GapMind for catabolism of small carbon sources

 

Protein WP_038806526.1 in Streptococcus oralis 7747

Annotation: NCBI__GCF_000382825.1:WP_038806526.1

Length: 158 amino acids

Source: GCF_000382825.1 in NCBI

Candidate for 3 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
D-fructose catabolism levE lo PTS system fructose-specific EIIB component; EIIB-Fru; Fructose-specific phosphotransferase enzyme IIB component; lev-PTS; p18; EC 2.7.1.202 (characterized) 35% 97% 113.2 Phosphotransferase system sugar-specific EIIB component, component of Uptake porter/group translocator of galacto N-biose (Gal-β-1,3-GalNAc: galactose linked β-1,3 to N-acetylgalactosamine), lacto N-biose (Gal-β-1,3-GlcNAc: galactose linked β-1,3 to N-acetylglucosamine), and D-N-acetylgalactosamine (Bidart et al. 2014). The system is designated the GnbABCD system where GnbA = IIA, GnbB = IIB, GnbC = IIC, and GnbD = IID of the mannose-type PTS Enzyme II complex 64% 203.8
sucrose catabolism levE lo PTS system fructose-specific EIIB component; EIIB-Fru; Fructose-specific phosphotransferase enzyme IIB component; lev-PTS; p18; EC 2.7.1.202 (characterized) 35% 97% 113.2 Phosphotransferase system sugar-specific EIIB component, component of Uptake porter/group translocator of galacto N-biose (Gal-β-1,3-GalNAc: galactose linked β-1,3 to N-acetylgalactosamine), lacto N-biose (Gal-β-1,3-GlcNAc: galactose linked β-1,3 to N-acetylglucosamine), and D-N-acetylgalactosamine (Bidart et al. 2014). The system is designated the GnbABCD system where GnbA = IIA, GnbB = IIB, GnbC = IIC, and GnbD = IID of the mannose-type PTS Enzyme II complex 64% 203.8
D-mannose catabolism manX lo Mannose-specificPTS system, IIAB component, component of Mannose enzyme II complex, IIAB, IIC, IID. IIC/IID serve allows entry of some bacteriocins including pediocin (class IIa) and lactococcin A (class IIc) (Kjos et al., 2011). Transports and phosphorylates Glucose, Mannose and Glucosamine (characterized) 33% 50% 104 Phosphotransferase system sugar-specific EIIB component, component of Uptake porter/group translocator of galacto N-biose (Gal-β-1,3-GalNAc: galactose linked β-1,3 to N-acetylgalactosamine), lacto N-biose (Gal-β-1,3-GlcNAc: galactose linked β-1,3 to N-acetylglucosamine), and D-N-acetylgalactosamine (Bidart et al. 2014). The system is designated the GnbABCD system where GnbA = IIA, GnbB = IIB, GnbC = IIC, and GnbD = IID of the mannose-type PTS Enzyme II complex 64% 203.8

Sequence Analysis Tools

View WP_038806526.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MTIVGCRIDGRLIHGQVANLWAGKLNVSRIMVVDDEVVNNDIEKSGLKLATPPGVKLSIL
PVEKAAANILAGKYDSQRLFIVARKPDRFLGLVEAGVPLETLNVGNMSQTPETRAITRSI
NVVDKDVEDFHKLAEKGVKLTAQMVPNDPVSDFLSLLK

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory