Protein WP_004127637.1 in Rhizobium freirei PRF 81
Annotation: NCBI__GCF_000359745.1:WP_004127637.1
Length: 354 amino acids
Source: GCF_000359745.1 in NCBI
Candidate for 11 steps in catabolism of small carbon sources
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|
| D-glucosamine (chitosamine) catabolism | SM_b21216 | hi | ABC transporter for D-Glucosamine, ATPase component (characterized) | 75% | 99% | 505.8 | ABC transporter for D-Glucose-6-Phosphate, ATPase component | 53% | 368.6 |
| D-cellobiose catabolism | gtsD | med | ABC transporter for D-Glucose-6-Phosphate, ATPase component (characterized) | 53% | 94% | 368.6 | ABC transporter for D-Glucosamine, ATPase component | 75% | 505.8 |
| D-glucose catabolism | gtsD | med | ABC transporter for D-Glucose-6-Phosphate, ATPase component (characterized) | 53% | 94% | 368.6 | ABC transporter for D-Glucosamine, ATPase component | 75% | 505.8 |
| lactose catabolism | gtsD | med | ABC transporter for D-Glucose-6-Phosphate, ATPase component (characterized) | 53% | 94% | 368.6 | ABC transporter for D-Glucosamine, ATPase component | 75% | 505.8 |
| D-maltose catabolism | gtsD | med | ABC transporter for D-Glucose-6-Phosphate, ATPase component (characterized) | 53% | 94% | 368.6 | ABC transporter for D-Glucosamine, ATPase component | 75% | 505.8 |
| sucrose catabolism | gtsD | med | ABC transporter for D-Glucose-6-Phosphate, ATPase component (characterized) | 53% | 94% | 368.6 | ABC transporter for D-Glucosamine, ATPase component | 75% | 505.8 |
| trehalose catabolism | gtsD | med | ABC transporter for D-Glucose-6-Phosphate, ATPase component (characterized) | 53% | 94% | 368.6 | ABC transporter for D-Glucosamine, ATPase component | 75% | 505.8 |
| D-xylose catabolism | gtsD | med | ABC transporter for D-Glucose-6-Phosphate, ATPase component (characterized) | 53% | 94% | 368.6 | ABC transporter for D-Glucosamine, ATPase component | 75% | 505.8 |
| D-galactose catabolism | PfGW456L13_1897 | med | ABC transporter for D-Galactose and D-Glucose, ATPase component (characterized) | 52% | 94% | 361.7 | ABC transporter for D-Glucosamine, ATPase component | 75% | 505.8 |
| D-cellobiose catabolism | SMc04256 | med | ABC transporter for D-Cellobiose and D-Salicin, ATPase component (characterized) | 48% | 100% | 330.9 | ABC transporter for D-Glucosamine, ATPase component | 75% | 505.8 |
| glycerol catabolism | glpS | lo | ABC transporter for Glycerol, ATPase component 1 (characterized) | 37% | 96% | 204.9 | ABC transporter for D-Glucosamine, ATPase component | 75% | 505.8 |
Sequence Analysis Tools
View WP_004127637.1 at NCBI
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
Fitness BLAST: loading...
Sequence
MSALEIQNIRKSYGALETLKGIDISLESGEFLVLLGSSGCGKSTLLNIIAGLAEATSGDI
RIGDRSVLGVHPKDRDIAMVFQSYALYPNLSVHRNIGFGLEMRKVPAAERDKAVREAAKL
LQIEALLERKPSQLSGGQRQRVAIGRALVRKPEVFLFDEPLSNLDAKLRMEMRTELKRLH
QMLKTTVVYVTHDQIEAMTLATRIAVMRDGRIEQLGRPDEIYNHPATLYVATFVGAPPMN
LLNATADQGSLRIDGTSITLPMPTAKAEIKQGQELVVGIRPETLYLDEQGQLEAVCEVAE
LTGPELIVTAHTGSQRLMACLPPRTTIAEGQTLKLGFDASALHLFDRATGRRCQ
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
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About GapMind
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using
ublast (a fast alternative to protein BLAST)
against a database of manually-curated proteins (most of which are experimentally characterized) or by using
HMMer with enzyme models (usually from
TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
- ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
- HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.
where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").
Otherwise, a candidate is "medium confidence" if either:
- ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
- ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
- HMMer finds a hit (regardless of coverage or other bits).
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps."
For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways.
For diverse bacteria and archaea that can utilize a carbon source, there is a complete
high-confidence catabolic pathway (including a transporter) just 38% of the time, and
there is a complete medium-confidence pathway 63% of the time.
Gaps may be due to:
- our ignorance of proteins' functions,
- omissions in the gene models,
- frame-shift errors in the genome sequence, or
- the organism lacks the pathway.
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory