Alignments for a candidate for cbtF in Aquimarina macrocephali JAMB N27

GapMind for catabolism of small carbon sources

Alignments for a candidate for cbtF in Aquimarina macrocephali JAMB N27

Align CbtF, component of Cellobiose and cellooligosaccharide porter (characterized)
to candidate WP_024769669.1 Z054_RS0108590 ABC transporter ATP-binding protein

Query= TCDB::Q97VF4
         (324 letters)



>NCBI__GCF_000520995.1:WP_024769669.1
          Length = 563

 Score =  184 bits (468), Expect = 3e-51
 Identities = 100/262 (38%), Positives = 159/262 (60%), Gaps = 8/262 (3%)

Query: 3   LMELKGVSVIFEDKVGLFKKRKFYALKDVSLSMNQGDLLIVLGESGAGKTTLGRVIVGLQ 62
           L+E+  V   +  KVGLF K +F A+ DVS  + +G+ L ++GESG GK+TLG  I+ L 
Sbjct: 306 LLEVINVEKSYFSKVGLFGKAEFKAVDDVSFKLYEGETLGLVGESGCGKSTLGNTILQLD 365

Query: 63  KPTSGEVVYDGYNIWKNKRKIFKKYRKDVQLIPQDPYSTLPFNKTVEEILVAPILRWEKI 122
           K   G+V+Y G +I +      +  RK++QLI QDP+++L     V + ++ P+      
Sbjct: 366 KANRGQVIYKGQDITQLSSSEIRSLRKEIQLIFQDPFASLNPRLAVGKAIMEPMQAHNLY 425

Query: 123 NKDELRK-RLINLLELVKLTPAEEFLGKYPHQLSGGQKQRLSIARSLSVNPRIIVADEPV 181
           N D+ RK ++I LLE V LT  EE   +YPH+ SGGQ+QR+ IAR++++ P++I+ DE V
Sbjct: 426 NNDKERKQKVIELLERVSLT--EEHFNRYPHEFSGGQRQRIGIARTIALQPKLIICDESV 483

Query: 182 TMVDASLRIGILNTLAEIKNRLNLTMVFITHDIPIARYFYHLFDKGNTIVMFAGRIVERA 241
           + +D S++  +LN L E+K+    T +FI+HD+ + +Y          +VM  G+I E+ 
Sbjct: 484 SALDISVQAQVLNLLNELKSNFGFTYIFISHDLAVVKYM-----SDQLLVMNKGKIEEQG 538

Query: 242 DLEEILKDPLHPYTNDLIKLTP 263
           D + I ++P   YT  LI+  P
Sbjct: 539 DADLIYENPQKEYTKKLIQAIP 560



 Score =  136 bits (343), Expect = 1e-36
 Identities = 73/260 (28%), Positives = 145/260 (55%), Gaps = 11/260 (4%)

Query: 19  LFKKRKFYALKDVSLSMNQGDLLIVLGESGAGKTTLGRVIVGL-----QKPTSGEVVYDG 73
           L ++++   L D+S  +++ ++L V+GESG+GK+     I+GL      + T G+++YDG
Sbjct: 17  LSEEKENQVLYDISFDIHKNEILGVVGESGSGKSVASLAILGLLPNKISRITKGDIIYDG 76

Query: 74  YNIWKNKRKIFKKYR-KDVQLIPQDPYSTLPFNKTVEEILVAPILRWEKINKDELRKRLI 132
            ++     K ++  R  ++ +I Q+P S+L  +      +   + +   ++K E++  ++
Sbjct: 77  VSLSNFNEKEYRAIRGNEIAMIFQEPMSSLNPSMKCGRQVAEILFQHTSLSKTEIKTEVL 136

Query: 133 NLLELVKLTPAEEFLGKYPHQLSGGQKQRLSIARSLSVNPRIIVADEPVTMVDASLRIGI 192
           +L E VKL   +     YPHQLSGGQKQR+ IA +++  P++++ADEP T +D +++  I
Sbjct: 137 SLFEKVKLPEPDRAYKSYPHQLSGGQKQRIMIAMAIACKPKLLIADEPTTALDVTVQKEI 196

Query: 193 LNTLAEIKNRLNLTMVFITHDIPIARYFYHLFDKGNTIVMFAGRIVERADLEEILKDPLH 252
           +  L  ++    ++++FI+HD+ +           + +VM+ G+++E      I   P  
Sbjct: 197 IGLLKSLQEEYKMSILFISHDLSLVSEV-----ADHVLVMYQGKMIEYGTTNTIFHTPQK 251

Query: 253 PYTNDLIKLTPSIDNLYKEI 272
            YT  LI   P ++  Y+++
Sbjct: 252 EYTKALINARPPMNIRYQKL 271


Lambda     K      H
   0.321    0.141    0.410 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 500
Number of extensions: 20
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 324
Length of database: 563
Length adjustment: 32
Effective length of query: 292
Effective length of database: 531
Effective search space:   155052
Effective search space used:   155052
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Protein sequences (fasta format)
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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources