GapMind for catabolism of small carbon sources

 

Protein WP_004109454.1 in Rhizobium freirei PRF 81

Annotation: NCBI__GCF_000359745.1:WP_004109454.1

Length: 384 amino acids

Source: GCF_000359745.1 in NCBI

Candidate for 18 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
D-maltose catabolism aglG hi ABC transporter for D-Maltose and D-Trehalose, permease component 2 (characterized) 65% 100% 499.6 Membrane bound sugar transport protein, component of The glucosylglycerol uptake transporter (functions in osmoprotection and also transports sucrose and trehalose (Mikkat and Hagemann, 2000) (most similar to 3.A.1.1.8) 62% 298.5
sucrose catabolism aglG hi ABC transporter for D-Maltose and D-Trehalose, permease component 2 (characterized) 65% 100% 499.6 Membrane bound sugar transport protein, component of The glucosylglycerol uptake transporter (functions in osmoprotection and also transports sucrose and trehalose (Mikkat and Hagemann, 2000) (most similar to 3.A.1.1.8) 62% 298.5
trehalose catabolism aglG hi ABC transporter for D-Maltose and D-Trehalose, permease component 2 (characterized) 65% 100% 499.6 Membrane bound sugar transport protein, component of The glucosylglycerol uptake transporter (functions in osmoprotection and also transports sucrose and trehalose (Mikkat and Hagemann, 2000) (most similar to 3.A.1.1.8) 62% 298.5
D-cellobiose catabolism aglG' hi Inner membrane ABC transporter permease protein (characterized, see rationale) 56% 96% 407.5 Membrane bound sugar transport protein, component of The glucosylglycerol uptake transporter (functions in osmoprotection and also transports sucrose and trehalose (Mikkat and Hagemann, 2000) (most similar to 3.A.1.1.8) 62% 298.5
D-glucose catabolism aglG' hi Inner membrane ABC transporter permease protein (characterized, see rationale) 56% 96% 407.5 Membrane bound sugar transport protein, component of The glucosylglycerol uptake transporter (functions in osmoprotection and also transports sucrose and trehalose (Mikkat and Hagemann, 2000) (most similar to 3.A.1.1.8) 62% 298.5
lactose catabolism aglG' hi Inner membrane ABC transporter permease protein (characterized, see rationale) 56% 96% 407.5 Membrane bound sugar transport protein, component of The glucosylglycerol uptake transporter (functions in osmoprotection and also transports sucrose and trehalose (Mikkat and Hagemann, 2000) (most similar to 3.A.1.1.8) 62% 298.5
D-maltose catabolism aglG' hi Inner membrane ABC transporter permease protein (characterized, see rationale) 56% 96% 407.5 Membrane bound sugar transport protein, component of The glucosylglycerol uptake transporter (functions in osmoprotection and also transports sucrose and trehalose (Mikkat and Hagemann, 2000) (most similar to 3.A.1.1.8) 62% 298.5
sucrose catabolism aglG' hi Inner membrane ABC transporter permease protein (characterized, see rationale) 56% 96% 407.5 Membrane bound sugar transport protein, component of The glucosylglycerol uptake transporter (functions in osmoprotection and also transports sucrose and trehalose (Mikkat and Hagemann, 2000) (most similar to 3.A.1.1.8) 62% 298.5
trehalose catabolism aglG' hi Inner membrane ABC transporter permease protein (characterized, see rationale) 56% 96% 407.5 Membrane bound sugar transport protein, component of The glucosylglycerol uptake transporter (functions in osmoprotection and also transports sucrose and trehalose (Mikkat and Hagemann, 2000) (most similar to 3.A.1.1.8) 62% 298.5
L-arabinose catabolism xacI lo Xylose/arabinose import permease protein XacI (characterized, see rationale) 31% 79% 118.2 ABC transporter for D-Maltose and D-Trehalose, permease component 2 65% 499.6
D-cellobiose catabolism glcU lo GlcU, component of Glucose, mannose, galactose porter (characterized) 32% 83% 117.9 ABC transporter for D-Maltose and D-Trehalose, permease component 2 65% 499.6
D-galactose catabolism glcU lo GlcU, component of Glucose, mannose, galactose porter (characterized) 32% 83% 117.9 ABC transporter for D-Maltose and D-Trehalose, permease component 2 65% 499.6
D-glucose catabolism glcU lo GlcU, component of Glucose, mannose, galactose porter (characterized) 32% 83% 117.9 ABC transporter for D-Maltose and D-Trehalose, permease component 2 65% 499.6
lactose catabolism glcU lo GlcU, component of Glucose, mannose, galactose porter (characterized) 32% 83% 117.9 ABC transporter for D-Maltose and D-Trehalose, permease component 2 65% 499.6
D-maltose catabolism glcU lo GlcU, component of Glucose, mannose, galactose porter (characterized) 32% 83% 117.9 ABC transporter for D-Maltose and D-Trehalose, permease component 2 65% 499.6
D-mannose catabolism glcU lo GlcU, component of Glucose, mannose, galactose porter (characterized) 32% 83% 117.9 ABC transporter for D-Maltose and D-Trehalose, permease component 2 65% 499.6
sucrose catabolism glcU lo GlcU, component of Glucose, mannose, galactose porter (characterized) 32% 83% 117.9 ABC transporter for D-Maltose and D-Trehalose, permease component 2 65% 499.6
trehalose catabolism glcU lo GlcU, component of Glucose, mannose, galactose porter (characterized) 32% 83% 117.9 ABC transporter for D-Maltose and D-Trehalose, permease component 2 65% 499.6

Sequence Analysis Tools

View WP_004109454.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MLGNLGRIGPARLFVHGAVLLIVLLWLLPTLGIFVTALRGKDQIVVSGWWTAFAGSSRTT
AARLDGPDKAKPDGANFVISGNIAVDGGGSIQSFGQRVQEPSAYKVGAPAELENGETLTI
DANGSYRYQKNGPFGADARAKRVYLTVATPPQFTMDNYRTVLAGEGIGQSFINSLTVTIP
ATVIPILIAAFAAYALAWMEFPGRALLIALVVGLIVVPLQMSLIPLLRIYNEIGQIFGVP
SKTYPGIWMAHTAFGLPLAIYLLRNYIAGLPKEIIESARVDGASDFDIFVKIILPLSFPA
LASFAIFQFLWVWNDLLIAMVFLGTDKDHLVLTAALNALLGSRGGNWEILTASAFVTIII
PLLVFFGLQRYLVRGLLAGSVKGG

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory