Alignments for a candidate for MFS-glucose in Rhizobium freirei PRF 81

GapMind for catabolism of small carbon sources

Alignments for a candidate for MFS-glucose in Rhizobium freirei PRF 81

Align Glucose/galactose porter (characterized)
to candidate WP_004116000.1 RHSP_RS10145 sugar MFS transporter

Query= TCDB::P0C105
         (412 letters)



>NCBI__GCF_000359745.1:WP_004116000.1
          Length = 427

 Score =  618 bits (1593), Expect = 0.0
 Identities = 319/421 (75%), Positives = 352/421 (83%), Gaps = 11/421 (2%)

Query: 2   ATSIPTNNPLHTETSSQKNYGFALTSLTLLFFMWGFITCLNDILIPHLKNVFQLNYTQSM 61
           ATS    +   +    Q +Y FAL +LT LFFMWGFITCLNDIL+PHLK+VFQLNYTQSM
Sbjct: 5   ATSSAPTSVRASAHQGQGSYQFALIALTSLFFMWGFITCLNDILVPHLKSVFQLNYTQSM 64

Query: 62  LIQFCFFGAYFIVSLPAGQLVKRISYKRGIVVGLIVAAIGCALFIPAASYRVYALFLGAL 121
           LIQ CFFGAYFIVSLPAG LVKRI+YK GIVVGL +AA+GCALFIPAASYRVYALFLGAL
Sbjct: 65  LIQLCFFGAYFIVSLPAGALVKRITYKWGIVVGLSIAAVGCALFIPAASYRVYALFLGAL 124

Query: 122 FVLASGVTILQVAANPYVTILGKPETAASRLTLTQAFNSLGTTVAPVFGAVLILSAATDA 181
           FVLA+G+TILQVAANPYVT+LG P+TAASRL LTQAFNSLGTT+AP FGA+LILSAAT A
Sbjct: 125 FVLATGITILQVAANPYVTVLGPPDTAASRLNLTQAFNSLGTTIAPYFGAMLILSAATVA 184

Query: 182 TVNA-----------EADAVRFPYLLLALAFTVLAIIFAILKPPDVQEDEPALSDKKEGS 230
             NA           EA AV+ PYL LA AF +LA IFA LK P+V+++E     +  GS
Sbjct: 185 AANATPEQIDAMRLAEAGAVKLPYLFLAAAFLLLAAIFAALKLPEVEDEEAVEPMRGGGS 244

Query: 231 AWQYRHLVLGAIGIFVYVGAEVSVGSFLVNFLSDPTVAGLSETDAAHHVAYFWGGAMVGR 290
           AWQYRHLVLGAIGIF+YVGAEVS+GSFLVNFLS P +A LSE DAA +V+YFWGGAM+GR
Sbjct: 245 AWQYRHLVLGAIGIFLYVGAEVSIGSFLVNFLSQPDIAHLSEADAARYVSYFWGGAMLGR 304

Query: 291 FIGSAAMRYIDDGKALAFNAFVAIILLFITVATTGHIAMWSVLAIGLFNSIMFPTIFSLA 350
           F GS  MR IDDGKALAFNA VAI+LL ITV TTGH+AMWSVLAIGLFNSIMFPTIFSLA
Sbjct: 305 FAGSIIMRQIDDGKALAFNAAVAIVLLVITVLTTGHVAMWSVLAIGLFNSIMFPTIFSLA 364

Query: 351 LHGLGSHTSQGSGILCLAIVGGAIVPLIQGALADAIGIHLAFLMPIICYAYIAFYGLIGS 410
           LHGLG +TSQGSGILCLAIVGGAI+P+IQG LAD IGIHLAFLMPIICY YIAFYGL G 
Sbjct: 365 LHGLGKYTSQGSGILCLAIVGGAILPVIQGGLADTIGIHLAFLMPIICYVYIAFYGLKGH 424

Query: 411 K 411
           K
Sbjct: 425 K 425


Lambda     K      H
   0.328    0.141    0.420 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 742
Number of extensions: 28
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 412
Length of database: 427
Length adjustment: 32
Effective length of query: 380
Effective length of database: 395
Effective search space:   150100
Effective search space used:   150100
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.7 bits)
S2: 50 (23.9 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources