybenzoate catabolism adh lo Aldehyde dehydrogenase; NAD/NADP-dependent aldehyde dehydrogenase; EC 1.2.1.3; EC 1.2.1.4 (characterized) 38% 99% 313.9 succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79) 46% 397.1
2'-deoxyinosine catabolism adh lo Aldehyde dehydrogenase; NAD/NADP-dependent aldehyde dehydrogenase; EC 1.2.1.3; EC 1.2.1.4 (characterized) 38% 99% 313.9 succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79) 46% 397.1
2-deoxy-D-ribose catabolism adh lo Aldehyde dehydrogenase; NAD/NADP-dependent aldehyde dehydrogenase; EC 1.2.1.3; EC 1.2.1.4 (characterized) 38% 99% 313.9 succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79) 46% 397.1
ethanol catabolism adh lo Aldehyde dehydrogenase; NAD/NADP-dependent aldehyde dehydrogenase; EC 1.2.1.3; EC 1.2.1.4 (characterized) 38% 99% 313.9 succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79) 46% 397.1
L-threonine catabolism adh lo Aldehyde dehydrogenase; NAD/NADP-dependent aldehyde dehydrogenase; EC 1.2.1.3; EC 1.2.1.4 (characterized) 38% 99% 313.9 succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79) 46% 397.1
thymidine catabolism adh lo Aldehyde dehydrogenase; NAD/NADP-dependent aldehyde dehydrogenase; EC 1.2.1.3; EC 1.2.1.4 (characterized) 38% 99% 313.9 succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79) 46% 397.1
L-tryptophan catabolism adh lo Aldehyde dehydrogenase; NAD/NADP-dependent aldehyde dehydrogenase; EC 1.2.1.3; EC 1.2.1.4 (characterized) 38% 99% 313.9 succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79) 46% 397.1
L-arginine catabolism davD lo Glutarate-semialdehyde dehydrogenase; EC 1.2.1.- (characterized) 30% 92% 231.1 succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79) 46% 397.1
L-citrulline catabolism davD lo Glutarate-semialdehyde dehydrogenase; EC 1.2.1.- (characterized) 30% 92% 231.1 succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79) 46% 397.1
L-lysine catabolism davD lo Glutarate-semialdehyde dehydrogenase; EC 1.2.1.- (characterized) 30% 92% 231.1 succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79) 46% 397.1
L-proline catabolism davD lo Glutarate-semialdehyde dehydrogenase; EC 1.2.1.- (characterized) 30% 92% 231.1 succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79) 46% 397.1
L-fucose catabolism aldA lo lactaldehyde dehydrogenase (EC 1.2.1.22) (characterized) 31% 96% 214.5 succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79) 46% 397.1
L-rhamnose catabolism aldA lo lactaldehyde dehydrogenase (EC 1.2.1.22) (characterized) 31% 96% 214.5 succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79) 46% 397.1
L-threonine catabolism aldA lo lactaldehyde dehydrogenase (EC 1.2.1.22) (characterized) 31% 96% 214.5 succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79) 46% 397.1
L-phenylalanine catabolism pad-dh lo Phenylacetaldehyde dehydrogenase; PAD; EC 1.2.1.39 (characterized) 30% 91% 205.7 succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79) 46% 397.1
L-arabinose catabolism xacF lo Alpha-ketoglutaric semialdehyde dehydrogenase 1; alphaKGSA dehydrogenase 1; 2,5-dioxovalerate dehydrogenase 1; 2-oxoglutarate semialdehyde dehydrogenase 1; KGSADH-I; Succinate-semialdehyde dehydrogenase [NAD(+)]; SSDH; EC 1.2.1.26; EC 1.2.1.24 (characterized) 30% 90% 200.3 succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79) 46% 397.1
D-galacturonate catabolism dopDH lo Alpha-ketoglutaric semialdehyde dehydrogenase 1; alphaKGSA dehydrogenase 1; 2,5-dioxovalerate dehydrogenase 1; 2-oxoglutarate semialdehyde dehydrogenase 1; KGSADH-I; Succinate-semialdehyde dehydrogenase [NAD(+)]; SSDH; EC 1.2.1.26; EC 1.2.1.24 (characterized) 30% 90% 200.3 succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79) 46% 397.1
D-glucuronate catabolism dopDH lo Alpha-ketoglutaric semialdehyde dehydrogenase 1; alphaKGSA dehydrogenase 1; 2,5-dioxovalerate dehydrogenase 1; 2-oxoglutarate semialdehyde dehydrogenase 1; KGSADH-I; Succinate-semialdehyde dehydrogenase [NAD(+)]; SSDH; EC 1.2.1.26; EC 1.2.1.24 (characterized) 30% 90% 200.3 succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79) 46% 397.1
D-xylose catabolism dopDH lo Alpha-ketoglutaric semialdehyde dehydrogenase 1; alphaKGSA dehydrogenase 1; 2,5-dioxovalerate dehydrogenase 1; 2-oxoglutarate semialdehyde dehydrogenase 1; KGSADH-I; Succinate-semialdehyde dehydrogenase [NAD(+)]; SSDH; EC 1.2.1.26; EC 1.2.1.24 (characterized) 30% 90% 200.3 succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79) 46% 397.1
4-hydroxybenzoate catabolism praB lo 2-hydroxymuconate-6-semialdehyde dehydrogenase (EC 1.2.1.85) (characterized) 30% 69% 177.2 succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79) 46% 397.1
L-tryptophan catabolism praB lo 2-hydroxymuconate-6-semialdehyde dehydrogenase (EC 1.2.1.85) (characterized) 30% 69% 177.2 succinate-semialdehyde dehydrogenase (NADP+) (EC 1.2.1.79) 46% 397.1

Sequence Analysis Tools

View WP_017867756.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MSYAAINPFNGQKIRDFDNVTDQQLEVILDQAQGFYEASQKQDVEERTEFLNGLANEFEK
NSEKYARILSTNMGKLLVEARQEVAKTVSFARYYAKNGVDLTQPQPYNDLPNGQAQVEIS
GTGIILAVEPWNFPYAQVMRVLAPNFILGNPVILKHASIVPEAALAFQEACDNADLPLGA
FTNVFANYDQIDHIIEDPRVQGVALTGSEGAGRKIAASAGQNLTKSTMELGGTDVFIVLD
DVDITKAAKDAAKARLTNAGQVCTAAKRYIVSSKIYPDFLDALKEEFAKYQVGDPLDENT
TLAPLSSKDAQQQLQAQVEKVIAGGSEVLYGDLTPNEGDGAGFSPLILSGMEQDNPMYEE
ELFGPVAQIYQAQSDDEIVKIANNSRHGLGGAIYTKDIERGKKIASRIETGQVAINSILS
SQAEVPFGGVKDSGYGRELSDWGIYEFANVKPVMY

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Links

Downloads

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory