Align alcohol dehydrogenase (EC 1.1.1.1) (characterized)
to candidate WP_019613839.1 G327_RS0105925 L-threonine dehydrogenase
Query= BRENDA::P0DJA2 (383 letters) >NCBI__GCF_000381745.1:WP_019613839.1 Length = 382 Score = 490 bits (1261), Expect = e-143 Identities = 238/380 (62%), Positives = 294/380 (77%) Query: 3 SSTFYIPFVNEMGEGSLEKAIKDLNGSGFKNALIVSDAFMNKSGVVKQVADLLKAQGINS 62 SS F++P VN MG G L A+ + GF LIV+D ++ G+VKQV+DLL A + + Sbjct: 2 SSAFFMPTVNLMGAGCLSDAVASIKSQGFTQGLIVTDKILSSIGMVKQVSDLLAASEVKT 61 Query: 63 AVYDGVMPNPTVTAVLEGLKILKDNNSDFVISLGGGSPHDCAKAIALVATNGGEVKDYEG 122 V+DG PNPT+ V GL+ILKDN DF+ISLGGGSPHDCAK IALVA NGGE+ DYEG Sbjct: 62 VVFDGTQPNPTIGNVEAGLQILKDNQCDFIISLGGGSPHDCAKGIALVAANGGEIADYEG 121 Query: 123 IDKSKKPALPLMSINTTAGTASEMTRFCIITDEVRHVKMAIVDRHVTPMVSVNDPLLMVG 182 +D+S KP LPL+SINTTAGTASEMTRFCIITDE RH+KMAIVD++VTP++SVNDP LM+ Sbjct: 122 VDQSPKPQLPLISINTTAGTASEMTRFCIITDEARHIKMAIVDKNVTPLMSVNDPELMLA 181 Query: 183 MPKGLTAATGMDALTHAFEAYSSTAATPITDACALKAASMIAKNLKTACDNGKDMPAREA 242 P LTAATGMDALTHA EAY S AATPITDA A+KA +I ++L+TA +G+ + ARE Sbjct: 182 KPASLTAATGMDALTHAVEAYVSIAATPITDAVAIKAIEIIQQSLRTAVKDGQSIEAREQ 241 Query: 243 MAYAQFLAGMAFNNASLGYVHAMAHQLGGYYNLPHGVCNAVLLPHVLAYNASVVAGRLKD 302 MAYAQF+AGMAFNNASLGYVHAMAHQLGG+Y+LPHGVCNAVLLPHV +NA V RLKD Sbjct: 242 MAYAQFMAGMAFNNASLGYVHAMAHQLGGFYDLPHGVCNAVLLPHVQRFNAKVAPARLKD 301 Query: 303 VGVAMGLDIANLGDKEGAEATIQAVRDLAASIGIPANLTELGAKKEDVPLLADHALKDAC 362 V AMG+++ N+ ++EGA+A + A+ L+ +GIPA LT+LG K ED +L+++ALKDAC Sbjct: 302 VAKAMGVNVENMSNEEGADAALAAIAQLSTDVGIPAGLTDLGVKAEDFDVLSENALKDAC 361 Query: 363 ALTNPRQGDQKEVEELFLSA 382 TNP Q E+ E++ A Sbjct: 362 GFTNPIQATHAEITEIYRQA 381 Lambda K H 0.316 0.132 0.373 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 507 Number of extensions: 14 Number of successful extensions: 1 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 383 Length of database: 382 Length adjustment: 30 Effective length of query: 353 Effective length of database: 352 Effective search space: 124256 Effective search space used: 124256 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.6 bits) S2: 50 (23.9 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory