Alignments for a candidate for cycA in Lactobacillus pobuzihii E100301

GapMind for catabolism of small carbon sources

Alignments for a candidate for cycA in Lactobacillus pobuzihii E100301

Align General amino-acid permease GAP2 (characterized)
to candidate WP_051056359.1 LACPOB_RS0100170 amino acid permease

Query= SwissProt::A0A1D8PK89
         (588 letters)



>NCBI__GCF_000349725.1:WP_051056359.1
          Length = 470

 Score =  341 bits (875), Expect = 3e-98
 Identities = 184/469 (39%), Positives = 274/469 (58%), Gaps = 16/469 (3%)

Query: 88  MIAIGGSIGTGLFVGSGSSLHTGGPAGLLIAYILIGTMIYCTVMSLGELAVTFPVSGAFV 147
           MIA+GGSIGTGLFV SGS++ T GP G L+AYI IG M+Y  + SLGE+A   PV+G+F 
Sbjct: 1   MIALGGSIGTGLFVASGSAISTAGPGGALVAYIGIGLMVYFLMTSLGEMATYLPVTGSFA 60

Query: 148 TYNSRFIDPSWGFAMAWNYAMQWLVVLPLELVAAAMTVKYWDAKTNSAAFVVIFYVLIVA 207
           TY  RF+DP+ GFAM WNY   W + L +++    + +K+W  +  S AF V    +I  
Sbjct: 61  TYARRFVDPAMGFAMGWNYWFNWAITLAVDVSTVGLVLKFWFPRVPSWAFSVGALAIIFI 120

Query: 208 INFFGVRGYGEAEFIFSAVKVLAVLGFIILGIVLCAGGGPQGGYIGGKNWYIEG-----A 262
           IN   V  +GE E+  + +KV+ V+ F+++G +   G       I G   Y+E      A
Sbjct: 121 INALSVNSFGETEYWMALIKVVTVIVFLVVGFLTIFG------IINGHATYLENFVYRKA 174

Query: 263 PFPNGAKGVITVFVNAAFAFAGTELCGLAAAETENPRKSLPKACKQVFWRITLFYVICLT 322
           PF  G   +++VFV A F+F GTEL G+ A E+ENP +S+PKA KQVFWRI LFY++ + 
Sbjct: 175 PFVGGIPTILSVFVVAGFSFQGTELIGITAGESENPGESIPKAIKQVFWRIILFYILSIF 234

Query: 323 LVGLLVPWNDERLLGSSSADASASPFVISIRNAGIKGLPSVMNVVIMIAVLSVGNSSVYG 382
           ++  L+P+    LLGS + D + SPF +  R AG+ G   +MN VI+ +VLS  NS +Y 
Sbjct: 235 VIAALIPYTSPNLLGSDAGDVTISPFTLVFRRAGLAGAAGIMNAVILTSVLSAANSGMYA 294

Query: 383 SSRTLAALAASNQAPKIFGYIDKQGRPLVGIIAQLLVGLLCFLAASDKQGEVFNWLLALS 442
           S+R L +L  S  APKIF  ++ +G P+  ++    VGL+ F A S     ++N+L++ S
Sbjct: 295 STRMLFSLGVSGDAPKIFKRVNTRGIPMPALVGTAAVGLITF-ATSIFGDRIYNFLVSAS 353

Query: 443 GLSSIFTWGSINVCLIRFRRALAAQGRDTGELVFTSQVGVIGAIWGAFLNTVVLCLQFWI 502
           GLS    W  I +   RFRRA  AQG D  EL + ++    G I    L  +V+  Q   
Sbjct: 354 GLSGFIAWVGIAISHYRFRRAFKAQGHDLSELQYHAKWFPFGPILALILCILVIIGQDLG 413

Query: 503 AVWPLHSSPSAEAFFSAYLTVPVVIVFYVGHKLWTKNWQVYICAKDIDI 551
           +     +S   +A   +Y++VP+ ++ ++ +K+  K   + +   D+ I
Sbjct: 414 S----FASFDWQAILFSYMSVPLFLILFIYYKIRHKTKLIPLEEVDLTI 458


Lambda     K      H
   0.324    0.139    0.431 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 765
Number of extensions: 33
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 588
Length of database: 470
Length adjustment: 35
Effective length of query: 553
Effective length of database: 435
Effective search space:   240555
Effective search space used:   240555
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.0 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.5 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources