Alignments for a candidate for glcD in Rhizobium freirei PRF 81

GapMind for catabolism of small carbon sources

Alignments for a candidate for glcD in Rhizobium freirei PRF 81

Align glycolate oxidase subunit glcD (characterized)
to candidate WP_004109955.1 RHSP_RS04905 FAD-binding oxidoreductase

Query= CharProtDB::CH_024646
         (499 letters)



>NCBI__GCF_000359745.1:WP_004109955.1
          Length = 476

 Score =  164 bits (416), Expect = 5e-45
 Identities = 137/451 (30%), Positives = 204/451 (45%), Gaps = 27/451 (5%)

Query: 40  EIIPYECDGLSAYRTRPLLVVLPKQMEQVTAILAVCHRLRVPVVTRGAGTGLSGGALPLE 99
           EI P+  +    Y     +++ P  +E+V++IL +       +V +   TGL GG  P E
Sbjct: 29  EIAPHLVENRGLYHGASPMLLKPGSVEEVSSILKLASETGAAIVPQTGNTGLVGGQTPRE 88

Query: 100 KG--VLLVMARFKEILDINPVGRRARVQPGVRNLAISQAVAPHNLYYAPDPSSQIACSIG 157
            G  ++L + R   + D++PVG    V  G     + +A A H   +     S+ +C I 
Sbjct: 89  GGSDIILSLERMNRVRDVDPVGNTIVVDGGCILADVHKAAADHGRMFPLSLGSEGSCRIA 148

Query: 158 GNVAENAGGVHCLKYGLTVHNLLKIEVQTLDGEALT-LGSDALDSPGFDLLALFTGSEGM 216
           GN++ NAGG   L YG      L +EV    GE    L     D+ G+DL  LF G+EG 
Sbjct: 149 GNLSTNAGGTAVLAYGNMRQLCLGLEVVLPTGEIWNGLRRLKKDNTGYDLRDLFVGAEGT 208

Query: 217 LGVTTEVTVKLLPKPPVARVLLASFDSVEKA-GLAVGDIIANGIIPGGLEMMDNLSIR-A 274
           LGV T   +KL P+P   +V  A   SV+ A  L        G    G E+M  + +   
Sbjct: 209 LGVITGAVLKLFPQPLGHQVAFAGLQSVDDALTLFKNASSLCGTALTGFELMPRIGVEFT 268

Query: 275 AEDFIHAGYPVDAEAILLCELDGVESDVQEDCERVNDILLKAG-----ATDVRLAQDEAE 329
           A        P++        +D   SD  E  ER+   LL+ G       D  +A  EA+
Sbjct: 269 ARHIPGVRDPLETAHPWYVLIDISTSDSAETAERMMAALLEQGYEAGLIQDATIASSEAQ 328

Query: 330 RVRFWAGRKNAF----PAVGRISPDYYCMDGTIPRRALPGVLEGIARLSQQYDLRVANVF 385
           R   W  R++      P  G I  D       IP+         +A +      RV    
Sbjct: 329 RQAIWHMRESMSDAQKPEGGSIKHDVSVPVAQIPQFMAEAEKAVVAAMP---GARVCAFG 385

Query: 386 HAGDGNMHPLILFDANEPGEFARAEELG-----GKILE-LCVEVGGSISGEHGIGREKIN 439
           H GDGN+H    ++ ++P    +AE +G      KI+  L ++ GGSIS EHGIG+ K +
Sbjct: 386 HMGDGNIH----YNISQPVGADKAEFIGRWREMNKIVHGLVLQHGGSISAEHGIGQLKRD 441

Query: 440 QMCAQFNSDEITTFHAVKAAFDPDGLLNPGK 470
           ++ +  +  EI     +K AFDP G++NPGK
Sbjct: 442 ELASIRSDIEIDLMRRIKTAFDPAGIMNPGK 472


Lambda     K      H
   0.320    0.140    0.415 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 592
Number of extensions: 24
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 499
Length of database: 476
Length adjustment: 34
Effective length of query: 465
Effective length of database: 442
Effective search space:   205530
Effective search space used:   205530
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources