Alignments for a candidate for PS417_12065 in Skermanella stibiiresistens SB22

GapMind for catabolism of small carbon sources

Alignments for a candidate for PS417_12065 in Skermanella stibiiresistens SB22

Align D-ribose transporter ATP-binding protein; SubName: Full=Putative xylitol transport system ATP-binding protein; SubName: Full=Sugar ABC transporter ATP-binding protein (characterized, see rationale)
to candidate WP_037446034.1 N825_RS01525 sugar ABC transporter ATP-binding protein

Query= uniprot:A0A1N7TX47
         (495 letters)



>NCBI__GCF_000576635.1:WP_037446034.1
          Length = 496

 Score =  366 bits (940), Expect = e-106
 Identities = 203/497 (40%), Positives = 302/497 (60%), Gaps = 11/497 (2%)

Query: 6   LLQAEHVAKAYAGVPALRDGRLSLRAGSVHALCGGNGAGKSTFLSILMGITQRDAGSILL 65
           +L    V+K++ GV AL D RL LRAG V AL G NGAGKST + IL GI + DAG I +
Sbjct: 1   MLALAGVSKSFPGVKALDDVRLELRAGEVMALVGENGAGKSTLVKILTGIERPDAGGITI 60

Query: 66  NGAPVQFNRPSEALAAGIAMITQELEPIPYMTVAENIWLGREP-------RRAGCIVDNK 118
           +G+PV F  P +A  AGI  I QE      +TVAENI +G  P       RR    VD +
Sbjct: 61  DGSPVSFGSPHDAWLAGITAIHQETVMFDDLTVAENIHMGHHPTLGRGPFRR---FVDWR 117

Query: 119 ALNRRTRELLDSLEFDVDATSPMHRLSVAQIQLVEIAKAFSHDCQVMIMDEPTSAIGEHE 178
           A+  RTR +L  LE D+   + +  L+VA+  +V IAKA SHD +++IMDEPT+A+ +HE
Sbjct: 118 AMKARTRAVLARLEVDIQPDTVLRDLTVARKHIVGIAKALSHDARIVIMDEPTAALSQHE 177

Query: 179 AQTLFKAIRRLTAQGAGIVYVSHRLSELAQIADDYSIFRDGAFVESGRMADIDRDHLVRG 238
              L+K + +L A G  I+++SH+  E+  IAD +++ RDG F+ +G + ++ RD LV  
Sbjct: 178 IAELYKIVGQLKAAGKAILFISHKFDEIFAIADRWTVLRDGRFIGAGAIGEVTRDELVTM 237

Query: 239 IVGQELTRIDHKVGRECAANTCLQVDNLSRAGEFHDISLQLRQGEILGIYGLMGSGRSEF 298
           +VG+ L ++  K+       T L+V++LS   EF  I  +LR+GEILG YGL+G+GRSE 
Sbjct: 238 MVGRRLDQVFPKITVPLG-ETVLEVNDLSHPTEFDGIGFKLRRGEILGFYGLVGAGRSEA 296

Query: 299 LNCIYGLTVADSGSVTLQGKPMPIGLPKATINAGMSLVTEDRKDSGLVLTGSILSNIALS 358
           +  ++G+T    G V + G+ + +  P   I AG++ V EDR+  G +L   I  NI L 
Sbjct: 297 MQALFGITKPSRGRVRIAGRDVVVRNPSDAIRAGIAYVPEDRQVQGAILPMGITENITLP 356

Query: 359 AYKRLSSWSLINARKETQLAEDMVKRLQIKTTSLELPVASMSGGNQQKVVLAKCLSTEPV 418
              R++   ++   +E  +A    +RL +K T  + PV ++SGGNQQKV++AK L+T P 
Sbjct: 357 VVDRVNGGPVLRPARERAVARRFGERLAVKATHWDQPVGTLSGGNQQKVLIAKWLATNPA 416

Query: 419 CLLCDEPTRGIDEGAKQEIYHLLDQFVRGGGAAIVVSSEAPELLHLSDRIAVFKGGRLVT 478
            ++ DEPT+GID G+K  ++  + + VR G A I+VSSE PE++ +SD I V   GR+  
Sbjct: 417 VIILDEPTKGIDVGSKAAVHEFMGELVRSGLAVILVSSELPEIMGMSDTIIVMHEGRIRR 476

Query: 479 ISTDTALSQEALLRLAS 495
             +    + EA++  A+
Sbjct: 477 RFSRAEATAEAIVTAAT 493



 Score = 71.6 bits (174), Expect = 6e-17
 Identities = 62/219 (28%), Positives = 93/219 (42%), Gaps = 7/219 (3%)

Query: 29  LRAGSVHALCGGNGAGKSTFLSILMGITQRDAGSILLNGAPVQFNRPSEALAAGIAMITQ 88
           LR G +    G  GAG+S  +  L GIT+   G + + G  V    PS+A+ AGIA + +
Sbjct: 277 LRRGEILGFYGLVGAGRSEAMQALFGITKPSRGRVRIAGRDVVVRNPSDAIRAGIAYVPE 336

Query: 89  ELE---PIPYMTVAENIWLGREPRRAGCIVDNKALNRRTRELLDSLEFDVDAT---SPMH 142
           + +    I  M + ENI L    R  G  V   A  R            V AT    P+ 
Sbjct: 337 DRQVQGAILPMGITENITLPVVDRVNGGPVLRPARERAVARRFGE-RLAVKATHWDQPVG 395

Query: 143 RLSVAQIQLVEIAKAFSHDCQVMIMDEPTSAIGEHEAQTLFKAIRRLTAQGAGIVYVSHR 202
            LS    Q V IAK  + +  V+I+DEPT  I       + + +  L   G  ++ VS  
Sbjct: 396 TLSGGNQQKVLIAKWLATNPAVIILDEPTKGIDVGSKAAVHEFMGELVRSGLAVILVSSE 455

Query: 203 LSELAQIADDYSIFRDGAFVESGRMADIDRDHLVRGIVG 241
           L E+  ++D   +  +G        A+   + +V    G
Sbjct: 456 LPEIMGMSDTIIVMHEGRIRRRFSRAEATAEAIVTAATG 494


Lambda     K      H
   0.319    0.135    0.381 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 605
Number of extensions: 24
Number of successful extensions: 7
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 2
Number of HSP's successfully gapped: 2
Length of query: 495
Length of database: 496
Length adjustment: 34
Effective length of query: 461
Effective length of database: 462
Effective search space:   212982
Effective search space used:   212982
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.4 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.8 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources