Alignments for a candidate for edd in Hippea jasoniae Mar08-272r

GapMind for catabolism of small carbon sources

Alignments for a candidate for edd in Hippea jasoniae Mar08-272r

Align Phosphogluconate dehydratase; 6-phosphogluconate dehydratase; EC 4.2.1.12 (characterized)
to candidate WP_035586458.1 EK17_RS00375 dihydroxy-acid dehydratase

Query= SwissProt::P21909
         (607 letters)



>NCBI__GCF_000744435.1:WP_035586458.1
          Length = 555

 Score =  234 bits (598), Expect = 6e-66
 Identities = 171/521 (32%), Positives = 260/521 (49%), Gaps = 34/521 (6%)

Query: 64  DFNRMNIGVVTSYNDMLSAHEPYYRYPEQMKVFAREVGATVQVAGGVPAMCDGVTQGQPG 123
           + NR  I +  SYN ++  H    +  E +K      G T  +   V  + DG+  G  G
Sbjct: 30  ELNRPIIAIANSYNTIVPGHVHMQQLVEAVKYGIYMAGGT-PIEFNVIGVDDGIAMGHLG 88

Query: 124 MEESLFSRDVIALATSVSLSHGMFEGAALLGICDKIVPGLLMGALRFGHLPTILVPSGPM 183
           M  SL SR++IA +    ++    +G  +L  CDKIVPG++M A R  ++P I++  GPM
Sbjct: 89  MHYSLPSRELIADSVETMVNAHPVDGLVILPACDKIVPGMMMAAARV-NIPAIMISGGPM 147

Query: 184 TTGIPNKEKIRIRQL------YAQGKIGQKELLDMEAACYHAEGTCTFYGTANTNQMVME 237
             G  +   I + Q+      Y  G+I ++EL  +E A   + G+C+   +AN+   + E
Sbjct: 148 LAGRFSGTDIDLAQVFEAVGKYVNGEITEEELRAVEEAGCPSCGSCSGMYSANSINCISE 207

Query: 238 VLGLHMPGSAFVTPGTPLRQALTRAAVHRVAELGWKGDDYRPLGKIIDEKSIVNAIVGLL 297
           VLGL +PG+  +      R  L + A  ++ EL  KG   R    I+ +++ +NAIV  +
Sbjct: 208 VLGLSLPGNGTIPAPMAARVRLAKLAGMKIVELVEKGIKPRD---IMTKEAFLNAIVADM 264

Query: 298 ATGGSTNHTMHIPAIARAAGVIVNWNDFHDLSEVVPLIARIYPNGPRDINEFQNAGGMAY 357
           A G STN  +H+ AIA  A V +  +DF  L++ VP +    P GP  + +   AGGM  
Sbjct: 265 AMGCSTNTVLHLLAIANEAEVDLTLDDFDKLTDKVPDLCAFSPVGPYHLQDLDEAGGMMA 324

Query: 358 VIKELLSANLLNRDVTTIA-KGGIEEYAKAPALNDAGELVWKPAGEPGDDTILRPVSNPF 416
           ++  L    LLN D  T++ K   E Y  A                   D ++RP+  P+
Sbjct: 325 LLNRLEPLGLLNHDCITVSGKTIYENYKNARVYR---------------DEVIRPLDKPY 369

Query: 417 AKDGGLRLLEGNLG--RAMYKASAVDPKFWTIEAPVRVFSDQDDVQKAFKAGELNKDVIV 474
              GGL +L+GNL    A+ K S VD          RVF  +++  KA  A +++K  +V
Sbjct: 370 FPRGGLTILKGNLAPDGAVLKQSGVDVSMNKFVGRARVFDSEEEALKAIFAKDIHKGDVV 429

Query: 475 VVRFQGPRAN-GMPELHKLTPAL-GVLQDNGYKVALVTDGRMSGATGKVPVALHVSPEAL 532
           V+R++GP    GM E+ + T A+ G+  D    VAL+TDGR SGAT  + V  H++PEA 
Sbjct: 430 VIRYEGPAGGPGMKEMLQPTSAIAGMGLDK--DVALITDGRFSGATRGLSVG-HIAPEAA 486

Query: 533 GGGAIGKLRDGDIVRISVEEGKLEALVPADEWNARPHAEKP 573
            GG IG + +GD +   VE   +  LV       R    KP
Sbjct: 487 RGGLIGLIEEGDKIEFDVENKTMNLLVDEKTIEQRRKNFKP 527


Lambda     K      H
   0.318    0.136    0.403 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 868
Number of extensions: 49
Number of successful extensions: 8
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 607
Length of database: 555
Length adjustment: 36
Effective length of query: 571
Effective length of database: 519
Effective search space:   296349
Effective search space used:   296349
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 53 (25.0 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

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About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources