GapMind for catabolism of small carbon sources

 

Alignments for a candidate for hpcD in Bacillus altitudinis 41KF2b

Align 3-hydroxypropionyl-CoA dehydratase (EC 4.2.1.116) (characterized)
to candidate WP_035701941.1 BA79_RS07305 enoyl-CoA hydratase

Query= BRENDA::A4YI89
         (259 letters)



>NCBI__GCF_000691145.1:WP_035701941.1
          Length = 259

 Score =  208 bits (530), Expect = 8e-59
 Identities = 114/245 (46%), Positives = 162/245 (66%), Gaps = 3/245 (1%)

Query: 17  ITLNRPDKLNALNAKLLEELDRAVSQAESDPEIRVIIITGKGKAFCAGADITQFNQLTPA 76
           IT+  P   NAL+++LL EL+    Q E + E+R I+I G+G+ F AGADI +F  L   
Sbjct: 16  ITIQHPPA-NALSSQLLTELNDMFDQLEQNSEVRAIVIHGEGRFFSAGADIKEFTTLQEE 74

Query: 77  EAW-KFSKKGREIMDKIEALSKPTIAMINGYALGGGLELALACDIRIAAEEAQLGLPEIN 135
             +   + +G+++ ++IE   KP IA I+G ALGGGLELA++CDIRIA ++A+LGLPE+N
Sbjct: 75  SDYASLADRGQQVFERIEQCPKPVIASIHGAALGGGLELAMSCDIRIATKDAKLGLPELN 134

Query: 136 LGIYPGYGGTQRLTRVIGKGRALEMMMTGDRIPGKDAEKYGLVNRVVPLANLEQETRK-L 194
           LGI PG+GGTQRL R +G  +ALEMM T + I G++A   GLV+++    +   ET K L
Sbjct: 135 LGIIPGFGGTQRLPRYVGSAKALEMMGTAEPITGEEAFACGLVSKLAETEDEALETAKTL 194

Query: 195 AEKIAKKSPISLALIKEVVNRGLDSPLLSGLALESVGWGVVFSTEDKKEGVSAFLEKREP 254
           A K A KSP +L  + E++N         G+ LE+  +G VF + D KEG+ AF+EKR+P
Sbjct: 195 AAKFAAKSPKTLEYVLELLNATKVYSYDGGMKLEAKKFGEVFQSNDAKEGIQAFIEKRKP 254

Query: 255 TFKGK 259
            FKG+
Sbjct: 255 NFKGE 259


Lambda     K      H
   0.315    0.135    0.382 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 153
Number of extensions: 5
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 259
Length of database: 259
Length adjustment: 24
Effective length of query: 235
Effective length of database: 235
Effective search space:    55225
Effective search space used:    55225
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 42 (22.0 bits)
S2: 47 (22.7 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory