GapMind for catabolism of small carbon sources

 

Alignments for a candidate for PS417_17605 in Dyadobacter tibetensis Y620-1

Align ATP-binding cassette domain-containing protein; SubName: Full=Amino acid transporter; SubName: Full=Histidine ABC transporter ATP-binding protein; SubName: Full=Histidine transport system ATP-binding protein (characterized, see rationale)
to candidate WP_025761778.1 X939_RS0102915 ABC transporter ATP-binding protein

Query= uniprot:A0A1N7U8S3
         (276 letters)



>NCBI__GCF_000566685.1:WP_025761778.1
          Length = 321

 Score =  129 bits (323), Expect = 1e-34
 Identities = 78/251 (31%), Positives = 140/251 (55%), Gaps = 16/251 (6%)

Query: 27  LQVEGIHKRYGEHEVLKGVSLNARQGDVISLIGASGSGKSTMLRCINFLEQPDAGVITLD 86
           +QV  + K YG  + +  +S  A+ G    L+G SGSGK+T L+ IN L +P  G I +D
Sbjct: 2   IQVNRVSKHYGSIKAVNRISFEAKLGSTTVLVGTSGSGKTTTLKTINRLIEPSEGQILID 61

Query: 87  GISIEMRQGRAGTRAPHQDQLQNLRTRLAMVFQHFNLWSHMTVLENITMAPRRVLDVSAA 146
           G  I +++             Q LR ++  V Q+  L+ H TV +N+ + P  ++  SA 
Sbjct: 62  GKDISLQKP------------QLLRRQIGYVSQNNGLFPHYTVRQNVAIVP-SLIGWSAE 108

Query: 147 EAEKRARMYLDKVGLP-SRVADQYPAFLSGGQQQRVAIARALAMEPEIILFDEPTSALDP 205
           +  +R+   L+++ +P ++   ++P  LSGGQ QRVA+ARAL   P ++L DEP  ALDP
Sbjct: 109 KTLQRSEEILEQLLIPMAQFGHKFPKELSGGQYQRVALARALVTRPPVLLMDEPFGALDP 168

Query: 206 ELVGEVLKVIQTL-AEEGRTMLMVTHEMGFARQVSSQVLFLHQGRVEEHGDAR-ILDQPN 263
               ++ + +++L     R++++VTH++  A ++  Q++ + +G+  + G A  +L +P 
Sbjct: 169 VTRQQIRQELKSLPGHPERSIVLVTHDLAEAIELGDQIVVMDRGKCLQQGPASDLLFKPR 228

Query: 264 SERLQQFLSNR 274
              ++ F   +
Sbjct: 229 DSFIKSFFDTQ 239


Lambda     K      H
   0.319    0.133    0.372 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 181
Number of extensions: 11
Number of successful extensions: 4
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 276
Length of database: 321
Length adjustment: 26
Effective length of query: 250
Effective length of database: 295
Effective search space:    73750
Effective search space used:    73750
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 48 (23.1 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory