GapMind for catabolism of small carbon sources

 

Protein WP_024771487.1 in Aquimarina macrocephali JAMB N27

Annotation: NCBI__GCF_000520995.1:WP_024771487.1

Length: 478 amino acids

Source: GCF_000520995.1 in NCBI

Candidate for 14 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
D-cellobiose catabolism MFS-glucose hi Glucose/galactose porter (characterized) 44% 98% 315.8 D-mannitol and D-mannose transporter (MFS superfamily) 44% 258.5
D-galactose catabolism HP1174 hi Glucose/galactose porter (characterized) 44% 98% 315.8 D-mannitol and D-mannose transporter (MFS superfamily) 44% 258.5
D-glucose catabolism MFS-glucose hi Glucose/galactose porter (characterized) 44% 98% 315.8 D-mannitol and D-mannose transporter (MFS superfamily) 44% 258.5
lactose catabolism MFS-glucose hi Glucose/galactose porter (characterized) 44% 98% 315.8 D-mannitol and D-mannose transporter (MFS superfamily) 44% 258.5
D-maltose catabolism MFS-glucose hi Glucose/galactose porter (characterized) 44% 98% 315.8 D-mannitol and D-mannose transporter (MFS superfamily) 44% 258.5
sucrose catabolism MFS-glucose hi Glucose/galactose porter (characterized) 44% 98% 315.8 D-mannitol and D-mannose transporter (MFS superfamily) 44% 258.5
trehalose catabolism MFS-glucose hi Glucose/galactose porter (characterized) 44% 98% 315.8 D-mannitol and D-mannose transporter (MFS superfamily) 44% 258.5
D-mannose catabolism gluP med The Na+-dependent sugar transporter, HP1174 (transports glucose, galactose, mannose and 2-deoxyglucose (Psakis et al. 2009)). (most similar to 2.A.1.7.2; 49% identity) (characterized) 43% 80% 261.2 Glucose/galactose porter 44% 315.1
D-fructose catabolism fruP lo MFS transporter, FHS family, L-fucose permease (characterized, see rationale) 37% 92% 254.2 Glucose/galactose porter 44% 315.1
sucrose catabolism fruP lo MFS transporter, FHS family, L-fucose permease (characterized, see rationale) 37% 92% 254.2 Glucose/galactose porter 44% 315.1
L-fucose catabolism fucP lo L-fucose-proton symporter; 6-deoxy-L-galactose permease; L-fucose permease (characterized) 31% 79% 163.7 Glucose/galactose porter 44% 315.1
2-deoxy-D-ribose catabolism deoP lo 2-Deoxy-D-ribose porter, DeoP (characterized) 30% 72% 145.6 Glucose/galactose porter 44% 315.1
N-acetyl-D-glucosamine catabolism nagP lo N-acetylglucosamine porter, NagP (characterized) 31% 73% 142.1 Glucose/galactose porter 44% 315.1
D-glucosamine (chitosamine) catabolism nagP lo N-acetylglucosamine porter, NagP (characterized) 31% 73% 142.1 Glucose/galactose porter 44% 315.1

Sequence Analysis Tools

View WP_024771487.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MSDITNSLLTKSERQNNYNKPFFAIIFLFFLWGFITVMNDVLIPHLKAVFELSYFQAALI
QFAFFGAFFIISLIYFITSVSIGDPISKIGYKNGIIIGLILCGIGCCLFYPAASYQRYAI
FLGALFVLASGVTILQITANPYAAILGKPETAPSRLNLAQGFNSFGTTLAPIVGAILLYK
VFSDGEITVDSLKMPYLIYGSLFFVLAIIIKFVKLPSFINNEKIEKGLGVLKFRHVVLGM
FAIFFYVGGEVSIGSFLINFFGLETVMGMKEAEAGVFLSYYWGGAMIGRFLGSVSMSGIT
STRKKYLLMALLSVLFFFVLYFITAIKIEAGTFSLQFLSFSKIWLFVVFLISNYLFFLIG
GTKPSKVLTLFVGIIIVLLLIMILCNGEIAFWSAIAIGAFNSILWSNIFTLAIKDLGKYT
SQASSLLVMMIVGGALIPLLQGVVADYIGIKLSFFIPVICYGYLIFYGLVGYKVKNTL

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory