Alignments for a candidate for drdehyd-beta in Amycolatopsis halophila YIM 93223

GapMind for catabolism of small carbon sources

Alignments for a candidate for drdehyd-beta in Amycolatopsis halophila YIM 93223

Align 2-deoxy-D-ribose dehydrogenase β subunit (characterized)
to candidate WP_034270944.1 AMYHA_RS11850 xanthine dehydrogenase family protein molybdopterin-binding subunit

Query= metacyc::MONOMER-20833
         (739 letters)



>NCBI__GCF_000504245.1:WP_034270944.1
          Length = 727

 Score =  275 bits (704), Expect = 5e-78
 Identities = 235/757 (31%), Positives = 363/757 (47%), Gaps = 81/757 (10%)

Query: 14  LSRRRFL-----ASTAVGALVIGFGLPLGAGRVQAATSAE---------RGTQVPAF--- 56
           L RR+FL     A T   A   GF     A  V +  S E         R   +P     
Sbjct: 15  LKRRKFLTFLVAAPTLTVAGRFGFDALTEASAVPSLPSVEEIADLGEVIRLASMPTMHLM 74

Query: 57  -LEIRPDGTVRLLSPFMEGGQGTHTAMAQIVGEELDADPATFIVEAAPPGEAYVVMENGL 115
            LE+  D TVR   P +E GQG  T++A ++ EELDA      VE +         +   
Sbjct: 75  VLEVTEDTTVRFELPRLEVGQGISTSIAMVLAEELDARLDDVDVELSDSRPELHFNQ--- 131

Query: 116 RITGGSMSVRMSYPTMRRLGALARAMLLQAGAKQWGVPVGELTTQPGRVVHAASGRSLGY 175
            IT GS ++R+ +  +RR+ A ARA L+ A A QWG+P  +LTT+    VHA  GR+L Y
Sbjct: 132 -ITAGSTTMRVLWDPVRRMAADARARLVNAAANQWGLPAEQLTTKDS-AVHAPDGRTLSY 189

Query: 176 GELASSALDMP---VPDPASITLRDPSQFRWIGKPVKRLDAYDKSTGKALYSIDLKVDNM 232
           GEL+++A +      P PAS       ++  +G PV R DA D  TGKA Y++DL+++  
Sbjct: 190 GELSAAAREESGNGEPKPAS-------EYTVVGTPVARTDARDIVTGKAQYAMDLQIEGA 242

Query: 233 LHAAVQHAPRLGMTVGSLRNQSQVEGMKGVHSVHVLPGAVAVVAERWWHAKRAVEAIQVD 292
           L   V   P +  TVGS+ N ++   M GV ++  +P  VAV AE ++ A RA +A+++ 
Sbjct: 243 LPTVVARPPTIKGTVGSV-NDARARSMPGVVAITEIPTGVAVSAETFYDAMRARDALEIS 301

Query: 293 WQEAAADSALRVMPADFSSDKHREFLAAQQGPTRDDENEGDVAGALKGAKTQVEATYHNQ 352
           W+    D+          SD  ++            +N      A K     ++AT+   
Sbjct: 302 WKPGPIDTVS-------DSDIRKKL-----------KNVAHPLAAPKLRGRHLDATFDFA 343

Query: 353 YLNHAQLEPPSALARFNPDGTLEIWLPNQAPDMFRADIAKRTGLDPAQITLHSPLLGGFF 412
           ++NHA +E  SA+A    DGT E+W+P+Q     +  IAK  GL+   +++H    GG F
Sbjct: 344 FVNHAPMEVESAVADVRHDGTAEVWMPSQTAIWAQGAIAKELGLNRDAVSVHVTRAGGSF 403

Query: 413 GRHFLYDSANPYPQAIALAKAVSRPIKLIWSREEEFLRDVLRPLAVVKFRAALDDKGLPV 472
           GR    D      +A  +++A+ RP+KL+ +R+++     +RP    K RA+   KG  +
Sbjct: 404 GRRLFADVG---LEAARVSQAIGRPVKLMRTRQDDMKHGRMRPAYHQKIRASY-TKGEVL 459

Query: 473 AIE------AVSATEGPTEALAGKQGDKIDPTAVEGLSGKSYAIP------NKRIAQIYV 520
           + E       +    G  EAL       ++  A +     S ++P      N  + ++ +
Sbjct: 460 SFEHRVTGVGLDFDHGLGEALTSTLAQTVEGGASQYYFAVSQSMPYHVGVVNHTLQEVQL 519

Query: 521 KGAPMLGYWRSVGNSLNDFFYESFLDELADKGGHDPYELRLHLLRDNPRLTTLLQAAGEL 580
            G P  G +RSV +       E  +DELA++ G DP E R  + + +     L + A E+
Sbjct: 520 -GIP-TGSFRSVFSGTVRAAEEVVMDELAEQMGKDPLEFRREVAKADSGKAVLTKVA-EV 576

Query: 581 SGGWKRGPFTAEDGTRRARGVAMASPFGSHTAAIAEV-SIENGKVKVHDIWQAIDPGSIV 639
           SG  K  P         A GV   S + SH A + E+ + +    +V   + A D G ++
Sbjct: 577 SGWGKDMP------AGHALGVGYHSEYRSHAACVVEINATDPANPRVTKAFIAADVGQVI 630

Query: 640 NPAIVEAQVNGAVALGLSQTLLEEAVYVD-GKPRARNY-DLYPILAPAQMARVHVRVVES 697
           N   +EAQ+ GA   G++ T+L+  V++D G  R  +Y D           +  V ++ +
Sbjct: 631 NQKGLEAQLMGATMDGIA-TVLQAGVHIDNGAVRESSYGDFLWTKQGDSPTQFEVHLIRT 689

Query: 698 GEKMGGIGEPPLPAVAPAVANAVAQLTGQRVRSLPLS 734
               GG GE  LPA A AVANA A+ TG + RS P++
Sbjct: 690 DWAPGGAGELALPAAAGAVANAYAKATGTKPRSFPIN 726


Lambda     K      H
   0.317    0.134    0.394 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1264
Number of extensions: 72
Number of successful extensions: 10
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 739
Length of database: 727
Length adjustment: 40
Effective length of query: 699
Effective length of database: 687
Effective search space:   480213
Effective search space used:   480213
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 55 (25.8 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources