Alignments for a candidate for serP in Lactobacillus oryzae SG293

GapMind for catabolism of small carbon sources

Alignments for a candidate for serP in Lactobacillus oryzae SG293

Align Serine transporter, SerP2 or YdgB, of 459 aas and 12 TMSs (Trip et al. 2013). Transports L-alanine (Km = 20 μM), D-alanine (Km = 38 μM), L-serine, D-serine (Km = 356 μM) and glycine (Noens and Lolkema 2015). The encoding gene is adjacent to the one encoding SerP1 (TC# 2.A.3.1.21) (characterized)
to candidate WP_034528522.1 LOSG293_RS06885 amino acid permease

Query= TCDB::F2HQ24
         (457 letters)



>NCBI__GCF_000740055.1:WP_034528522.1
          Length = 448

 Score =  359 bits (922), Expect = e-104
 Identities = 190/440 (43%), Positives = 267/440 (60%), Gaps = 3/440 (0%)

Query: 7   EENKPSQRGLKNRHIQLIAIAGTIGTGLFLGAGKSIHLTGPSIIFVYLIIGALMYILLRA 66
           E+     R LK+RHIQ+IAI G IGTGLFLG+G +I   GPSII  YLI+G   + L+RA
Sbjct: 2   EDKLKLSRSLKSRHIQMIAIGGAIGTGLFLGSGSAIRAAGPSIILSYLIVGIFCFFLMRA 61

Query: 67  IGEMLYQDPNQHSFLNFVSRYLGEKPGYFIQWSYLLVVVFVAMAELIAIGTYINFWLPDL 126
           IGE+L  D ++ SF++F+  YLG++  +   W+Y    + ++MA+L A G Y+ +W P+ 
Sbjct: 62  IGELLLSDTSKASFIDFIKEYLGDRAEFITGWTYWSCWISLSMADLTATGIYVKYWFPNF 121

Query: 127 PIWMTEVFVLVLLTLLNTLNPKFFGETEFWFGMIKIVAIIGLILTAIILIFSHYHTGTDT 186
           P W+T + +++LL+L+N  N   FGE E WF +IK+ AII LI   + L F H+     T
Sbjct: 122 PQWLTPLIIILLLSLINLANVGLFGEFESWFSLIKVAAIIALIAIGLFLAFGHFKIAGST 181

Query: 187 VSVTNITKGFEFFPNGLSNFFESFQMVMFAFVSMEFIGMTAAETDNPRPTLKKAINQIPI 246
               N+     FFP G+S F  SFQMV+FAFV +E +G+T  ET NP   L KAIN +PI
Sbjct: 182 TGFANLVNHGGFFPTGISGFLLSFQMVVFAFVGIEMVGLTVGETSNPEENLPKAINSLPI 241

Query: 247 RIVLFYVGALLAIMSIYQWRDIPADKSPFVTIFQLIGIKWAAALVNFVVLTSAASALNSA 306
           RI LFYVG+++AIMS+Y W  I  + SPFV +F  IGI  AA ++NFVVLT+A SA NSA
Sbjct: 242 RIGLFYVGSMIAIMSVYPWDKITTNVSPFVQVFSGIGIAGAAGILNFVVLTAAMSATNSA 301

Query: 307 LFSITRNLYSLSKLNNDKILKPFTKFSKAGVPVNALLFTSLLILFTPFISMIPAISNSFV 366
           +FS +R L++L+   N    K F +  K  VP  AL  +S LILF   +      +  F 
Sbjct: 302 IFSTSRTLHALASGGNAP--KRFRELDKRAVPSKALNVSS-LILFVIVVLNFVMPAQIFT 358

Query: 367 FITSVATNLFLVVYLMTLITYLKYRKSSDFDPKGFVLPAAHIFIPLAIAGFVLIFISLFC 426
            I+ V+T  F+ V+++ L  +LKYR+    D   F +P   +   L +  F+ I I LF 
Sbjct: 359 LISGVSTINFVFVWVVLLWCHLKYRQQHRDDHPSFTMPGYPLTDYLTLIFFIGILIYLFF 418

Query: 427 FKDTIVPAIGSVIWVLIFGL 446
             +T V  I S+IW +   +
Sbjct: 419 VPETRVSLIISIIWFIALAI 438


Lambda     K      H
   0.330    0.144    0.431 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 558
Number of extensions: 26
Number of successful extensions: 2
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 457
Length of database: 448
Length adjustment: 33
Effective length of query: 424
Effective length of database: 415
Effective search space:   175960
Effective search space used:   175960
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 15 ( 7.1 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 40 (21.8 bits)
S2: 51 (24.3 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources