Potential Gaps in catabolism of small carbon sources in Rhizobium freirei PRF 81
Found 19 low-confidence and 22 medium-confidence steps on the best paths for 62 pathways.
| Pathway | Step | Best candidate | 2nd candidate |
|---|
| 2-oxoglutarate | kgtP: 2-oxoglutarate:H+ symporter KgtP | RHSP_RS23870 | RHSP_RS10290 |
| 4-hydroxybenzoate | pcaK: 4-hydroxybenzoate transporter pcaK | | |
| citrate | SLC13A5: citrate:Na+ symporter | | |
| citrulline | AO353_03040: ABC transporter for L-Citrulline, ATPase component | RHSP_RS08840 | RHSP_RS00540 |
| citrulline | AO353_03045: ABC transporter for L-Citrulline, permease component 2 | RHSP_RS22960 | RHSP_RS24400 |
| citrulline | AO353_03050: ABC transporter for L-Citrulline, permease component 1 | RHSP_RS24395 | RHSP_RS22965 |
| D-serine | dsdA: D-serine ammonia-lyase | RHSP_RS12915 | RHSP_RS31295 |
| deoxyinosine | bmpA: deoxyinosine ABC transporter, substrate-binding component | RHSP_RS30400 | |
| deoxyinosine | nupB: deoxyinosine ABC transporter, permease component 1 | RHSP_RS30410 | |
| deoxyinosine | nupC': deoxyinosine ABC transporter, permease component 2 | RHSP_RS15685 | RHSP_RS30415 |
| deoxyribonate | deoxyribonate-dehyd: 2-deoxy-D-ribonate 3-dehydrogenase | RHSP_RS32970 | RHSP_RS12295 |
| deoxyribonate | deoxyribonate-transport: 2-deoxy-D-ribonate transporter | RHSP_RS25455 | |
| deoxyribonate | ketodeoxyribonate-cleavage: 2-deoxy-3-keto-D-ribonate cleavage enzyme | RHSP_RS06675 | |
| deoxyribose | deoP: deoxyribose transporter | | |
| galacturonate | exuT: D-galacturonate transporter ExuT | | |
| galacturonate | garD: meso-galactarate dehydratase (L-threo-forming) GarD | RHSP_RS13665 | RHSP_RS22975 |
| gluconate | gntT: gluconate:H+ symporter GntT | | |
| glucose-6-P | uhpT: glucose-6-phosphate:phosphate antiporter | | |
| glucuronate | exuT: D-glucuronate:H+ symporter ExuT | | |
| glucuronate | gci: D-glucaro-1,4-lactone cycloisomerase | RHSP_RS13965 | |
| glycerol | glpF: glycerol facilitator glpF | RHSP_RS10285 | RHSP_RS08020 |
| histidine | hutG': N-formylglutamate amidohydrolase | RHSP_RS13170 | |
| lactose | lacE: lactose ABC transporter, substrate-binding component | | |
| lactose | lacG: lactose ABC transporter, permease component 2 | RHSP_RS29495 | RHSP_RS17895 |
| lysine | hisP: L-lysine ABC transporter, ATPase component HisP | RHSP_RS08130 | RHSP_RS00540 |
| mannitol | mtlG: polyol ABC transporter, permease component 2 (MtlG/SmoG) | RHSP_RS13765 | RHSP_RS20465 |
| phenylacetate | paaT: phenylacetate transporter Paa | | |
| phenylalanine | iorAB: phenylpyruvate:ferredoxin oxidoreductase, fused IorA/IorB | RHSP_RS19440 | |
| phenylalanine | livH: L-phenylalanine ABC transporter, permease component 1 (LivH) | RHSP_RS00465 | RHSP_RS18045 |
| phenylalanine | livJ: L-phenylalanine ABC transporter, substrate-binding component LivJ/LivK | RHSP_RS00440 | RHSP_RS24770 |
| serine | braC: L-alanine/L-serine/L-threonine ABC transporter, substrate binding protein (BraC/NatB) | RHSP_RS00440 | RHSP_RS24770 |
| serine | braD: L-alanine/L-serine/L-threonine ABC transporter, permease component 1 (BraD/NatD) | RHSP_RS00465 | RHSP_RS09460 |
| sorbitol | sdh: sorbitol dehydrogenase | RHSP_RS24890 | RHSP_RS09190 |
| threonine | braC: L-alanine/L-serine/L-threonine ABC transporter, substrate binding protein (BraC/NatB) | RHSP_RS00440 | RHSP_RS24770 |
| threonine | braD: L-alanine/L-serine/L-threonine ABC transporter, permease component 1 (BraD/NatD) | RHSP_RS00465 | RHSP_RS09460 |
| thymidine | nupG: thymidine permease NupG/XapB | | |
| tryptophan | aroP: tryptophan:H+ symporter AroP | RHSP_RS26460 | |
| tryptophan | tnaA: tryptophanase | | |
| tyrosine | aroP: L-tyrosine transporter (AroP/FywP) | RHSP_RS26460 | |
| valine | bch: 3-hydroxyisobutyryl-CoA hydrolase | RHSP_RS30990 | RHSP_RS02715 |
| valine | mmsA: methylmalonate-semialdehyde dehydrogenase | RHSP_RS04195 | RHSP_RS19350 |
Confidence: high confidence medium confidence low confidence
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Links
Downloads
Related tools
About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory