GapMind for catabolism of small carbon sources

 

Protein WP_037155072.1 in Rhizobium freirei PRF 81

Annotation: NCBI__GCF_000359745.1:WP_037155072.1

Length: 309 amino acids

Source: GCF_000359745.1 in NCBI

Candidate for 11 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
myo-inositol catabolism PS417_11885 hi Rhizopine-binding protein (characterized, see rationale) 49% 100% 280 D-galactose-binding periplasmic protein DGAL aka MglB aka B2150, component of Galactose/glucose (methyl galactoside) porter 36% 168.3
D-cellobiose catabolism mglB lo D-galactose-binding periplasmic protein DGAL aka MglB aka B2150, component of Galactose/glucose (methyl galactoside) porter (characterized) 36% 99% 168.3 Inositol transport system sugar-binding protein 52% 276.6
D-galactose catabolism mglB lo D-galactose-binding periplasmic protein DGAL aka MglB aka B2150, component of Galactose/glucose (methyl galactoside) porter (characterized) 36% 99% 168.3 Inositol transport system sugar-binding protein 52% 276.6
D-glucose catabolism mglB lo D-galactose-binding periplasmic protein DGAL aka MglB aka B2150, component of Galactose/glucose (methyl galactoside) porter (characterized) 36% 99% 168.3 Inositol transport system sugar-binding protein 52% 276.6
lactose catabolism mglB lo D-galactose-binding periplasmic protein DGAL aka MglB aka B2150, component of Galactose/glucose (methyl galactoside) porter (characterized) 36% 99% 168.3 Inositol transport system sugar-binding protein 52% 276.6
D-maltose catabolism mglB lo D-galactose-binding periplasmic protein DGAL aka MglB aka B2150, component of Galactose/glucose (methyl galactoside) porter (characterized) 36% 99% 168.3 Inositol transport system sugar-binding protein 52% 276.6
sucrose catabolism mglB lo D-galactose-binding periplasmic protein DGAL aka MglB aka B2150, component of Galactose/glucose (methyl galactoside) porter (characterized) 36% 99% 168.3 Inositol transport system sugar-binding protein 52% 276.6
trehalose catabolism mglB lo D-galactose-binding periplasmic protein DGAL aka MglB aka B2150, component of Galactose/glucose (methyl galactoside) porter (characterized) 36% 99% 168.3 Inositol transport system sugar-binding protein 52% 276.6
xylitol catabolism PS417_12055 lo Putative xylitol transport system substrate-binding protein; SubName: Full=Sugar ABC transporter substrate-binding protein (characterized, see rationale) 30% 86% 133.3 Inositol transport system sugar-binding protein 52% 276.6
myo-inositol catabolism ibpA lo Inositol ABC transporter, periplasmic inositol-binding protein IbpA, component of The myoinositol (high affinity)/ D-ribose (low affinity) transporter IatP/IatA/IbpA. The structure of IbpA with myoinositol bound has been solved (characterized) 30% 95% 119 Inositol transport system sugar-binding protein 52% 276.6
L-arabinose catabolism araUsh lo Periplasmic binding protein/LacI transcriptional regulator (characterized, see rationale) 32% 92% 116.3 Inositol transport system sugar-binding protein 52% 276.6

Sequence Analysis Tools

View WP_037155072.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MKKFILGSAMALLLSTAAHAQTIGVSMALFDDNFLTVLRNGMQDYAKGMSGVTLQVEDAQ
NDVAKQQSQIQNFIASKVDAIIVNPVDTDATAAISKLAADAKIPLVYVNREPANVDSLPD
KQAFVASNEQESGTLETKEVCRILGGKGKAVVMMGELSNQAARMRTKDVHDVLATDGCKG
IKIVQEQTANWQRTQGADLMTNWLSSGLEFDAVISNNDEMAIGAIQALKAAGKPMDKIVV
AGVDATQDALAAMQAGDLKVTVFQDAAGQGKGAVDAALKLAKGDKIDKKVYIPFQLVTPT
NVKDFVKKN

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory