GapMind for catabolism of small carbon sources

 

Protein WP_027699770.1 in Weissella oryzae SG25

Annotation: NCBI__GCF_000691805.2:WP_027699770.1

Length: 270 amino acids

Source: GCF_000691805.2 in NCBI

Candidate for 10 steps in catabolism of small carbon sources

Pathway Step Score Similar to Id. Cov. Bits Other hit Other id. Other bits
D-mannose catabolism manY hi Mannose-specific PTS system, IIC component, component of Mannose enzyme II complex, IIAB, IIC, IID. IIC/IID serve allows entry of some bacteriocins including pediocin (class IIa) and lactococcin A (class IIc) (Kjos et al., 2011). Transports and phosphorylates Glucose, Mannose and Glucosamine (characterized) 73% 99% 386.3 PTS system sorbose-specific EIIC component; EIIC-Sor; Sorbose permease IIC component 43% 217.6
D-cellobiose catabolism manY med PTS system, mannose/fructose/sorbose family, IIC component, component of The primary glucose /mannose uptake transporter, ManLMN (characterized) 59% 100% 308.9 Mannose-specific PTS system, IIC component, component of Mannose enzyme II complex, IIAB, IIC, IID. IIC/IID serve allows entry of some bacteriocins including pediocin (class IIa) and lactococcin A (class IIc) (Kjos et al., 2011). Transports and phosphorylates Glucose, Mannose and Glucosamine 73% 386.3
D-glucose catabolism manY med PTS system, mannose/fructose/sorbose family, IIC component, component of The primary glucose /mannose uptake transporter, ManLMN (characterized) 59% 100% 308.9 Mannose-specific PTS system, IIC component, component of Mannose enzyme II complex, IIAB, IIC, IID. IIC/IID serve allows entry of some bacteriocins including pediocin (class IIa) and lactococcin A (class IIc) (Kjos et al., 2011). Transports and phosphorylates Glucose, Mannose and Glucosamine 73% 386.3
lactose catabolism manY med PTS system, mannose/fructose/sorbose family, IIC component, component of The primary glucose /mannose uptake transporter, ManLMN (characterized) 59% 100% 308.9 Mannose-specific PTS system, IIC component, component of Mannose enzyme II complex, IIAB, IIC, IID. IIC/IID serve allows entry of some bacteriocins including pediocin (class IIa) and lactococcin A (class IIc) (Kjos et al., 2011). Transports and phosphorylates Glucose, Mannose and Glucosamine 73% 386.3
D-maltose catabolism manY med PTS system, mannose/fructose/sorbose family, IIC component, component of The primary glucose /mannose uptake transporter, ManLMN (characterized) 59% 100% 308.9 Mannose-specific PTS system, IIC component, component of Mannose enzyme II complex, IIAB, IIC, IID. IIC/IID serve allows entry of some bacteriocins including pediocin (class IIa) and lactococcin A (class IIc) (Kjos et al., 2011). Transports and phosphorylates Glucose, Mannose and Glucosamine 73% 386.3
sucrose catabolism manY med PTS system, mannose/fructose/sorbose family, IIC component, component of The primary glucose /mannose uptake transporter, ManLMN (characterized) 59% 100% 308.9 Mannose-specific PTS system, IIC component, component of Mannose enzyme II complex, IIAB, IIC, IID. IIC/IID serve allows entry of some bacteriocins including pediocin (class IIa) and lactococcin A (class IIc) (Kjos et al., 2011). Transports and phosphorylates Glucose, Mannose and Glucosamine 73% 386.3
trehalose catabolism manY med PTS system, mannose/fructose/sorbose family, IIC component, component of The primary glucose /mannose uptake transporter, ManLMN (characterized) 59% 100% 308.9 Mannose-specific PTS system, IIC component, component of Mannose enzyme II complex, IIAB, IIC, IID. IIC/IID serve allows entry of some bacteriocins including pediocin (class IIa) and lactococcin A (class IIc) (Kjos et al., 2011). Transports and phosphorylates Glucose, Mannose and Glucosamine 73% 386.3
D-fructose catabolism levF med Mannose-specific PTS system component IIC, component of The hexose (glucose and fructose demonstrated) PTS uptake system (characterized) 57% 100% 308.5 Mannose-specific PTS system, IIC component, component of Mannose enzyme II complex, IIAB, IIC, IID. IIC/IID serve allows entry of some bacteriocins including pediocin (class IIa) and lactococcin A (class IIc) (Kjos et al., 2011). Transports and phosphorylates Glucose, Mannose and Glucosamine 73% 386.3
sucrose catabolism levF med Mannose-specific PTS system component IIC, component of The hexose (glucose and fructose demonstrated) PTS uptake system (characterized) 57% 100% 308.5 Mannose-specific PTS system, IIC component, component of Mannose enzyme II complex, IIAB, IIC, IID. IIC/IID serve allows entry of some bacteriocins including pediocin (class IIa) and lactococcin A (class IIc) (Kjos et al., 2011). Transports and phosphorylates Glucose, Mannose and Glucosamine 73% 386.3
D-glucosamine (chitosamine) catabolism manY med mannose permease IIC component (characterized) 44% 98% 217.6 Mannose-specific PTS system, IIC component, component of Mannose enzyme II complex, IIAB, IIC, IID. IIC/IID serve allows entry of some bacteriocins including pediocin (class IIa) and lactococcin A (class IIc) (Kjos et al., 2011). Transports and phosphorylates Glucose, Mannose and Glucosamine 73% 386.3

Sequence Analysis Tools

View WP_027699770.1 at NCBI

Find papers: PaperBLAST

Find functional residues: SitesBLAST

Search for conserved domains

Find the best match in UniProt

Compare to protein structures

Predict transmenbrane helices: Phobius

Predict protein localization: PSORTb

Find homologs in fast.genomics

Fitness BLAST: loading...

Sequence

MSAISVFFVLIFAFLAGLEGILDQWQLHQPIIASSLIGLATGHLAAGIILGGFLQLIALG
WANVGAAVAPDAALASVASAILMVQSGNFSVENIKGIIIPAAILLATAGLVLTTLVRFFS
VAIVHLADAQAEKGSYRGVAFWHIVALVMQGLRIAIPAGLLLIVPQQVVMHALQSIPPVI
SGGLTIGGGMVVVVGYGMVINLMATRELWPFFFLGFAIAPINQLTLIAIGIIGVVIALVY
LTLQDSNSGSGSNGGGKSGGDPIGDILNEY

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory