Alignments for a candidate for rhaT' in Rhizobium freirei PRF 81

GapMind for catabolism of small carbon sources

Alignments for a candidate for rhaT' in Rhizobium freirei PRF 81

Align RhaT, component of Rhamnose porter (Richardson et al., 2004) (Transport activity is dependent on rhamnokinase (RhaK; AAQ92412) activity (Richardson and Oresnik, 2007) This could be an example of group translocation!) (characterized)
to candidate WP_004124909.1 RHSP_RS24795 sugar ABC transporter ATP-binding protein

Query= TCDB::Q7BSH4
         (512 letters)



>NCBI__GCF_000359745.1:WP_004124909.1
          Length = 511

 Score =  825 bits (2130), Expect = 0.0
 Identities = 429/512 (83%), Positives = 461/512 (90%), Gaps = 1/512 (0%)

Query: 1   MNAAFQQPVTDSKTGDAPAILEMRGISQIFPGVKALDNVSIALHPGTVTALIGENGAGKS 60
           M    Q+ + D+   ++ AILEMRGISQIFPGVKALD VSI+L+PG VTALIGENGAGKS
Sbjct: 1   MTTVLQRSLADTPAANSQAILEMRGISQIFPGVKALDGVSISLYPGKVTALIGENGAGKS 60

Query: 61  TLVKILTGIYRPNEGEILVDGRPTTFASAQAAIDAGVTAIHQETVLFDELTVAENIFLGH 120
           TLVKILTGIYRPNEGEILVDG+P  FASAQAAIDAGVTAIHQETVLFDELTVAENIFLGH
Sbjct: 61  TLVKILTGIYRPNEGEILVDGKPMAFASAQAAIDAGVTAIHQETVLFDELTVAENIFLGH 120

Query: 121 APRTRFRTIDWQTMNSRSKALLTALESNIDPTIRLKDLSIAQRHLVAIARALSIEARIVI 180
           APRT +RTIDW+ MN RSK LL +LES IDPTIRLKDLSIAQRHLVAIARALSIEARIVI
Sbjct: 121 APRTSWRTIDWKRMNGRSKELLQSLESAIDPTIRLKDLSIAQRHLVAIARALSIEARIVI 180

Query: 181 MDEPTAALSRKEIDDLFRIVRGLKEQGKAILFISHKFDELYEIADDFVVFPRRSRRPVRG 240
           MDEPTAALSRKEIDDLFRIV GLK +GKAILFISHKFDELYEIAD+FVVF R  R    G
Sbjct: 181 MDEPTAALSRKEIDDLFRIVEGLKARGKAILFISHKFDELYEIADNFVVF-RDGRDVGHG 239

Query: 241 VSRKTPQDEIVRMMVGRDVENVFPKIDVAIGGPVLEIRNYSHRTEFRDISFTLRKGEILG 300
             + TPQDEIVRMMVGRDV++ FPK+ V IG  VLE+  Y HRTEFRDISF LR+GEILG
Sbjct: 240 DLKMTPQDEIVRMMVGRDVKDAFPKVPVTIGDTVLEVEKYCHRTEFRDISFKLRRGEILG 299

Query: 301 VYGLIGAGRSELSQSLFGITKPLSGKMVLEGQEITIHSPQDAIRAGIVYVPEERGRHGLA 360
           VYGLIGAGRSEL QSLFGITKPLSG++ L+GQEI + SPQDAI AGIVYVPEERGRHGLA
Sbjct: 300 VYGLIGAGRSELCQSLFGITKPLSGRLTLDGQEIQVRSPQDAIGAGIVYVPEERGRHGLA 359

Query: 361 LPMPIFQNMTLPSLARTSRRGFLRAANEFALARKYAERLDLRAAALSVPVGTLSGGNQQK 420
           LPMPI+QNM+LPSL RTSR+GFL+AANEFALARKYAERLDLRAAALSVPVGTLSGGNQQK
Sbjct: 360 LPMPIYQNMSLPSLGRTSRKGFLKAANEFALARKYAERLDLRAAALSVPVGTLSGGNQQK 419

Query: 421 VVIGKWLATAPKVIILDEPTKGIDIGSKAAVHGFISELAAEGLSIIMVSSELPEIIGMSD 480
           VVIGKWLAT PK+IILDEPTKGIDIGSKAAVHGFISELAAEGLSIIM+SSELPEI+GMSD
Sbjct: 420 VVIGKWLATQPKIIILDEPTKGIDIGSKAAVHGFISELAAEGLSIIMISSELPEILGMSD 479

Query: 481 RVLVMKEGLSAGIFERAELSPEALVRAATGNA 512
           RVLVMKEGLSAG+FER  L+PE LVRAATGNA
Sbjct: 480 RVLVMKEGLSAGLFEREGLTPETLVRAATGNA 511


Lambda     K      H
   0.320    0.137    0.382 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 893
Number of extensions: 23
Number of successful extensions: 6
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 512
Length of database: 511
Length adjustment: 35
Effective length of query: 477
Effective length of database: 476
Effective search space:   227052
Effective search space used:   227052
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources