GapMind for catabolism of small carbon sources

 

Alignments for a candidate for AO353_03040 in Bacillus altitudinis 41KF2b

Align ABC transporter for L-Arginine and L-Citrulline, ATPase component (characterized)
to candidate WP_007501456.1 BA79_RS06410 amino acid ABC transporter ATP-binding protein

Query= reanno::pseudo1_N1B4:Pf1N1B4_3435
         (254 letters)



>NCBI__GCF_000691145.1:WP_007501456.1
          Length = 242

 Score =  251 bits (640), Expect = 1e-71
 Identities = 130/245 (53%), Positives = 170/245 (69%), Gaps = 13/245 (5%)

Query: 7   QDLHKRYGSHEVLKGVSLKAAAGDVISIIGSSGSGKSTFLRCINLLEQPHAGKILLNNEE 66
           ++++K YG   VLK ++L    G+V+ IIG SGSGKST LRCIN LE    GK+L+N   
Sbjct: 5   EEVNKHYGQFHVLKDINLHIQKGEVVVIIGPSGSGKSTMLRCINRLESIDEGKVLVNQVS 64

Query: 67  LKLVANKDGALKAADPK-QLQRMRSRLSMVFQHFNLWSHMTAMENIMEAPVHVLGMSKTE 125
           ++             PK  +  +R  + MVFQHF+L+ H T +ENIM AP+ V  +SK E
Sbjct: 65  VQ------------HPKTNINLIRQNIGMVFQHFHLYPHKTVLENIMLAPMKVKKVSKEE 112

Query: 126 AREKAEHYLNKVGVAHRKDAYPGHMSGGEQQRVAIARALAMEPEVMLFDEPTSALDPELV 185
           A+E AE YLNKVG+  + D YP  +SGG+QQRVAIAR LAM+PEVMLFDEPTSALDPE++
Sbjct: 113 AKETAEFYLNKVGIPEKADTYPSRLSGGQQQRVAIARGLAMKPEVMLFDEPTSALDPEMI 172

Query: 186 GDVLKVMQALAQEGRTMVVVTHEMGFAREVSNQLVFLHKGVVEESGNPREVLVNPQSERL 245
           G+VL VM+ LA+EG TMVVVTHEMGFAREV++++VF+ +G + E   P      P+ +R 
Sbjct: 173 GEVLDVMKQLAREGMTMVVVTHEMGFAREVADRIVFIDEGRILEESTPAAFYEKPREKRA 232

Query: 246 QQFLS 250
           Q FLS
Sbjct: 233 QLFLS 237


Lambda     K      H
   0.317    0.131    0.366 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 207
Number of extensions: 6
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 254
Length of database: 242
Length adjustment: 24
Effective length of query: 230
Effective length of database: 218
Effective search space:    50140
Effective search space used:    50140
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory