Annotation: NCBI__GCF_000469325.1:WP_022528971.1
Length: 548 amino acids
Source: GCF_000469325.1 in NCBI
| Pathway | Step | Score | Similar to | Id. | Cov. | Bits | Other hit | Other id. | Other bits |
|---|---|---|---|---|---|---|---|---|---|
| trehalose catabolism | treC | med | trehalose-6-phosphate hydrolase (TreA;BSU07810) (EC 3.2.1.93) (characterized) | 47% | 98% | 528.1 | α-glucosidase (EC 3.2.1.20) | 46% | 526.2 |
| D-maltose catabolism | susB | med | α-glucosidase (EC 3.2.1.20) (characterized) | 46% | 99% | 526.2 | trehalose-6-phosphate hydrolase (TreA;BSU07810) (EC 3.2.1.93) | 47% | 528.1 |
| trehalose catabolism | treF | lo | α,α-trehalase / α-glucosidase (TTC0107) (EC 3.2.1.20|3.2.1.28) (characterized) | 40% | 69% | 273.9 | trehalose-6-phosphate hydrolase (TreA;BSU07810) (EC 3.2.1.93) | 47% | 528.1 |
| sucrose catabolism | ams | lo | Alpha-glucosidase; EC 3.2.1.20 (characterized, see rationale) | 37% | 70% | 261.2 | trehalose-6-phosphate hydrolase (TreA;BSU07810) (EC 3.2.1.93) | 47% | 528.1 |
View WP_022528971.1 at NCBI
Find papers: PaperBLAST
Find functional residues: SitesBLAST
Search for conserved domains
Find the best match in UniProt
Compare to protein structures
Predict transmenbrane helices: Phobius
Predict protein localization: PSORTb
Find homologs in fast.genomics
Fitness BLAST: loading...
MAWWHTATAYQIYPRSFQDSNGDGFGDIPGIIRRLPYLHELGIQLIWLTPMYPSPGYDNG YDISDYQAIDPRYGTLADFRALLTAAHQYNIRIMMDLVLNHTSSEHPWFRASRQDKTNPY ADFYFWTDTPTNWVSRFSGSTWQWDDTRQQYYLHIFEQHMPDLNWGNPMVRKKLLAMMAW WAEQGIDGFRLDVINMIAKRLPFGNVSRATPQDDGRAYYINGPRVHDYLRQMNDQVFGPH RMITVGELASAGVAETGAFTDPAHHELAMAFSFEHLKIDYTNGQKWTLGQFSLPALKAAL TKWQVGIAQAHGWNALFWTNHDQPRAISRFLNDDRYRTESQKLLAIVQFGLQGTPYIYQG EEIGMRNAYFTAIDQYDDRESRNAYAAMLAAGTDTATALAILQQKSRDNARTPMQWDDTV HHGFSTGTPWLAPGLPDPVTVQGALADPHSIFALYQHLISIRPQYAVFADGTFTMLAPDD TTVMHYQRENAATVLSVWANWSGTRQSRRAADLADAAILAHNYPDGLQLTQALLTLRPYE AVMVLRSK
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory