GapMind for catabolism of small carbon sources

 

Alignments for a candidate for drdehyd-alpha in Amycolatopsis halophila YIM 93223

Align 2-deoxy-D-ribose dehydrogenase α subunit (characterized)
to candidate WP_157362518.1 AMYHA_RS26380 (2Fe-2S)-binding protein

Query= metacyc::MONOMER-20832
         (151 letters)



>NCBI__GCF_000504245.1:WP_157362518.1
          Length = 194

 Score =  136 bits (342), Expect = 2e-37
 Identities = 63/128 (49%), Positives = 87/128 (67%), Gaps = 1/128 (0%)

Query: 19  PLLWVIRDDLGLTGTKYGCGLAQCGACSVLVDGNVVRSCVTPVAGVVGREITTIEAIETD 78
           PLLWV+RD LG+TG KYGCG+  C AC+  ++GN ++ CV PV+   G+E+TTIE +   
Sbjct: 2   PLLWVLRDKLGITGPKYGCGVGLCRACTSHLNGNEIQPCVVPVSECDGQEVTTIEGLADG 61

Query: 79  EVGKRVVATWVEHQVAQCGYCQSGQVMAATALLKHTPAPSKAQIDAAMINLCRCGTYNAI 138
           +    V   WV+  V QCG+CQ GQ+MAA ALL+    P+ A ID A+ N+CRCGTY  +
Sbjct: 62  DELHPVQQAWVDADVGQCGFCQPGQIMAAVALLQRKSKPTDADID-AIENMCRCGTYPRL 120

Query: 139 HAAVDDLA 146
            AA+ ++A
Sbjct: 121 RAAIKEIA 128


Lambda     K      H
   0.320    0.134    0.410 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 129
Number of extensions: 4
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 151
Length of database: 194
Length adjustment: 18
Effective length of query: 133
Effective length of database: 176
Effective search space:    23408
Effective search space used:    23408
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 43 (21.2 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory