Align ABC transporter for L-Arginine and L-Citrulline, ATPase component (characterized)
to candidate WP_022529299.1 L248_RS06280 amino acid ABC transporter ATP-binding protein
Query= reanno::pseudo1_N1B4:Pf1N1B4_3435 (254 letters) >NCBI__GCF_000469325.1:WP_022529299.1 Length = 248 Score = 229 bits (585), Expect = 3e-65 Identities = 119/246 (48%), Positives = 167/246 (67%), Gaps = 11/246 (4%) Query: 4 LEVQDLHKRYGSHEVLKGVSLKAAAGDVISIIGSSGSGKSTFLRCINLLEQPHAGKILLN 63 L+VQ+LHK +G HEVLK ++L G+V+SIIG+SGSGKST LR INLLEQP G IL + Sbjct: 7 LQVQNLHKSFGDHEVLKDINLDVHKGEVMSIIGASGSGKSTLLRNINLLEQPTGGDILFH 66 Query: 64 NEELKLVANKDGALKAADPKQLQRMRSRLSMVFQHFNLWSHMTAMENIMEAPVHVLGMSK 123 + + +D + R+++ MVFQ FNL++++ ++N+M VL Sbjct: 67 EKSI-----------LSDDFDVTLYRAKVGMVFQSFNLFNNLNVLQNVMVGQTTVLKRDA 115 Query: 124 TEAREKAEHYLNKVGVAHRKDAYPGHMSGGEQQRVAIARALAMEPEVMLFDEPTSALDPE 183 A+ A L +VG+A +A P +SGG+QQRVAIARA AM+PEV+LFDEPTSALDPE Sbjct: 116 AAAKTHAMELLERVGMAPYVNAKPDQLSGGQQQRVAIARAEAMDPEVLLFDEPTSALDPE 175 Query: 184 LVGDVLKVMQALAQEGRTMVVVTHEMGFAREVSNQLVFLHKGVVEESGNPREVLVNPQSE 243 +V +VL M +LA G TM++VTHEM FAR+VS+ + F++ GV+ E+G P ++ +PQ + Sbjct: 176 MVDEVLNTMSSLAHTGLTMIIVTHEMQFARDVSDVVCFMNDGVLAETGTPEQIFEHPQEQ 235 Query: 244 RLQQFL 249 R Q+FL Sbjct: 236 RTQEFL 241 Lambda K H 0.317 0.131 0.366 Gapped Lambda K H 0.267 0.0410 0.140 Matrix: BLOSUM62 Gap Penalties: Existence: 11, Extension: 1 Number of Sequences: 1 Number of Hits to DB: 214 Number of extensions: 8 Number of successful extensions: 2 Number of sequences better than 1.0e-02: 1 Number of HSP's gapped: 1 Number of HSP's successfully gapped: 1 Length of query: 254 Length of database: 248 Length adjustment: 24 Effective length of query: 230 Effective length of database: 224 Effective search space: 51520 Effective search space used: 51520 Neighboring words threshold: 11 Window for multiple hits: 40 X1: 16 ( 7.3 bits) X2: 38 (14.6 bits) X3: 64 (24.7 bits) S1: 41 (21.7 bits) S2: 46 (22.3 bits)
This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.
Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.
A candidate for a step is "high confidence" if either:
Otherwise, a candidate is "medium confidence" if either:
Other blast hits with at least 50% coverage are "low confidence."
Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:
GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).
For more information, see:
If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know
by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory