Alignments for a candidate for dddA in Rhizobium freirei PRF 81

GapMind for catabolism of small carbon sources

Alignments for a candidate for dddA in Rhizobium freirei PRF 81

Align 3-hydroxypropionate dehydrogenase (EC 1.1.1.59) (characterized)
to candidate WP_004126341.1 RHSP_RS27110 glucose-methanol-choline oxidoreductase

Query= metacyc::MONOMER-15202
         (579 letters)



>NCBI__GCF_000359745.1:WP_004126341.1
          Length = 551

 Score =  326 bits (836), Expect = 1e-93
 Identities = 208/544 (38%), Positives = 291/544 (53%), Gaps = 24/544 (4%)

Query: 35  AFDYIVVGAGTAGCLLANRLSADPANRVLLIEAGGRDNYHWIHIPVGYLYCINNPRTDWR 94
           AFDYI+ GAG AGC+LANRLS DP  +V L+EAGG D     H+P G+        + W 
Sbjct: 2   AFDYIITGAGPAGCVLANRLSEDPNVKVFLLEAGGGDWNPLFHMPAGFAKMTKGVAS-WG 60

Query: 95  FRTEPDPGLNGRSLIYPRGKTLGGCSSINGMLYLRGQARDYDGWAELTGDDAWRWDNCLP 154
           + T P   + GR L Y + K +GG SSIN  LY RG + DYD WA   G + W + + LP
Sbjct: 61  WHTVPQKHMKGRVLRYTQAKVIGGGSSINAQLYTRGNSADYDLWASEDGCEGWDYRSILP 120

Query: 155 DFMRHEDHYRLDEGGDADPDHYKFHGHGGEWRIEKQRLKWQVLADFATAAVEAGVPRTRD 214
            F R ED+ R  +          +H +GG   +        +   +  A  E G+P   D
Sbjct: 121 YFKRAEDNQRFADD---------YHSYGGPLGVSMPAAPLPICDAYIRAGQELGIPYNHD 171

Query: 215 FNRGDNEGVDAFEVNQRSGWRWNASKAFLRGVEQRGNLTVWHSTQVLKLDFASGEGSEPR 274
           FN     GV  +++ QR   R +AS A+L  ++ R NLTV    +V ++     EG    
Sbjct: 172 FNGRQQAGVGFYQLTQRDRRRSSASMAYLSPIKDRKNLTVRTGARVSRIVL---EGKR-- 226

Query: 275 CCGVTVERAGKKVVTTARC--EVVLSAGAIGSPQLLQLSGIGPTALLAEHAIPVVADLPG 332
              V VE  GK  V   R   EV++++GAIGSP+LLQ SGIGP   L    + V  DLPG
Sbjct: 227 --AVGVEIVGKNGVEIVRADREVLVTSGAIGSPKLLQQSGIGPGDHLRSVGVNVQHDLPG 284

Query: 333 VGENLQDHLQIRSIYKVKGAKTLNTMANSLIGKAKIGLEYILKRSGPMSMAPSQLCIFTR 392
           VG N+QDHL +  I +  G  T + +A  L      G++Y+L ++GP++ +  +   F  
Sbjct: 285 VGGNMQDHLDLFVIAECTGDHTYDGVAK-LHRTLWAGIQYVLFKTGPVASSLFETGGFWY 343

Query: 393 SSKEYEHPNLEYHVQPLS-LEAFGQPLHDFPAITASVCNLNPTSRGTVRIKSGNPRQAPA 451
           +      P++++H+   S +EA  + L +   +T +   L+P SRGTVR+ S +P  AP 
Sbjct: 344 ADPNARSPDIQFHLGLGSGIEAGVERLKN-AGVTLNSAYLHPRSRGTVRLSSADPAAAPL 402

Query: 452 ISPNYLSTEEDRQVAADSLRVTRHIASQPAFAKYDPEEFKPGVQYQSDEDLARLAGDIGT 511
           I PNY S   DR+++ + L+V R I  Q A   +   E  PG +  +DE L         
Sbjct: 403 IDPNYWSDPHDREMSLEGLKVAREIMQQAALKPFVLAERLPGPKVMTDEQLFEYGCANAK 462

Query: 512 TIFHPVGTAKMGRDDDPMAVVDSHLRVRGVTGLRVVDASIMPTITSGNTNSPTLMIAEKA 571
           T  HPVGT KMG    P AVV   L+V G+ GLRV D+S+MP + S NTN+PT+M+ EK 
Sbjct: 463 TDHHPVGTCKMG--TGPEAVVGLDLKVHGLEGLRVCDSSVMPRVPSCNTNAPTIMVGEKG 520

Query: 572 AGWI 575
           A  I
Sbjct: 521 ADLI 524


Lambda     K      H
   0.318    0.135    0.418 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 906
Number of extensions: 51
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 579
Length of database: 551
Length adjustment: 36
Effective length of query: 543
Effective length of database: 515
Effective search space:   279645
Effective search space used:   279645
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources