Alignments for a candidate for prpE in Amycolatopsis halophila YIM 93223

GapMind for catabolism of small carbon sources

Alignments for a candidate for prpE in Amycolatopsis halophila YIM 93223

Align Acetyl-coenzyme A synthetase, cytoplasmic; Acetate--CoA ligase; Acetyl-CoA synthetase; ACS; AceCS; Acetyl-CoA synthetase 1; AceCS1; Acyl-CoA synthetase short-chain family member 2; Acyl-activating enzyme; Propionate--CoA ligase; EC 6.2.1.1; EC 6.2.1.17 (characterized)
to candidate WP_034268252.1 AMYHA_RS04515 acetoacetate--CoA ligase

Query= SwissProt::Q9QXG4
         (701 letters)



>NCBI__GCF_000504245.1:WP_034268252.1
          Length = 674

 Score =  193 bits (490), Expect = 3e-53
 Identities = 177/647 (27%), Positives = 275/647 (42%), Gaps = 56/647 (8%)

Query: 37  RSAHVPSLQRYRELHRRSVEEPREFWGNIAKEFYWKTACPGPFLQYNFDVTKGKIFTE-- 94
           R A    +  Y EL   SV E   FW + A EF     C   + +    +T+G+   +  
Sbjct: 36  RDAKDVQVNDYDELWEWSVTELDAFW-SAAAEF-----CGVRWHERPTAITEGERMPDVH 89

Query: 95  WMKGATTNICYNVLDRNVH-EKKLGDKVAFYWEGNEPGETTKITYRELLVQVCQFSNVLR 153
           W  G T N   + L   V   +K  D +A  +   E G T ++TY +L  QV      L 
Sbjct: 90  WFPGGTLNYAEHALSGGVAGAEKRDDDLAVIFH-REDGSTEQLTYGQLRSQVAAARAALS 148

Query: 154 KQGIQKGDRVAIYMPMILELVVAMLACARLGALHSIVFAGFSAESLCERILDSSCCLLIT 213
             G+  GDRV    P   + ++A LA A LGA+ S     F   ++ +R       +L+ 
Sbjct: 149 SLGVGMGDRVVALAPNCPQTLIAFLAAASLGAVWSSCSPDFGVRAIADRFTQIEPKVLVA 208

Query: 214 TDAFYRGEKLVNLKELADESLEKCREKGFPVRCCIVVKHLGRAELGMNDSPSQSPPVKRP 273
            + +  G +  ++     E                      RAE+     PS +  V   
Sbjct: 209 VNGYMYGGRTFDITGTVREL---------------------RAEM-----PSLAGTVLID 242

Query: 274 CPDVQICWNEGVDL---WWHELMQQAGDECEPEWCDAEDPLFILYTSGSTGKPKGVVHTI 330
             D       GVD    W   L Q  G     +    E PL++LY+SG+TG PKG+V   
Sbjct: 243 YADTG-----GVDDTHDWDALLAQHDGAPLAFDPVPFEHPLWVLYSSGTTGLPKGIVQGH 297

Query: 331 GGYMLYVATTFKYVFDFHPEDVFWCTADIGWITGHSYVTYGPLANGATSVLFEGIPTYPD 390
           GG  L    +     D  P + F+     GW+  +  ++   L  G+T VL++G P YPD
Sbjct: 298 GGITLEHLKSLSLQMDLGPGERFFWFTTTGWMMWNFLIS--GLLVGSTVVLYDGSPGYPD 355

Query: 391 EGRLWSIVDKYKVTKFYTAPTAIRMLMKFGDDPVTKHSRASLQVLGTVGEPINPEAWLWY 450
            G LW + +++++T F  +   I+   K    P T+H  ++L+ +G+ G P++ E + W 
Sbjct: 356 LGALWRLAEQHRITYFGVSAPFIQQCFKQRLSPTTEHDLSALRAVGSTGAPLSVEGFRWI 415

Query: 451 HRVVGSQRCPIVDTFWQTETGGHMLTPLPGATPMKP---GSASFPFFGVAPAILNESGEE 507
              +G               G  + T   GA+P  P   G  S    G A A  +E G E
Sbjct: 416 IDELGRDEHGRPVQICSVSGGTDVCTAFVGASPDVPVWLGELSCRALGAAVAAFDEDGNE 475

Query: 508 LEGEAEGYLVFKQPWPGIMRTVYGNH---TRFETTYFKKFPGYYVTGDGCRRDQDGYYWI 564
           +  E  G LV  +P P  M   + N     R  + YF+++PG +  GD  R    G   I
Sbjct: 476 VV-EQVGELVITKPMPS-MPVFFWNDADGARLRSAYFEEYPGVWRHGDWIRITSRGSSVI 533

Query: 565 TGRIDDMLNVSGHLLSTAEVESALVEHEAVAEAAVVGHPHPVKGE-CLYCFVTLCDGHTF 623
            GR D  LN  G  + TAE    +   + +A++ V+      + E  L CFV L  G + 
Sbjct: 534 YGRSDSTLNRGGVRMGTAEFYRVVEGRDEIADSLVIDTTRVGEDEGKLLCFVVLAPGASL 593

Query: 624 SPTLTEELKKQIREKIGPIATPDYIQNAPGLPKTRSGKIMRRVLRKI 670
           +  +  +L+K++R  + P   PD       +P+T +GK     ++KI
Sbjct: 594 A-DVEPDLRKELRSALSPRHVPDEFIEVSDVPRTLNGKKCEVPVKKI 639


Lambda     K      H
   0.320    0.138    0.444 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 1255
Number of extensions: 57
Number of successful extensions: 5
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 701
Length of database: 674
Length adjustment: 39
Effective length of query: 662
Effective length of database: 635
Effective search space:   420370
Effective search space used:   420370
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 54 (25.4 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Rules (tab-delimited)
Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources