GapMind for catabolism of small carbon sources

 

Alignments for a candidate for crr in Streptococcus oralis 7747

Align glucose-specific phosphotransferase enzyme IIA component; EC 2.7.1.- (characterized)
to candidate WP_038806370.1 HK29_RS07615 PTS beta-glucoside transporter subunit IIBCA

Query= CharProtDB::CH_088352
         (169 letters)



>NCBI__GCF_000382825.1:WP_038806370.1
          Length = 628

 Score =  100 bits (250), Expect = 4e-26
 Identities = 51/147 (34%), Positives = 85/147 (57%), Gaps = 2/147 (1%)

Query: 23  IIAPLSGEIVNIEDVPDVVFAEKIVGDGIAIKPTGNKMVAPVDGTIGKIFETNHAFSIES 82
           I  P+ G++V +E+V D VF+   +G GIA+KP+   + AP D  +   F T HA+ +++
Sbjct: 482 IQTPIVGDVVALENVDDPVFSSGAMGQGIAVKPSQGVVYAPADAEVSIAFATGHAYGLKT 541

Query: 83  DSGVELFVHFGIDTVELKGEGFKRIAEEGQRVKVGDTVIEFDLPLLEEKAKSTLTPVVIS 142
            +G E+ +H GIDTV + GEGF++   +G +VK GD +  FD   +        T V+++
Sbjct: 542 ANGAEILIHVGIDTVTMNGEGFEQKVAQGDKVKAGDVLGTFDSNKIAAAGLDDTTMVIVT 601

Query: 143 NMDEIKELIKL-SGSVTVGETPVIRIK 168
           N  +   +  + SGSV  G+  +I +K
Sbjct: 602 NTADYASVTPVASGSVVKGDA-IIEVK 627


Lambda     K      H
   0.315    0.138    0.374 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 242
Number of extensions: 12
Number of successful extensions: 1
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 169
Length of database: 628
Length adjustment: 28
Effective length of query: 141
Effective length of database: 600
Effective search space:    84600
Effective search space used:    84600
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (21.9 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory