Alignments for a candidate for mglA in Hippea jasoniae Mar08-272r

GapMind for catabolism of small carbon sources

Alignments for a candidate for mglA in Hippea jasoniae Mar08-272r

Align Monosaccharide-transporting ATPase, component of Glucose porter. Also bind xylose (Boucher and Noll 2011). Induced by glucose (Frock et al. 2012). Directly regulated by glucose-responsive regulator GluR (characterized)
to candidate WP_035588821.1 EK17_RS06470 ABC transporter ATP-binding protein

Query= TCDB::G4FGN3
         (494 letters)



>NCBI__GCF_000744435.1:WP_035588821.1
          Length = 502

 Score =  311 bits (796), Expect = 4e-89
 Identities = 178/499 (35%), Positives = 297/499 (59%), Gaps = 12/499 (2%)

Query: 4   ILEVKSIHKRFPGVHALKGVSMEFYPGEVHAIVGENGAGKSTLMKIIAGVYQPDEGEIIY 63
           IL+  +I KRF  V A   V+ E   GE+H ++GENGAGK+TLM I+ G+Y P EG+I  
Sbjct: 5   ILKAVNITKRFGDVIANDSVNFELNRGEIHTLLGENGAGKTTLMNILYGIYLPSEGQIYL 64

Query: 64  EGRGVRWNHPSEAINAGIVTVFQELSVMDNLSVAENIFMGDEEKRGIFIDYKKMYREAEK 123
           E + V  N P +AI  GI  + Q   ++D L+V ENI +G +E  GI I  K+   +  K
Sbjct: 65  EEKPVVINSPLDAIRLGIGMIHQHFMLVDTLTVYENIILGLKEY-GIIIK-KRAILQKLK 122

Query: 124 FMKEEFGIEIDPEEKLGKYSIAIQQMVEIARAVYKKAKVLILDEPTSSLTQKETEKLFEV 183
            +++ + ++++P EK+ + ++  QQ VEI + +++ A +LILDEPT+ LT +ET+ LF +
Sbjct: 123 EIEDRYNLKVNPFEKIWQLAVGEQQKVEIIKVLFRGANILILDEPTAVLTPQETKSLFNI 182

Query: 184 VKSLKEKGVAIIFISHRLEEIFEICDKVSVLRDGEYIGTDSIENLTKEKIVEMMVGRKLE 243
           +K++K    +IIFISH+LEE+ +I D+++V+R G  I T + +N +KE++ ++M+   ++
Sbjct: 183 LKNMKNNSKSIIFISHKLEEVLQISDRITVMRAGRIIKTLNKQNTSKEQLADLMMA-DIK 241

Query: 244 KFYIKEAHEPG-EVVLEVKNL------SGERFENVSFSLRRGEILGFAGLVGAGRTELME 296
              I + ++P  E +LEV NL        +  + +S  ++RGEILG AG+ G G+ EL++
Sbjct: 242 LLNIPKENKPSDETILEVNNLQVISDKGVKSVDGLSLKVKRGEILGIAGVSGNGQKELVQ 301

Query: 297 TIFGFRPKRGGEIYIEGKRVEINHPLDAIEQGIGLVPEDRKKLGLILIMSIMHNVSLPSL 356
           TI G +   GG I   G  +    P    + GI  +P DR  +G+   ++ + N  L   
Sbjct: 302 TIAGVKKAVGGRIIFNGIDITNKPPRFIAKSGINYIPADRLAMGVAPNLNCVDNAILRCY 361

Query: 357 DR--IKKGPFISFKREKELADWAIKTFDIRPAYPDRKVLYLSGGNQQKVVLAKWLALKPK 414
            R    +   + +K+  +     +K ++++       +  LSGGN QK++LA+ L  KPK
Sbjct: 362 YRKPFLQNGIMKYKKAIDYTKQIVKDYNVKLKSIFSPIKLLSGGNMQKLILARELLEKPK 421

Query: 415 ILILDEPTRGIDVGAKAEIYRIMSQLAKEGVGVIMISSELPEVLQMSDRIAVMSFGKLAG 474
           +LI   PTRG+D+ +     + + ++AKEG  VI++S +L E+L +SDR+ VM  GK+ G
Sbjct: 422 LLIASYPTRGLDLASTIFFRKKLVEIAKEGTTVILVSEDLDEILSLSDRVVVMYEGKIVG 481

Query: 475 IIDAKEASQEKVMKLAAGL 493
           I++ K   +  +  + AG+
Sbjct: 482 IVNPKTTDKNTLGLMMAGI 500


Lambda     K      H
   0.318    0.138    0.385 

Gapped
Lambda     K      H
   0.267   0.0410    0.140 


Matrix: BLOSUM62
Gap Penalties: Existence: 11, Extension: 1
Number of Sequences: 1
Number of Hits to DB: 634
Number of extensions: 31
Number of successful extensions: 9
Number of sequences better than 1.0e-02: 1
Number of HSP's gapped: 1
Number of HSP's successfully gapped: 1
Length of query: 494
Length of database: 502
Length adjustment: 34
Effective length of query: 460
Effective length of database: 468
Effective search space:   215280
Effective search space used:   215280
Neighboring words threshold: 11
Window for multiple hits: 40
X1: 16 ( 7.3 bits)
X2: 38 (14.6 bits)
X3: 64 (24.7 bits)
S1: 41 (21.7 bits)
S2: 52 (24.6 bits)

This GapMind analysis is from Sep 24 2021. The underlying query database was built on Sep 17 2021.

Downloads

Candidates (tab-delimited)
Steps (tab-delimited)
Rules (tab-delimited)
Protein sequences (fasta format)
Organisms (tab-delimited)
SQLite3 databases

Related tools

About GapMind

Each pathway is defined by a set of rules based on individual steps or genes. Candidates for each step are identified by using ublast (a fast alternative to protein BLAST) against a database of manually-curated proteins (most of which are experimentally characterized) or by using HMMer with enzyme models (usually from TIGRFam). Ublast hits may be split across two different proteins.

A candidate for a step is "high confidence" if either:

ublast finds a hit to a characterized protein at above 40% identity and 80% coverage, and bits >= other bits+10.
- (Hits to curated proteins without experimental data as to their function are never considered high confidence.)
HMMer finds a hit with 80% coverage of the model, and either other identity < 40 or other coverage < 0.75.

where "other" refers to the best ublast hit to a sequence that is not annotated as performing this step (and is not "ignored").

Otherwise, a candidate is "medium confidence" if either:

ublast finds a hit at above 40% identity and 70% coverage (ignoring otherBits).
ublast finds a hit at above 30% identity and 80% coverage, and bits >= other bits.
HMMer finds a hit (regardless of coverage or other bits).

Other blast hits with at least 50% coverage are "low confidence."

Steps with no high- or medium-confidence candidates may be considered "gaps." For the typical bacterium that can make all 20 amino acids, there are 1-2 gaps in amino acid biosynthesis pathways. For diverse bacteria and archaea that can utilize a carbon source, there is a complete high-confidence catabolic pathway (including a transporter) just 38% of the time, and there is a complete medium-confidence pathway 63% of the time. Gaps may be due to:

our ignorance of proteins' functions,
omissions in the gene models,
frame-shift errors in the genome sequence, or
the organism lacks the pathway.

GapMind relies on the predicted proteins in the genome and does not search the six-frame translation. In most cases, you can search the six-frame translation by clicking on links to Curated BLAST for each step definition (in the per-step page).

For more information, see:

the paper from 2019 on GapMind for amino acid biosynthesis
the 2024 update on GapMind for amino acid biosynthesis
the paper from 2022 on GapMind for carbon sources
the source code
instructions for running GapMind on your computer

If you notice any errors or omissions in the step descriptions, or any questionable results, please let us know

by Morgan Price, Arkin group, Lawrence Berkeley National Laboratory

GapMind for catabolism of small carbon sources