GapMind for catabolism of small carbon sources

 

Alignments for a candidate for bztD in Skermanella stibiiresistens SB22

Align BztD, component of Glutamate/glutamine/aspartate/asparagine porter (characterized)
to candidate WP_157619716.1 N825_RS33165 ATP-binding cassette domain-containing protein

Query= TCDB::Q52666
         (263 letters)



>NCBI__GCF_000576635.1:WP_157619716.1
          Length = 243

 Score =  258 bits (658), Expect = 1e-73
 Identities = 134/240 (55%), Positives = 171/240 (71%), Gaps = 2/240 (0%)

Query: 23  IQISQMNKWYGQFHVLRDINLTVHRGERIVIAGPSGSGKSTMIRCINRLEEHQSGKIIVD 82
           I+ S +NK YG F  L DIN  V RGE +V+ GPSGSGKST+IR +NRLEE  SG I  D
Sbjct: 2   IRFSNVNKNYGGFQALVDINAEVARGEVVVVCGPSGSGKSTLIRTVNRLEEIHSGTITFD 61

Query: 83  GIELTSDLKN--IDKVRSEVGMVFQHFNLFPHLTILENLTLAPIWVRKVPKREAEETAMY 140
           G  +   ++   ++ +RS +G VFQ FNLFPHL+ LEN+T++P+ V  V + +A + AM 
Sbjct: 62  GRSVHGKMRGSELNHLRSRIGFVFQSFNLFPHLSALENVTISPVKVNGVDREQARDRAMR 121

Query: 141 YLEKVKIPEQAQKYPGQLSGGQQQRVAIARSLCMKPKIMLFDEPTSALDPEMIKEVLDTM 200
            L++V +  +A  YP QLSGGQQQRVAIAR+L M+P  MLFDEPTSALDPEM+ EVL  M
Sbjct: 122 LLDRVGLSAKANAYPAQLSGGQQQRVAIARALAMEPPAMLFDEPTSALDPEMVGEVLAVM 181

Query: 201 IQLAEEGMTMLCVTHEMGFAQAVANRVIFMADGQIVEQNNPHDFFHNPQSERTKQFLSQI 260
             LA +GMTM+CVTHEMGFA+ VA+RV FM  GQI+E   P  FF +PQ  R ++FLS +
Sbjct: 182 RGLAADGMTMMCVTHEMGFAREVADRVWFMDAGQILETAEPSVFFGDPQHARAQRFLSDL 241


Lambda     K      H
   0.320    0.134    0.391 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 187
Number of extensions: 7
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 263
Length of database: 243
Length adjustment: 24
Effective length of query: 239
Effective length of database: 219
Effective search space:    52341
Effective search space used:    52341
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory