GapMind for catabolism of small carbon sources

 

Alignments for a candidate for gltL in Weissella oryzae SG25

Align PEB1C, component of Uptake system for glutamate and aspartate (characterized)
to candidate WP_027698251.1 TT13_RS01035 amino acid ABC transporter ATP-binding protein

Query= TCDB::A3ZI83
         (242 letters)



>NCBI__GCF_000691805.2:WP_027698251.1
          Length = 246

 Score =  207 bits (528), Expect = 1e-58
 Identities = 110/241 (45%), Positives = 159/241 (65%), Gaps = 7/241 (2%)

Query: 1   MIELKNVNKYYGTHHVLKNINLSVKEGEKLVIIGPSGSGKSTTIRCMNGLEEVSSGEVVV 60
           M++L+N+NK Y  H  L+NIN++ +  +  VI+GPSGSGKST +R +N LE+  SG   +
Sbjct: 1   MLKLENINKQYVEHRALENINVTFQSDQTTVIVGPSGSGKSTLLRSLNLLEQPDSGIYTL 60

Query: 61  NNLVLNHKNKIEI-----CRKYCAMVFQHFNLYPHMTVLQNLTLAPMKLQKKSKKEAEET 115
           +N V++    I        R+   MVFQ   ++ H++VL+N+T AP+++ K+ K  A+  
Sbjct: 61  DNQVIDFSQPITTQTLLWTRRQTGMVFQQPRMFDHLSVLKNITEAPIQVLKEPKATAQAE 120

Query: 116 AFKYLKVVGLLDKANVYPATLSGGQQQRVAIARSLCTKKPYILFDEPTSALDPETIQEVL 175
           A K L  VGL D AN YP  LSGGQ QRVAIAR+L  K  Y+L DEPTSALDPE   ++L
Sbjct: 121 ALKLLAAVGLADTANRYPYQLSGGQTQRVAIARALAMKPKYLLLDEPTSALDPELEAQIL 180

Query: 176 DVMKEISHQSNTTMVVVTHEMGFAKEVADRIIFMEDGAIVEENIPSEFFSNPKTERARLF 235
            ++ +I+ +++ TM++VTH M FA+  ADRI+F+EDG I  +  P++FF +P T R   F
Sbjct: 181 HILNQIA-KTDQTMIIVTHNMDFARAAADRILFLEDGQIQFDGTPTDFFDHP-TARIEHF 238

Query: 236 L 236
           L
Sbjct: 239 L 239


Lambda     K      H
   0.317    0.133    0.372 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 171
Number of extensions: 8
Number of successful extensions: 3
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 242
Length of database: 246
Length adjustment: 24
Effective length of query: 218
Effective length of database: 222
Effective search space:    48396
Effective search space used:    48396
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 46 (22.3 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory