Alignments for a candidate for deoD in Lactobacillus oryzae SG293

GapMind for catabolism of small carbon sources

Alignments for a candidate for deoD in Lactobacillus oryzae SG293

Align purine-nucleoside phosphorylase (EC 2.4.2.1) (characterized)
to candidate WP_034529054.1 LOSG293_RS07905 purine-nucleoside phosphorylase

Query= BRENDA::O34925
         (233 letters)



>NCBI__GCF_000740055.1:WP_034529054.1
          Length = 235

 Score =  279 bits (713), Expect = 4e-80
 Identities = 143/231 (61%), Positives = 176/231 (76%), Gaps = 1/231 (0%)

Query: 1   MSVHIGAEKGQIADTVLLPGDPLRAKFIAETYLENVECYNEVRGMYGFTGTYKGKKISVQ 60
           MS H+ A+KG IADTVLLPGDPLRAK+IAET+LEN   YN VR  +G+TGTYKGK+ISVQ
Sbjct: 1   MSEHLEAKKGDIADTVLLPGDPLRAKYIAETFLENPVRYNTVRNAFGYTGTYKGKRISVQ 60

Query: 61  GTGMGVPSISIYVNELIQSYDVQNLIRVGSCGAIRKDVKVRDVILAMTSSTDSQMNRVAF 120
           GTGMG+PSISIY NELI  Y V+ L RVGS G +  DV VRDVILA  ++TDS +    F
Sbjct: 61  GTGMGIPSISIYTNELITEYGVKTLFRVGSSGGMSPDVHVRDVILAQGATTDSSIIHNTF 120

Query: 121 -GSVDFAPCADFELLKNAYDAAKDKGVPVTVGSVFTADQFYNDDSQIEKLAKYGVLGVEM 179
            G + FAP ADF LL  AY AA+D  +PV VG+V + D+FYND+   EKL +YGV   EM
Sbjct: 121 GGGIYFAPIADFGLLDTAYHAAQDLDIPVRVGNVLSEDRFYNDEMDREKLVQYGVAATEM 180

Query: 180 ETTALYTLAAKHGRKALSILTVSDHVLTGEETTAEERQTTFHDMIEVALHS 230
           E  ALY LAAK+  +AL++LT+S+H++TGEETTAEER+ +F DMI+VAL +
Sbjct: 181 EAAALYLLAAKYHVRALAVLTISNHLVTGEETTAEEREKSFDDMIKVALET 231


Lambda     K      H
   0.317    0.133    0.370 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 184
Number of extensions: 6
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 233
Length of database: 235
Length adjustment: 23
Effective length of query: 210
Effective length of database: 212
Effective search space:    44520
Effective search space used:    44520
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.6 bits)
S2: 46 (22.3 bits)

Align candidate WP_034529054.1 LOSG293_RS07905 (purine-nucleoside phosphorylase)
to HMM TIGR00107 (deoD: purine nucleoside phosphorylase (EC 2.4.2.1))

# hmmsearch :: search profile(s) against a sequence database
# HMMER 3.3.1 (Jul 2020); http://hmmer.org/
# Copyright (C) 2020 Howard Hughes Medical Institute.
# Freely distributed under the BSD open source license.
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -
# query HMM file:                  ../tmp/path.carbon/TIGR00107.hmm
# target sequence database:        /tmp/gapView.954335.genome.faa
# - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - - -

Query:       TIGR00107  [M=232]
Accession:   TIGR00107
Description: deoD: purine nucleoside phosphorylase
Scores for complete sequences (score includes all domains):
   --- full sequence ---   --- best 1 domain ---    -#dom-
    E-value  score  bias    E-value  score  bias    exp  N  Sequence                             Description
    ------- ------ -----    ------- ------ -----   ---- --  --------                             -----------
      4e-96  307.0   0.0    4.4e-96  306.9   0.0    1.0  1  NCBI__GCF_000740055.1:WP_034529054.1  


Domain annotation for each sequence (and alignments):
>> NCBI__GCF_000740055.1:WP_034529054.1  
   #    score  bias  c-Evalue  i-Evalue hmmfrom  hmm to    alifrom  ali to    envfrom  env to     acc
 ---   ------ ----- --------- --------- ------- -------    ------- -------    ------- -------    ----
   1 !  306.9   0.0   4.4e-96   4.4e-96       1     229 [.       4     232 ..       4     235 .] 0.96

  Alignments for each domain:
  == domain 1  score: 306.9 bits;  conditional E-value: 4.4e-96
                             TIGR00107   1 hinakkgdiadvvllpGdPlrakyiaekfledakevnevrgmlgftGkykgkkisvmGhGmGipsisiyskel 73 
                                           h++akkgdiad+vllpGdPlrakyiae+fle++  +n vr+ +g+tG+ykgk+isv+G+GmGipsisiy++el
  NCBI__GCF_000740055.1:WP_034529054.1   4 HLEAKKGDIADTVLLPGDPLRAKYIAETFLENPVRYNTVRNAFGYTGTYKGKRISVQGTGMGIPSISIYTNEL 76 
                                           99*********************************************************************** PP

                             TIGR00107  74 ikeyevkkiirvGsCGairkkvklkdviialkastdskvnrvrfv.evdlaaiadfelvklakeaakkkgldv 145
                                           i+ey+vk++ rvGs G ++++v+++dvi+a++a+tds + + +f   + +a+iadf l+ +a++aa++++++v
  NCBI__GCF_000740055.1:WP_034529054.1  77 ITEYGVKTLFRVGSSGGMSPDVHVRDVILAQGATTDSSIIHNTFGgGIYFAPIADFGLLDTAYHAAQDLDIPV 149
                                           ************************************987766665167************************* PP

                             TIGR00107 146 kvGnvfsadlfystdkevldllekygvlavemeaaalyavaaelgkkaltlltvsdhlvthealtaeerqktf 218
                                           +vGnv+s+d fy+++ +  ++l +ygv a emeaaaly +aa+++ +al++lt+s+hlvt e++taeer k+f
  NCBI__GCF_000740055.1:WP_034529054.1 150 RVGNVLSEDRFYNDEMDR-EKLVQYGVAATEMEAAALYLLAAKYHVRALAVLTISNHLVTGEETTAEEREKSF 221
                                           ***************887.56678************************************************* PP

                             TIGR00107 219 kdmielalesv 229
                                           +dmi++ale++
  NCBI__GCF_000740055.1:WP_034529054.1 222 DDMIKVALETA 232
                                           ********986 PP



Internal pipeline statistics summary:
-------------------------------------
Query model(s):                            1  (232 nodes)
Target sequences:                          1  (235 residues searched)
Passed MSV filter:                         1  (1); expected 0.0 (0.02)
Passed bias filter:                        1  (1); expected 0.0 (0.02)
Passed Vit filter:                         1  (1); expected 0.0 (0.001)
Passed Fwd filter:                         1  (1); expected 0.0 (1e-05)
Initial search space (Z):                  1  [actual number of targets]
Domain search space  (domZ):               1  [number of targets reported over threshold]
# CPU time: 0.00u 0.01s 00:00:00.01 Elapsed: 00:00:00.00
# Mc/sec: 13.39
//
[ok]

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources